Rs‐198 Liquid Biofertilizers Affect Microbial Community Diversity and Enzyme Activities and Promote Vitis vinifera L. Growth

Abstract

Chemical fertilizers were applied on perennial tree vines to obtain high yields, which have resulted in considerable deterioration of soil quality, and it is likely to have negative impacts on the development of the grape industry. In this study, P. putida Rs-198 liquid biofertilizer (Rs198LBF) was inoculated into grape rhizosphere soils to assess its influence on grape growth and microbial community. Field experiment results showed that grape growth and quality increased depending on the concentrations of Rs198LBF applications. The berry weight, length, and width in addition with 60 ml Rs198LBF (1.44×1013 cfu ml−1 Rs-198) per grapevine treatment (BFP3) were 17.2%, 6.2%, and 4.4% higher than those of CK (control, non-inoculation) treatment, respectively. The available phosphorus contents in addition with 40 ml Rs198LBF per grapevine (BFP2) and BFP3 treatments were 12.6% and 55.3% higher than those of CK treatment (P<0.05). The activities of invertase and alkaline phosphatase were improved in BFP2 and BFP3 treatment compared with those in CK. The relative abundance of potentially beneficial bacteria significantly increased compared with that in CK treatment (P<0.05). The clusters of orthologous groups (COG) annotation illustrated that the application of 60 ml Rs198LBF increased the relative abundance of metabolic genes in rhizosphere soil. The results of this study show that biofertilizer is very effective in enhancing plant growth and affects soil community diversity.

Application of biostimulant products and biological control agents in sustainable viticulture: A review

“…Erdogan et al (2018) achieved similar improvements with combinations of a wide range of PGPR strains in grafted rootstocks of V. berlandieri and V. riparia in Turkey. In China, Lu et al (2020) observed enhanced growth and final grape quality, following the application of P. putida Rs-198 to a perennial variety.…”

Liquid biofertilizers as a sustainable solution for agriculture

“…In organic fertilizers, some organisms like earthworms need to convert the fertilizer into useful material which plants can absorb easily. Plant-growth promoting rhizobacteria is the most used bacteria in producing biofertilizers since it enhances plant growth by releasing potassium (K), fixing nitrogen (N), solving phosphorus (P), and producing hormones ( Lu et al., 2020 ). Biofertilizers come in solid, liquid, polymer entrapped formulations, and fluidized bed dry formulations ( Mącik et al., 2020 ).…”

“… Lu et al. (2020) did an experiment on Vitis vinifera L. (grapes) by preparing four different concentrations of P. putida Rs-198 in a nutrient agar liquid medium.…”

A Comparative Analysis of Microbe-Based Technologies Developed at ICAR-NBAIM Against Erysiphe necator Causing Powdery Mildew Disease in Grapes (Vitis vinifera L.)

“…Resistance development in the pathogens and residual toxicity of chemical fungicides on the environment and human health have compelled researchers and commercial grape growers to look for alternative strategies (Yildirim and Dardeniz, 2010 ; Miles et al, 2012 ; Fernández-González et al, 2013 ; Çetinkaya and Fadime, 2016 ). With the possible withdrawal of chemical fungicides, including sulfur powder, from the schedule of the acceptable input chart and the demand for residue-free grapes, there is an urgent need to find suitable alternatives for disease management in the organic systems of viticulture (Carisse et al, 2009 ; Yildirim and Dardeniz, 2010 ; Lu H. et al, 2020 ). Among them, the development of resistant cultivars with a high degree of resistance/tolerance to respective pathogens to produce high-quality grapes and wines commensurate with the parameters for higher standards of food safety is of great importance (Pap et al, 2016 ; Riaz et al, 2020 ).…”