RP‐thin layer chromatographic method for the quantification of three gingerol homologs of ultrasonic‐assisted fresh rhizome extracts of Zingiber officinale collected from North Western Himalayas

Abstract

A rapid and sensitive RP high-performance thin-layer chromatographic (RP-HPTLC) methodology was developed and validated for the quantitative estimation of gingerols in methanolic extract of fresh ginger rhizome. The samples were chromatographed on RP-TLC glass plates pre-coated with RP-18 60F(254) as the stationary phase. Linear ascending development was carried out in twin trough glass chamber saturated with ternary-solvent system consisting of acetonitrile-water-formic acid (7:2:1 v/v/v) at room temperature (25+/-2 degrees C) and plates were scanned at 500 nm. The system was found to give compact spots for gingerols (R(f) values of 6-gingerol 0.73+/-0.04, 8-gingerol 0.59+/-0.08 and 10-gingerol 0.36+/-0.05). Linearity was found to be in the range of 140-840 ng/spot for 6-gingerol, 168-1008 ng/spot for 8-gingerol and 136-816 ng/spot for 10-gingerol with significantly high value of correlation coefficient. The linear regression analysis data for the calibration plots showed linear relationship (r(2)) and ranged from 0.9992 to 0.9937 for 6-, 8- and 10-gingerol. The method was used for routine analyses and to obtain relative amounts of the gingerols in the fresh rhizomes of the ginger cultivated in different locations of Uttarakhand and Himachal Pradesh of North Western Himalayas (India).

Ultrasound-assisted extraction and fast chromolithic method development, validation and system suitability analysis for 6, 8, 10-gingerols and shogaol in rhizome of Zingiber officinale by liquid chromatography-diode array detection

“…[10] A large number of HPLC and HPTLC methods for the quantification of 6-gingerol in [11,12] ginger oil, [13] crude drugs drink, [14] commercial Ayurvedic formulation [15] and rhizome [16] have been reported in the literature. The literature survey also revealed that quantification of other ginger homologues (8,10 gingerols) with 6-gingerol, [17] other diterpenes in Japanese gingers, [18] green and dried ginger, [19] freeze dried CO 2 extract, [20] three gingerols [21] with 6-shogaol in Jamaican ginger, [22] commercial ginger, fresh and dried rhizome and dietary supplements, [23][24][25] with have also been reported. Until date, no report on chromolithic separation of 6, 8, 10-gingerols and shogaol for the quantification, validation and system suitability in the ginger rhizome has been reported.…”

Simultaneous quantification of five marker compounds of Betula utilis stem bark using a validated high‐performance thin‐layer chromatography method

“…HPTLC is a globally accepted rational and practical solution to characterize the crude plant drug, pharmacologically active constituents, enriched standardized extracts and their formulations. In continuation of our study 19, 20, on application of planar chromatography for fingerprinting and chromatographic method development to assure quality of medicinal plants, we report herein a rapid, precise, sensitive and validated high‐performance thin‐layer chromatographic method for triterpenoid's quantitative determination in B. utilis stem bark extracts.…”

Simultaneous Determination of 6-Shogaol and 6-Gingerol in Various Ginger (Zingiber officinale Roscoe) Extracts and Commercial Formulations Using a Green RP-HPTLC-Densitometry Method

“…Some high-performance thin layer chromatography (HPTLC) methods have also been reported for the determination of 6-GIN in ginger extract, commercial foods, and commercial formulations [ 20 , 21 , 22 ]. The HPTLC method had also been utilized for the simultaneous determination of 6-GIN, 8-GIN, and 10-GIN in ultrasonication-assisted extract of ginger [ 23 ]. The HPTLC technique was also applied for the determination of a similar compound 8-GIN in plant extracts, commercial foods, and commercial formulations [ 24 ].…”