As a reflection of the ecological pressures associated with rapid modernization and globalization, the environment has become an enduring theme of public debate and protest in Latin America. Over the past decade, scholars have made increasing connections between such debate and a range of questions related to citizenship. Meanwhile, a discourse of 'environmental citizenship' has a growing prevalence in policy across the region. While these developments echo similar political and academic trends in the Global North, the Latin American context demands a unique set of theoretical and methodological approaches to studying the intersection of ecology and citizenship, sensitive to the specific historical, cultural, and ecological character of the region. We outline a research agenda spanning questions of land, identity and citizenship; environmental justice and de-colonization; social subjectivity and the state; urban natures and citizens; and the materiality/subjectivity of nature. This array of approaches points to a more acute conceptualization of citizenship, both in terms of its understanding of politics and its treatment of ecology; it also offers a point of view that recognizes citizens and natures as dynamic realities, which mutually condition each other in a sphere of ongoing contest.