Rpr- and hid-driven cell death in Drosophila photoreceptors

Hsu, Cheng Da; Adams, Sheila M.; O'Tousa, Joseph E.

doi:10.1016/s0042-6989(01)00231-0

Cited by 9 publications

(11 citation statements)

References 42 publications

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“…We verified that the diap1 and DroncDN transgenes were active by showing they partially protect from GMRhid-driven cell death using the rescue of the small eye phenotype as described previously (Hay et al, 1995;Hawkins et al, 2000). However, unlike P35 (Hsu et al, 2002), they were not effective in the rescue of ninaEhid (data not shown).…”

Section: Methodssupporting

confidence: 75%

“…Figure 3A shows data collected from observation of the DPP structure in this experiment. We confirmed that GMRp35 was capable of suppressing photoreceptor degeneration triggered by hid expression under control of the ninaE rhodopsin promoter (Hsu et al, 2002). In contrast, the DPP was retained for only ϳ1 d longer in norpA with P35 than seen in norpA flies lacking P35.…”

Section: Limited P35 Suppression Of Norpa Degenerationsupporting

confidence: 72%

“…1C,D), rhabdomeres are further deteriorated, and cell bodies are shrunken with many aberrant vesicles and electron-dense material. Similar morphological changes are seen in adult photoreceptors destined to die because of either the Hid or Rpr proteins (Hsu et al, 2002). Figure 1, C and D, documents the engulfment of the dying norpA photoreceptors.…”

Section: Norpa Photoreceptors Show Morphological Features Of Programmsupporting

confidence: 58%

“…In addition, P35 expressed from the GMR and the major rhodopsin promoter, which is active throughout adult life, show similar suppression of Rpr-and Hid-triggered photoreceptor degeneration (Hsu et al, 2002). In these experiments, the rhodopsin promoter drove Rpr or Hid expression exclusively in photoreceptors after retinal cell type specification and continued to be expressed throughout the adult life.…”

Section: Discussionmentioning

confidence: 65%

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Limited Role of Developmental Programmed Cell Death Pathways inDrosophila norpARetinal Degeneration

Hsu¹,

Whaley²,

Frazer³

et al. 2004

J. Neurosci.

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We examined the role of programmed cell death (PCD) pathways in retinal degeneration caused by a mutation in the norpA gene. norpA degeneration shows morphological hallmarks of programmed cell death, specifically cytoplasmic condensation and engulfment of the dying photoreceptor cells by neighboring retinal pigment cells. However, genetic mosaic analysis of adult photoreceptors lacking rpr, hid, and grim show that these PCD inducers are not required for norpA degeneration. We showed previously that ectopic expression of either rpr or hid triggers rapid PCD in adult photoreceptors, and this is completely suppressed by the coexpression of the baculoviral P35 caspase inhibitor. In contrast, expression of P35 does not suppress norpA retinal degeneration, although a small delay in the rate of degeneration is observed in low light-low temperature conditions. P35 does not alter the morphological characteristics of norpA cell death. Overexpression of the Drosophila inhibitor of apoptosis Diap1 or a dominant-negative form of the Dronc caspase, even when coexpressed with P35, does not dramatically alter the time course of norpA degeneration. These results establish that the pathways responsible for PCD in development do not play a major role in adult retinal degeneration caused by norpA.

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Section: Methodssupporting

confidence: 75%

Section: Limited P35 Suppression Of Norpa Degenerationsupporting

confidence: 72%

Section: Norpa Photoreceptors Show Morphological Features Of Programmsupporting

confidence: 58%

Section: Discussionmentioning

confidence: 65%

See 2 more Smart Citations

Limited Role of Developmental Programmed Cell Death Pathways inDrosophila norpARetinal Degeneration

Hsu¹,

Whaley²,

Frazer³

et al. 2004

J. Neurosci.

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show abstract

“…No retinular or ocellar fibers were observed in adult GMR-hid homozygotes with an anti-chaoptin antibody or in adult GMR-hid heterozygotes with a Rh6-gfp reporter gene (data not shown). The complete disappearance of all photoreceptor cell bodies expressing hid from Rh1 promoter sequences has been similarly reported recently (Hsu et al, 2002). w norpA p24 (Pearn et al, 1996) (Renn et al, 1999), yw; tim-gal4 (Kaneko, 1998), and yw; UAS-cry (Emery et al, 2000b) flies have been described previously.…”

Section: Methodssupporting

confidence: 72%

Novel Features of Cryptochrome-Mediated Photoreception in the Brain Circadian Clock ofDrosophila

Malpel²,

et al. 2004

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In Drosophila, light affects circadian behavioral rhythms via at least two distinct mechanisms. One of them relies on the visual phototransduction cascade. The other involves a presumptive photopigment, cryptochrome (cry), expressed in lateral brain neurons that control behavioral rhythms. We show here that cry is expressed in most, if not all, larval and adult neuronal groups expressing the PERIOD (PER) protein, with the notable exception of larval dorsal neurons (DN2s) in which PER cycles in antiphase to all other known cells. Forcing cry expression in the larval DN2s gave them a normal phase of PER cycling, indicating that their unique antiphase rhythm is related to their lack of cry expression. We were able to directly monitor CRY protein in Drosophila brains in situ. It appeared highly unstable in the light, whereas in the dark, it accumulated in both the nucleus and the cytoplasm, including some neuritic projections. We also show that dorsal PER-expressing brain neurons, the adult DN1s, are the only brain neurons to coexpress the CRY protein and the photoreceptor differentiation factor GLASS. Studies of various visual system mutants and their combination with the cry b mutation indicated that the adult DN1s contribute significantly to the light sensitivity of the clock controlling activity rhythms, and that this contribution depends on CRY. Moreover, all CRY-independent light inputs into this central behavioral clock were found to require the visual system. Finally, we show that the photoreceptive DN1 neurons do not behave as autonomous oscillators, because their PER oscillations in constant darkness rapidly damp out in the absence of pigment-dispersing-factor signaling from the ventral lateral neurons.

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Drosophila mosaic screen identifies diehard mutants as norpA P24 suppressors

Lee

2012

Genes Genom

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Rpr- and hid-driven cell death in Drosophila photoreceptors

Cited by 9 publications

References 42 publications

Limited Role of Developmental Programmed Cell Death Pathways inDrosophila norpARetinal Degeneration

Limited Role of Developmental Programmed Cell Death Pathways inDrosophila norpARetinal Degeneration

Novel Features of Cryptochrome-Mediated Photoreception in the Brain Circadian Clock ofDrosophila

Drosophila mosaic screen identifies diehard mutants as norpA P24 suppressors

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