RT-PCR detection and molecular characterization ofBanana bract mosaic virusfrom the pseudostem and bract of banana

Significant plant virus diseases in India and a glimpse of modern disease management technology

“…The PCR yielded a fragment of 900 nt for the CP-coding region and 162 nt for the 3 0 UTR. The isolate had 98.1% and 99% homology with other Indian isolates, indicating a common origin (Sankaralingam et al 2006). The virus titer is higher in the bracts and mid rib than in the leaf sheath.…”

“…This virus is transmitted by Rhopalosiphum maidi, Aphis gossypii and Pentalonia nigronervosa in a nonpersistent manner. Presence of BBrMV can be detected by serology using ELISA Singh 2007) and has been detected from pseudostems and banana bracts using RT-PCR (Sankaralingam et al 2006;.…”

Recombinant Coat Protein of Banana Bract Mosaic Virus as a Potential Antigen for Serological Detection of the Virus

“…The cDNA was used as template for Polymerase Chain Reaction (PCR). The coat protein gene of 12 isolates were amplified using forward primer and reverse primer (Table 1) [26] under the following conditions: 94°C for 2 min, followed by 35 cycles of 94°C for 1 min, 42°C for 1 min, 72°C for 2 min with a final extension for 10 min at 72°C. The Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) products were separated by 1.2% Agarose gel and purified PCR products (PCR purification kit, ThermoFisher Scientific) were sequenced by Sanger dideoxy sequencing method.…”