Ru(bpy)32+/β-cyclodextrin-Au nanoparticles/nanographene functionalized nanocomposites-based thrombin electrochemiluminescence aptasensor

Chen, ChaDan; Wei, Guobing; Yao, Xiaoyu; Liao, Fang; Peng, Hong Mei; Zhang, Jing; Hong, Nian; Cheng, Lin; Fan, Hao

doi:10.1007/s10008-018-3910-6

Cited by 12 publications

(5 citation statements)

References 36 publications

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“…Furthermore, a comparative study was also conducted with other ECL aptasensors using Ru(bpy) 3 2 + and its derivatives as the luminescent substance for the detection of TB. As shown in Table 2 [11,14,15,24,28,[37][38][39][40], the proposed method performed comparable or even lower detection limit than other ECL aptasensors. It indicated that the combination of the PTG-AuNPs modified electrode and RuAg/SiO 2 NPs as signal-amplifying tags can effectively amplify the ECL signal and improve the sensitivity of the aptasensor, which makes the proposed sensing platform a reliable means of detection.…”

Section: Analytical Performance Of the Aptasensor To Tbmentioning

confidence: 81%

“…[Ru(phen) 2 (cpaphen)] 2 + /PEI CNTs-Nf/PEI@ DGNPs pNAMA-Ru-HGNP 1 fM-10 nM 0.54 fM [11] Ru(bpy) 3 2 + /TPrA Au/GO Ru-TBA II-AuNPs 0.01-10 nM 6.3 pM [14] Ru(bpy) 3 2 + /PEI Au/Ru-PEI-PAA Hemin/TBA/Au@ CeO 2 0.1 pM-10 nM 0.03 pM [28] Sandwich RuÀ SnS 2 /PAMAM Au@ZnO Au-PAMAM-RuÀ SnS 2 NP 5 fM-1 nM 2.6 fM [24] Ru(bpy) 3 2 + /Fc Ru(bpy) 3 2 + / β-cyclodextrinÀ Au/ GR Au/GR 0.4-1000 pM 0.23 pM [37] Ru(phen) 3…”

Section: Detection Limitmentioning

confidence: 99%

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A Sandwich‐type Electrochemiluminescence Aptasensor for Thrombin Based on Functional Co‐polymer Electrode Using Ru(bpy)₃²⁺ Doped Nanocomposites as Signal‐amplifying Tags

Shan

Gong

et al. 2019

Electroanalysis

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Herein, a signal‐on sandwich‐type electrochemiluminescence (ECL) aptasensor for the detection of thrombin (TB) was proposed. The graphene (GR) doped thionine (TH) was electropolymerized synchronously on the bare glassy carbon electrode (GCE) to form co‐polymer (PTG) electrode. The gold nanoparticles (AuNPs) were decorated on the surface of the PTG by in‐situ electrodeposition, and the functional co‐polymer (PTG‐AuNPs) electrode was utilized as sensing interface. Then, TB binding aptamer I (TBA I) as capture probes were modified on the PTG‐AuNPs electrode to capture TB, and Ru(bpy)32+/silver nanoparticles doped silica core‐shell nanocomposites‐labeled TB binding aptamer II (RuAg/SiO2NPs@TBA II) were used as signal probes to further bind TB, resulting in a sandwich structure. With the assistant of silica shell and AgNPs, the enrichment and luminous efficiency of Ru(bpy)32+ were significantly improved. Under the synergy of PTG‐AuNPs and RuAg/SiO2NPs, the ECL signal was dramatically increased. The proposed ECL aptasensor displayed a wide linear range from 2 fM to 2 pM with the detection limit of 1 fM, which is comparable or better than that in reported ECL aptasensors for TB using Ru(bpy)32+ and its derivatives as the luminescent substance. The excellent sensitivity makes the proposed aptasensor a promising potential in pharmaceutical and clinical analysis.

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Section: Analytical Performance Of the Aptasensor To Tbmentioning

confidence: 81%

Section: Detection Limitmentioning

confidence: 99%

A Sandwich‐type Electrochemiluminescence Aptasensor for Thrombin Based on Functional Co‐polymer Electrode Using Ru(bpy)₃²⁺ Doped Nanocomposites as Signal‐amplifying Tags

Shan

Gong

et al. 2019

Electroanalysis

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“…In a more recent work, Fan and coworkers developed a functionalized nanocomposite-based electrochemiluminescence (ECL) sensor for detecting thrombin [ 55 ]. In this study, Ru(bpy) 3 2+ /β-CD-AuNPs/nanographene (NGP) composites were used to modify the glassy carbon electrode (GCE) surface, and then aptamers (TBA1 and TBA2 with a 1:1 M ratio) were labeled with Fc to act as the probed and were attached to the composites via the host–guest recognition between β-CD and Fc.…”

Section: Self-assembly Of Cyclodextrin-coated Nanoparticlesmentioning

confidence: 99%

Self-Assembly of Cyclodextrin-Coated Nanoparticles:Fabrication of Functional Nanostructures for Sensing and Delivery

et al. 2023

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In recent years, the bottom-up approach has emerged as a powerful tool in the fabrication of functional nanomaterials through the self-assembly of nanoscale building blocks. The cues embedded at the molecular level provide a handle to control and direct the assembly of nano-objects to construct higher-order structures. Molecular recognition among the building blocks can assist their precise positioning in a predetermined manner to yield nano- and microstructures that may be difficult to obtain otherwise. A well-orchestrated combination of top-down fabrication and directed self-assembly-based bottom-up approach enables the realization of functional nanomaterial-based devices. Among the various available molecular recognition-based “host–guest” combinations, cyclodextrin-mediated interactions possess an attractive attribute that the interaction is driven in aqueous environments, such as in biological systems. Over the past decade, cyclodextrin-based specific host–guest interactions have been exploited to design and construct structural and functional nanomaterials based on cyclodextrin-coated metal nanoparticles. The focus of this review is to highlight recent advances in the self-assembly of cyclodextrin-coated metal nanoparticles driven by the specific host–guest interaction.

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“…Moreover, the alteration in the levels of TB is associated with leukemia, vascular wall inflammation, tumor growth, and metastasis [ 29 ]. As an illustration, it has a modulating effect on tumor cell proliferation by enhancing growth at a low concentration of cancer cells, preventing growth at a high concentration, and can also produce an apoptosis effect [ 30 ]. TB promotes tumor cell adherence to platelets, endothelial cells, and subendothelial matrix proteins.…”

Section: Introductionmentioning

confidence: 99%

Recent Progresses in Development of Biosensors for Thrombin Detection

et al. 2022

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Thrombin is a serine protease with an essential role in homeostasis and blood coagulation. During vascular injuries, thrombin is generated from prothrombin, a plasma protein, to polymerize fibrinogen molecules into fibrin filaments. Moreover, thrombin is a potent stimulant for platelet activation, which causes blood clots to prevent bleeding. The rapid and sensitive detection of thrombin is important in biological analysis and clinical diagnosis. Hence, various biosensors for thrombin measurement have been developed. Biosensors are devices that produce a quantifiable signal from biological interactions in proportion to the concentration of a target analyte. An aptasensor is a biosensor in which a DNA or RNA aptamer has been used as a biological recognition element and can identify target molecules with a high degree of sensitivity and affinity. Designed biosensors could provide effective methods for the highly selective and specific detection of thrombin. This review has attempted to provide an update of the various biosensors proposed in the literature, which have been designed for thrombin detection. According to their various transducers, the constructions and compositions, the performance, benefits, and restrictions of each are summarized and compared.

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Ru(bpy)32+/β-cyclodextrin-Au nanoparticles/nanographene functionalized nanocomposites-based thrombin electrochemiluminescence aptasensor

Cited by 12 publications

References 36 publications

A Sandwich‐type Electrochemiluminescence Aptasensor for Thrombin Based on Functional Co‐polymer Electrode Using Ru(bpy)₃²⁺ Doped Nanocomposites as Signal‐amplifying Tags

A Sandwich‐type Electrochemiluminescence Aptasensor for Thrombin Based on Functional Co‐polymer Electrode Using Ru(bpy)₃²⁺ Doped Nanocomposites as Signal‐amplifying Tags

Self-Assembly of Cyclodextrin-Coated Nanoparticles:Fabrication of Functional Nanostructures for Sensing and Delivery

Recent Progresses in Development of Biosensors for Thrombin Detection

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Ru(bpy)32+/β-cyclodextrin-Au nanoparticles/nanographene functionalized nanocomposites-based thrombin electrochemiluminescence aptasensor

Cited by 12 publications

References 36 publications

A Sandwich‐type Electrochemiluminescence Aptasensor for Thrombin Based on Functional Co‐polymer Electrode Using Ru(bpy)32+ Doped Nanocomposites as Signal‐amplifying Tags

A Sandwich‐type Electrochemiluminescence Aptasensor for Thrombin Based on Functional Co‐polymer Electrode Using Ru(bpy)32+ Doped Nanocomposites as Signal‐amplifying Tags

Self-Assembly of Cyclodextrin-Coated Nanoparticles:Fabrication of Functional Nanostructures for Sensing and Delivery

Recent Progresses in Development of Biosensors for Thrombin Detection

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A Sandwich‐type Electrochemiluminescence Aptasensor for Thrombin Based on Functional Co‐polymer Electrode Using Ru(bpy)₃²⁺ Doped Nanocomposites as Signal‐amplifying Tags

A Sandwich‐type Electrochemiluminescence Aptasensor for Thrombin Based on Functional Co‐polymer Electrode Using Ru(bpy)₃²⁺ Doped Nanocomposites as Signal‐amplifying Tags