Ruthenium polypyridyl peptide conjugates: membrane permeable probes for cellular imaging

Neugebauer, Ute; Pellegrin, Yann; Devocelle, Marc; Forster, Robert J.; Signac, William; Moran, Niamh; Keyes, Tia E.

doi:10.1039/b810403d

Cited by 142 publications

(152 citation statements)

References 23 publications

Supporting

Mentioning

143

Contrasting

Unclassified

Order By: Relevance

“…These molecules are strongly absorbing chromophores often used as antennas for indirect excitation of metal ions, where direct excitation of a metal is inefficient. Phenanthroline itself is known to inhibit metallopeptidases and its various metal complexes have been prepared and tested as bioprobes [24][25][26][27][28][29]. Here we present results based on the synthesis, behavior in the different solvents monitored by NMR spectroscopy, photophysical analysis and cellular imaging of novel metal complexes under low concentrations.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and evaluation of the europiumIII and zincII complexes as luminescent bioprobes in high content cell-imaging analysis

Sergeeva

Donnier-Maréchal

Vaz

et al. 2011

Journal of Inorganic Biochemistry

View full text Add to dashboard Cite

SummaryNovel phenanthroline derivatives and their europium(III) and zinc(II) complexes have been prepared in up to 92 %. In contrast to the stable zinc complexes, the europium compounds exhibit a strong luminescence in THF solution and complete dissociation of the complexes back to free ligands in DMSO. 1 H NMR studies of the Eu(III)-complexes 5 and 6 also confirmed the existence of different states depending on the solvent used. Moreover, it was found that the compound 5 is stable in EtOH-PBS solutions; here a strong signal in the emission spectra corresponding to the europium ion was detected. These metal complexes can be used as fluorescence markers for the diagnosis of esophageal squamous carcinoma (OE21) cells at low concentrations. Cell images were acquired using the compound 5, 7-9 as luminescent markers. The first images were taken already after 20 min incubation time at a very low concentration range (0.7-1.6 µM).Published as: Sergeeva, N. N.; Donnier-Marechal, M.; Vaz, G.; Davies, A. M.; Senge, M. O. (2011): Synthesis and evaluation of the europium III and zinc II complexes as luminescent bioprobes in high content cell-imaging analysis.

show abstract

Section: Introductionmentioning

confidence: 99%

Synthesis and evaluation of the europiumIII and zincII complexes as luminescent bioprobes in high content cell-imaging analysis

Sergeeva

Donnier-Maréchal

Vaz

et al. 2011

Journal of Inorganic Biochemistry

View full text Add to dashboard Cite

show abstract

“…As mentioned earlier, lifetime measurements overcome the disadvantages of the intensitybased measurements. Luminescence lifetime, for example, is an ideal parameter to measure in biological system where the exact concentration of dye after cellular uptake is difficult to determine and replicate accurately [171].…”

Section: Sensing Methodologiesmentioning

confidence: 99%

Indicators for optical oxygen sensors

Borisov¹,

Quaranta²,

Klimant³

2012

Advances in Chemical Bioanalysis

View full text Add to dashboard Cite

Continuous monitoring of oxygen concentration is of great importance in many different areas of research which range from medical applications to food packaging. In the last three decades, significant progress has been made in the field of optical sensing technology and this review will highlight the one inherent to the development of oxygen indicators. The first section outlines the bioanalytical fields in which optical oxygen sensors have been applied. The second section gives the reader a comprehensive summary of the existing oxygen indicators with a critical highlight on their photophysical and sensing properties. Altogether, this review is meant to give the potential user a guide to select the most suitable oxygen indicator for the particular application of interest.

show abstract

“…In support to these targeted delivery works, Keyes group exploited the capacity of Ru(II) polypyridyl peptide conjugates as probes for cell imaging. 54,55 Metallopeptides incorporating other CPPs such as Tat [47][48][49][50][51][52][53][54][55][56][57] , sC18 or Penetratin have also been described. For instance, Keppler group reported the conjugation of a Pt(IV) analogue of oxaliplatin prodrug to the Tat 47-57 peptide (YGRKKRRQRRR-NH 2 ).…”

Section: Peptide-based Delivery Of Metal Complexesmentioning

confidence: 99%

Peptide-mediated vectorization of metal complexes: conjugation strategies and biomedical applications

et al. 2016

View full text Add to dashboard Cite

The rich chemical and structural versatility of transition metal complexes provide numerous novel paths to be pursued in the design of molecules that exert particular chemical of physicochemical effects that could operate over specific biological targets.However, the poor cell permeability of metallodrugs represents an important barrier for their therapeutic use. The conjugation between metal complexes and a functional peptide vector can be regarded as a versatile and potential strategy to improve their bioavailability and accumulation inside cells, and the site selectivity of their effect. This perspective lies on reviewing the recent advances on the design of metallopeptide conjugates for biomedical applications. Additionally, we highlight the studies where this approach has been directed towards the incorporation of redox active metal centers in living organisms for modulating the cellular redox balance, as a tool with application in anticancer therapy.

show abstract

Ruthenium polypyridyl peptide conjugates: membrane permeable probes for cellular imaging

Cited by 142 publications

References 23 publications

Synthesis and evaluation of the europiumIII and zincII complexes as luminescent bioprobes in high content cell-imaging analysis

Synthesis and evaluation of the europiumIII and zincII complexes as luminescent bioprobes in high content cell-imaging analysis

Indicators for optical oxygen sensors

Peptide-mediated vectorization of metal complexes: conjugation strategies and biomedical applications

Contact Info

Product

Resources

About