RP‐HPTLC densitometric determination and validation of vanillin and related phenolic compounds in accelerated solvent extract of Vanilla planifolia*

Abstract

A simple, fast and sensitive RP-HPTLC method is developed for simultaneous quantitative determination of vanillin and related phenolic compounds in ethanolic extracts of Vanilla planifolia pods. In addition to this, the applicability of accelerated solvent extraction (ASE) as an alternative to microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE) and Soxhlet extraction was also explored for the rapid extraction of phenolic compounds in vanilla pods. Good separation was achieved on aluminium plates precoated with silica gel RP-18 F(254S) in the mobile phase of methanol/water/isopropanol/acetic acid (30:65:2:3, by volume). The method showed good linearity, high precision and good recovery of compounds of interest. ASE showed good extraction efficiency in less time as compared to other techniques for all the phenolic compounds. The present method would be useful for analytical research and for routine analysis of vanilla extracts for their quality control.

Comparison of extraction techniques and modeling of accelerated solvent extraction for the authentication of natural vanilla flavors

“…The blend was introduced into 33 or 100 mL cells and extracted with the ASE-100 system (Dionex, Olten, Switzerland). The best ASE condition found by Sharma et al [38], i. e., ethanol dehydrated, 608C, two cycles of 5-min extraction time, was tested and the results compared with ours. The ASE extraction from yogurts and ice cream was made with ethyl acetate under 1008C using four cycles of 3 min.…”

“…Accelerated solvent extraction (ASE) has recently been applied to extract the phenolic compounds from vanilla pods [38]. The method was compared with MAE, UAE, and Soxhlet with ethanol.…”

Ferulic acid in cereals - a review

“…To determine the amount of ferulic acid it is possible to use high-performance liquid chromatography (HPLC) (Kováčová & Malinová 2007;Laokuldilok et al 2011), thin-layer chromatography (TLC) (Sharma et al 1998), high-performance thin layer chromatography (HPTLC) (Sharma et al 2007), capillary tube electrophoresis (Sharma et al 2007;Aturki et al 2008), and colorimetry (Garcia et al 2002;Tee-ngam et al 2013). HPLC currently represents the most popular technique for analysis of phenolic compounds.…”

Method development for simultaneous detection of ferulic acid and vanillin using high-performance thin layer chromatography

“…Different approaches such as UV spectrophotometry (Mabry et al 1970;Macheix et al 1990) gas chromatography (GC), capillary electrophoresis (CE), high-pressure liquid chromatography (HPLC), thin layer chromatography (TLC), and high-performance thin layer chromatography (HPTLC) are some of the frequently used methods for the detection, qualitative analysis, and quantification (Sharma et al 2007).…”