Ruminococcus bromii, identification and isolation as a dominant community member in the rumen of cattle fed a barley diet

Klieve, Athol V.; O’Leary, M. N.; McMillen, L.; Ouwerkerk, D.

doi:10.1111/j.1365-2672.2007.03492.x

Cited by 56 publications

(58 citation statements)

References 26 publications

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“…All gels were run at 100 V and 60 1C over 18 h in 0.5 Â TAE buffer (Tris-acetate, 0.04 M; EDTA, 0.001 M). A reference ladder of Klieve et al (2007), consisting of amplicons from isolates with various melting profiles spanning the length of the gel, was run on all gels. DNA bands were visualized following electrophoresis by silver staining (Kocherginskaya et al, 2005).…”

Section: Dna Extraction Proceduresmentioning

confidence: 99%

Microbial ecology of the equine hindgut during oligofructose-induced laminitis

et al. 2008

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Alimentary carbohydrate overload is a significant cause of laminitis in horses and is correlated with drastic shifts in the composition of hindgut microbiota. Equine hindgut streptococcal species (EHSS), predominantly Streptococcus lutetiensis, have been shown to be the most common microorganisms culturable from the equine caecum prior to the onset of laminitis. However, the inherent biases of culture-based methods are estimated to preclude up to 70% of the normal caecal microbiota. The objective of this study was to evaluate bacterial population shifts occurring in the equine caecum throughout the course of oligofructose-induced laminitis using several cultureindependent techniques and to correlate these with caecal lactate, volatile fatty acid and degrees of polymerization 3-7 fructo-oligosaccharide concentrations. Our data conclusively show that of the total microbiota present in the equine hindgut, the EHSS S. lutetiensis is the predominant microorganism that proliferates prior to the onset of laminitis, utilizing oligofructose to produce large quantities of lactate. Population shifts in lactobacilli and Escherichia coli subpopulations occur secondarily to the EHSS population shifts, thus confirming that lactobacilli and coliforms have no role in laminitis. A large, curved, Gram-negative rod previously observed during the early phases of laminitis induction was most closely related to the Anaerovibrio genus and most likely represents a new, yet to be cultured, genus and species. Correlation of fluorescence in situ hybridization and quantitative real-time PCR results provide evidence supporting the hypothesis that laminitis is associated with the death en masse and rapid cell lysis of EHSS. If EHSS are lysed, liberated cellular components may initiate laminitis.

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Section: Dna Extraction Proceduresmentioning

confidence: 99%

Microbial ecology of the equine hindgut during oligofructose-induced laminitis

et al. 2008

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“…In line with this hypothesis, is the finding of three other genus-like (level 2) groups that co-occur with Oscillospira guillermondii et rel. (Figure 2c) which have isolates associated with degradation of plant material: Clostridium cellulosi (Yanling et al, 1991), Ruminococcus bromii (Klieve et al, 2007;Kovatcheva-Datchary et al, 2009) and Sporobacter termitidis (Grech-Mora et al, 1996). It seems that the co-occurrence network of Oscillospira guillermondii et rel.…”

Section: Uncultured Clostridiales I Uncultured Clostridiales Iimentioning

confidence: 99%

Microbiota conservation and BMI signatures in adult monozygotic twins

et al. 2012

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The human gastrointestinal (GI) tract microbiota acts like a virtual organ and is suggested to be of great importance in human energy balance and weight control. This study included 40 monozygotic (MZ) twin pairs to investigate the influence of the human genotype on GI microbiota structure as well as microbial signatures for differences in body mass index (BMI). Phylogenetic microarraying based on 16S rRNA genes demonstrated that MZ twins have more similar microbiotas compared with unrelated subjects (Po0.001), which allowed the identification of 35 genus-like microbial groups that are more conserved between MZ twins. Half of the twin pairs were selected on discordance in terms of BMI, which revealed an inverse correlation between Clostridium cluster IV diversity and BMI. Furthermore, relatives of Eubacterium ventriosum and Roseburia intestinalis were positively correlated to BMI differences, and relatives of Oscillospira guillermondii were negatively correlated to BMI differences. Lower BMI was associated with a more abundant network of primary fiber degraders, while a network of butyrate producers was more prominent in subjects with higher BMI. Combined with higher butyrate and valerate contents in the fecal matter of higher BMI subjects, the difference in microbial networks suggests a shift in fermentation patterns at the end of the colon, which could affect human energy homeostasis.

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“…Gene sequences were compared with GenBank, EMBL, and DBJJ non-redundant nucleotide databases using the gapped basic local alignment search tool database search program (Altschul et al 1990) at the National Centre for Biotechnology Information (Bethesda, MA, USA). Phylogenetic analysis followed the protocols described by Klieve et al (2007).…”

Section: Dna Sequencing and Analysismentioning

confidence: 99%

Diversity of methanogens in ruminants in Queensland

Ouwerkerk

Turner

Klieve

2008

Aust. J. Exp. Agric.

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Abstract. Methane emissions from ruminant livestock represent a loss of carbon during feed conversion, which has implications for both animal productivity and the environment because this gas is considered to be one of the more potent forms of greenhouses gases contributing to global warming. Many strategies to reduce emissions are targeting the methanogens that inhabit the rumen, but such an approach can only be successful if it targets all the major groups of ruminant methanogens. Therefore, a thorough knowledge of the diversity of these microbes in different breeds of cattle and sheep, as well as in response to different diets, is required. A study was undertaken using the molecular techniques denaturing gradient gel electrophoresis, DNA cloning and DNA sequence analysis to define the extent of diversity among methanogens in ruminants, particularly Bos indicus cross cattle, on differing forages in Queensland. It was found that the diversity of methanogens in forage-fed cattle in Queensland was greater than in grain-fed cattle but there was little variability in methanogen community composition between cattle fed different forages. The species that dominate the rumen microbial communities of B. indicus cross cattle are from the genus Methanobrevibacter, although rumen-fluid inoculated digestors fed Leucaena leucocephala leaf were populated with Methanosphaera-like strains, with the Methanobrevibacter-like strains displaced. If ruminant methane emissions are to be reduced, then antimethanogen bioactives that target both broad groups of ruminant methanogens are most likely to be needed, and as a part of an integrated suite of approaches that redirect rumen fermentation towards other more useful end products.

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Ruminococcus bromii, identification and isolation as a dominant community member in the rumen of cattle fed a barley diet

Cited by 56 publications

References 26 publications

Microbial ecology of the equine hindgut during oligofructose-induced laminitis

Microbial ecology of the equine hindgut during oligofructose-induced laminitis

Microbiota conservation and BMI signatures in adult monozygotic twins

Diversity of methanogens in ruminants in Queensland

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