S. boulardii Early Intervention Maintains Gut Microbiome Structure and Promotes Gut Mucosal Barrier Function in Early-Weaned Rats

Abstract

Early weaning leads to the disorder of the gut microbiome and gut mucosal barrier injury. Early intervention of gut microbiome colonization contributes to the development of the gut microbiome and gut function. The aim of this study was to explore the effects of Saccharomyces boulardii (S. boulardii) early intervention on the gut microbiome structure and gut mucosal barrier function of early-weaned rats. The results showed that S. boulardii early intervention improved growth performance along with a decrease in pathogenic bacteria, an increase in beneficial bacteria, a stable and complex microbiome, and a high level of microbial metabolism. Moreover, S. boulardii upregulated the mucosal barrier function including goblet cells and relative gene expression, tight junction, and sIgA level. Furthermore, S. boulardii suppressed the inflammatory response and promoted the anti-inflammatory response. Our study may provide a possible early intervention strategy for preventing an early weaning-induced disorder of the gut microbiome and loss of gut mucosal barrier function.

Exploring functional metabolites and proteomics biomarkers in late-preterm and natural-born pigs

“…α-AKG, an intermediate of the CAC derived from glutamine, has been proposed as the key factor determining glutamine dependence in cells ( 46 ). Studies have demonstrated the significant role of the gut microbiome in the development of newborns’ gut mucosal barriers ( 47 ). In preterm neonates, necrotizing enterocolitis (NEC) is primarily caused by intestinal barrier immaturity, as indicated by increased intestinal permeability ( 48 ).…”

Early-life Microbiome-Derived HCA Enhances Type 3 Immunity via FXR- dependent m6A RNA methylation

“…The detailed protocol was described in the ref [60]. Briefly, tissues were extracted with RIPA Lysis Buffer supplemented with 100× Protease Inhibitor Cocktail.…”