Routine sample preparation and HPLC analysis for ascorbic acid (vitamin C) determination in wheat plants andArabidopsisleaf tissues

Abstract

Running titleRoutine HPLC analysis for ascorbic acid SummaryPlants have developed various mechanisms to protect themselves against oxidative stress. One of the most important non-enzymatic antioxidants is ascorbic acid. There is thus a need for a rapid, sensitive method for the analysis of the reduced and oxidised forms of ascorbic acid in crop plants. In this paper a simple, economic, selective, precise and stable HPLC method is presented for the detection of ascorbate in plant tissue. The sensitivity, the short retention time and the simple isocratic elution mean that the method is suitable for the routine quantification of ascorbate in a high daily sample number. The method has been found to be better than previously reported methods, because of the use of an economical, readily available mobile phase, UV detection and the lack of complicated extraction procedures. The method has been tested on Arabidopsis plants with different ascorbate levels and on wheat plants during Cd stress.

Salicylic Acid and Sodium Salicylate Alleviate Cadmium Toxicity to Different Extents in Maize (Zea mays L.)

“…The plant samples were ground to a fine powder using liquid nitrogen and the AsA was extracted from 200 mg samples with 1.5 ml 1.5% metaphosphoric acid. The AsA content was measured as described earlier by Szalai et al [ 24 ].…”

Identification of a redox-dependent regulatory network of miRNAs and their targets in wheat

“…The concentration of dehydroascorbate (DHA), a two-electron oxidized form of AsA was estimated by subtracting the reduced portion from the total AsA pool (Szalai et al, 2014).…”

Phytoremediation capacity of aquatic plants is associated with the degree of phytochelatin polymerization

“…For the measurement of reduced ascorbic acid, 20 l water was added instead of dithiotreitol. The samples were analysed by HPLC (Waters, Milford, MA, USA) using a diode array (W996) detector as described previously [27].…”