Rudimentary Capillary−Electrode Alignment for Capillary Electrophoresis with Electrochemical Detection

Abstract

A capillary-electrode holder was constructed for electrochemical detection in capillary electrophoresis (CE). The device allows for positioning of the working electrode at the end of the capillary column without the aid of micropositioners or microscopes. The design facilitates the exchange of electrodes and capillaries without the need of refabricating the entire capillary-electrode setup. The system can be assembled in a very short period of time. Alignment with the self-guided system proved to be reproducible for the electrodes used (carbon, nickel, copper). The advantages of reduced downtime and low cost, make the device very attractive for the routine analysis of electroactive species by CE with electrochemical detection.

Development of a new method for analysis of Sudan dyes by pressurized CEC with amperometric detection

“…The pCEC-AD system was sensitive, low cost, and capable of preventing bubble formation and maintaining reproducibility of the pCEC performance. The end-column amperometric cell designed in our experiment was in accordance with previous reports [19], which eliminated the requirement for the precise alignment.…”

“…The AD was performed by using a home-made detection cell which was similar to previous reports [19] (see Fig. 2) with a self-adjusted electrode holder (see Fig.…”

A Handy Detection Cell for End-Column Electrochemical Detection in Capillary Electrophoresis

“…have appeared. [14][15][16][17][18][19][20][21][22][23][24][25][26] The applications of these capillaries in the end-column CE-ECD mode are attractive because problems with clogging or bubble entrapment within the detection zone are minimized. However, in most cases, the working electrode still has to be positioned by means of micropositioners and with the aid of a microscope or simply by following a trial and error procedure.…”

“…In order to overcome the above-mentioned problems, Femier et al have proposed a capillary-electrode holder which secures the separation capillary and the working electrode without the aid of either microscopes or micropositioners. 15 Tudos et al described a detection cell consisting of a tee, where a carbon fiber bundle disk electrode was juxtaposed with the capillary using PEEK tubing and fittings. 28 The third arm of the tee was used for the reference electrode.…”

Detecção eletroquímica em eletroforese capilar

“…Alguns autores têm proposto celas eletroquímicas e arranjos que dispensam etapas laboriosas de alinhamento [29][30][31][32][33] . A maneira mais eficiente de minimizar a interferência do campo elétrico utilizado na separação é através do emprego de desacopladores, como na detecção OffC.…”