Rules for functional microRNA targeting

Kim, Doyeon; Chang, Hee Ryung; Baek, Daehyun

doi:10.5483/bmbrep.2017.50.11.179

Cited by 35 publications

(25 citation statements)

References 55 publications

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“…MiRNA is a type of endogenous non-coding single-stranded RNA molecule of 18-22 nt in length that can degrade or inhibit translation by specific binding to the 3'UTR region of the target mRNA, and it also plays an important regulatory role in the biological processes of cell growth, differentiation, embryonic development and immune response [9]. According to previous reports [10][11][12], 2588 mature miRNAs have been identified in the human body, and each miRNA can widely participate in various life activities of the human body, and regulate the occurrence and development of diseases and cancers.…”

Section: Discussionmentioning

confidence: 99%

Mechanism of miR-143-3p inhibiting proliferation, migration and invasion of osteosarcoma cells by targeting MAPK7

Hou

Feng

Jiao

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Objective: To investigate the effects of miR-143-3p and MAPK7 on the proliferation, migration, and invasion of U2OS human osteosarcoma (OS) cells. Methods: The expression of miR-143-3p and MAPK7 in U2OS cells were detected by qRT-PCR, and the protein level of MAPK7 was measured by western blot assay. The targeting relationship between miR-143-3p and MAPK7 was predicted by TargetScan and verified by dual luciferase reporter assay. MTT and Transwell assays were used to detect cell viability, migrated cells and invaded cells of U2OS cells. Results: Compared with hFOB1.19 cells, the expression of miR-143-3p was down-regulated and MAPK7 was up-regulated in U2OS cells. Cell viability, migration and invasion ability significantly decreased induced by miR-143-3p overexpression or MAPK7 knockdown in U2OS cells. The results of dual luciferase reporter assay indicated that miR-143-3p interacted with MAPK7. Furthermore, overexpression of MAPK7 could reverse the inhibitory effects on cell proliferation, migration and invasion in U2OS cells induced by miR-143-3p mimics. Conclusion: miR-143-3p could inhibit proliferation, migration and invasion of U2OS cells by targeting MAPK7.

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Section: Discussionmentioning

confidence: 99%

Mechanism of miR-143-3p inhibiting proliferation, migration and invasion of osteosarcoma cells by targeting MAPK7

Hou

Feng

Jiao

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…us, it is critical to understand the molecular mechanisms of ESCA and explore reliable prognostic biomarkers. A large number of studies have reported that miRNAs could play a crucial role in cancer by functioning as oncogenes or tumor suppressor genes [18]. Moreover, it has been demonstrated that the miRNA profile of tissues exhibits characteristic changes in certain types of tumors [19,20].…”

Section: Discussionmentioning

confidence: 99%

A Novel Three-miRNA Signature Identified Using Bioinformatics Predicts Survival in Esophageal Carcinoma

Zhang

Dai

2020

BioMed Research International

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Objective. We identified differentially expressed microRNAs (DEMs) between esophageal carcinoma (ESCA) tissues and normal esophageal tissues. We then constructed a novel three-miRNA signature to predict the prognosis of ESCA patients using bioinformatics analysis. Materials and Methods. We combined two microarray profiling datasets from the Gene Expression Omnibus (GEO) database and RNA-seq datasets from the Cancer Genome Atlas (TCGA) database to analyze DEMs in ESCA. The clinical data from 168 ESCA patients were selected from the TCGA database to assess the prognostic role of the DEMs. The TargetScan, miRDB, miRWalk, and DIANA websites were used to predict the miRNA target genes. Functional enrichment analysis was conducted using the Database for Annotation, Visualization, and Integrated Discovery (David), and protein-protein interaction (PPI) networks were obtained using the Search Tool for the Retrieval of Interacting Genes database (STRING). Results. With cut-off criteria of P<0.05 and |log2FC| > 1.0, 33 overlapping DEMs, including 27 upregulated and 6 downregulated miRNAs, were identified from GEO microarray datasets and TCGA RNA-seq count datasets. The Kaplan–Meier survival analysis indicated that a three-miRNA signature (miR-1301-3p, miR-431-5p, and miR-769-5p) was significantly associated with the overall survival of ESCA patients. The results of univariate and multivariate Cox regression analysis showed that the three-miRNA signature was a potential prognostic factor in ESCA. Furthermore, the gene functional enrichment analysis revealed that the target genes of the three miRNAs participate in various cancer-related pathways, including viral carcinogenesis, forkhead box O (FoxO), vascular endothelial growth factor (VEGF), human epidermal growth factor receptor 2 (ErbB2), and mammalian target of rapamycin (mTOR) signaling pathways. In the PPI network, three target genes (MAPK1, RB1, and CLTC) with a high degree of connectivity were selected as hub genes. Conclusions. Our results revealed that a three-miRNA signature (miR-1301-3p, miR-431-5p, and miR-769-5p) is a potential novel prognostic biomarker for ESCA.

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“…The first miRNA genes known as lin-4 and let-7 and their role as post-transcriptional regulators of gene expression were described in Caenorhabditis elegans during the early 1990s [ 81 , 82 ]. Micro RNAs are small, ncRNA molecules of ~22 nucleotides in length that use short regions of complementarity of around six to eight nucleotides of their target mRNAs to repress gene expression [ 83 , 84 ]. Endogenous miRNA-complementary sites are located within the 3′ and 5′ untranslated regions (3′ and 5′ UTRs) of target mRNAs as well as in the coding sequence [ 85 , 86 , 87 ].…”

Section: Non-coding Rnasmentioning

confidence: 99%

Novel Roles of Non-Coding RNAs in Opioid Signaling and Cardioprotection

Melo

Ishida

Goldaraz

et al. 2018

ncRNA

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Cardiovascular disease (CVD) is a significant cause of morbidity and mortality across the world. A large proportion of CVD deaths are secondary to coronary artery disease (CAD) and myocardial infarction (MI). Even though prevention is the best strategy to reduce risk factors associated with MI, the use of cardioprotective interventions aimed at improving patient outcomes is of great interest. Opioid conditioning has been shown to be effective in reducing myocardial ischemia-reperfusion injury (IRI) and cardiomyocyte death. However, the molecular mechanisms behind these effects are under investigation and could provide the basis for the development of novel therapeutic approaches in the treatment of CVD. Non-coding RNAs (ncRNAs), which are functional RNA molecules that do not translate into proteins, are critical modulators of cardiac gene expression during heart development and disease. Moreover, ncRNAs such as microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) are known to be induced by opioid receptor activation and regulate opioid signaling pathways. Recent advances in experimental and computational tools have accelerated the discovery and functional characterization of ncRNAs. In this study, we review the current understanding of the role of ncRNAs in opioid signaling and opioid-induced cardioprotection.

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Rules for functional microRNA targeting

Cited by 35 publications

References 55 publications

Mechanism of miR-143-3p inhibiting proliferation, migration and invasion of osteosarcoma cells by targeting MAPK7

Mechanism of miR-143-3p inhibiting proliferation, migration and invasion of osteosarcoma cells by targeting MAPK7

A Novel Three-miRNA Signature Identified Using Bioinformatics Predicts Survival in Esophageal Carcinoma

Novel Roles of Non-Coding RNAs in Opioid Signaling and Cardioprotection

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