Rp-HPLC Method for the Simultaneous Estimation of Lisinopril and Diuretics in Bulk, Dosage Forms and Human Serum

Abstract

A new, simple, specific and precise assay method has been developed and validated on an isocratic RP-HPLC for the simultaneous determination of lisinopril (LIS) and diuretics (hydrochlorothiazide (HCT) and furosemide (FUR)) in bulk, dosage formulations and human serum at 225nm. Chromatographic separation was achieved by using Purospher Star C 18 (250 mm x 4.6 mm, 5 µm) column, mobile phase consisting of methanol: water: acetonitrile (80:15:5 v/v/v) adjusted to pH 3.0 via phosphoric acid (85%) having a flow rate of 1.0 mL min -1 at room temperature. The retention times for lisinopril, hydrochlorothiazide and furosemide were 2.6, 3.2 and 4.1 min, respectively. Calibration curves were linear over range of 0.25-10 µgmL -1 with a correlation coefficient ±0.999.LOD and LOQ were in the ranges of 0.68-2.07 µgmL -1 . Intra and inter-run precision and accuracy results were 98.32 to 100.7 %. Therefore, the developed method could be used for routine estimation of lisinopril and diuretics (HCT and FUR) alone or in combination in bulk materials, pharmaceutical dosage formulations, human serum and also for the clinical investigations.