S-gene Dropout and False-negative Reverse Transcriptase-polymerase Chain Reaction Tests

Balaji, SM

doi:10.4103/ams.ams_2_22

Cited by 4 publications

(5 citation statements)

References 11 publications

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“…For example, mutation in the SARS-CoV-2 spike (S) gene in a region targeted by diagnostic reverse transcription-polymerase chain reaction (RT-PCR) leads to S-gene dropout, therefore the PCR fails, leading to a false-negative result. 91 …”

Section: Discussionmentioning

confidence: 99%

A Framework for Public Health Authorities to Evaluate Health Determinants for Wastewater-Based Epidemiology

Eaton

Coxon

Pattis

et al. 2022

Environ Health Perspect

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Background: Wastewater-based epidemiology (WBE) is rapidly developing as a powerful public health tool. It can provide information about a wide range of health determinants (HDs), including community exposure to environmental hazards, trends in consumption of licit and illicit substances, spread of infectious diseases, and general community health. As such, the list of possible candidate HDs for WBE is almost limitless. Consequently, a means to evaluate and prioritize suitable candidates for WBE is useful, particularly for public health authorities, who often face resource constraints. Objectives: We have developed a framework to assist public health authorities to decide what HDs may be appropriate for WBE and what biomarkers could be used. This commentary reflects the experience of the authors, who work at the interface of research and public health implementation. Discussion: To be suitable for WBE, a candidate HD should address a public health or scientific issue that would benefit from better understanding at the population level. For HDs where information on individual exposures or stratification by population subgroups is required, WBE is less suitable. Where other methodologies are already used to monitor the candidate HD, consideration must be given to whether WBE could provide better or complementary information to the current approach. An essential requirement of WBE is a biomarker specific for the candidate HD. A biomarker in this context refers to any human-excreted chemical or biological that could act as an indicator of consumption or exposure to an environmental hazard or of the human health state. Suitable biomarkers should meet several criteria outlined in this commentary, which requires background knowledge for both the biomarker and the HD. An evaluation tree summarizing key considerations for public health authorities when assessing the suitability of candidate HDs for WBE and an example evaluation are presented. https://doi.org/10.1289/EHP11115

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Section: Discussionmentioning

confidence: 99%

A Framework for Public Health Authorities to Evaluate Health Determinants for Wastewater-Based Epidemiology

Eaton

Coxon

Pattis

et al. 2022

Environ Health Perspect

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“…Among them, S gene seems to be more sensitive to mutations resulting in S-gene failure or dropout [50]. This indicates that the S gene is more likely to give false negative results when used as the only target for RT-PCR testing compared to other viral proteins [51]. Mutations in the S1 subunit seem to affect the interaction with human ACE2 leading to failure of immune response and vaccination effectiveness [49].…”

Section: Mourtzikou Et Almentioning

confidence: 99%

Correlation Between SARS-CoV-2 Positive Cases Admitted to a Tertiary Care Hospital in Greece and S-gene Mutations

Mourtzikou,

Stamouli,

Toka

et al. 2024

EJBIOMED

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The novel coronavirus SARS-CoV-2 is the cause of the COVID-19 pandemic which rapidly spread worldwide in early 2020. The severity of symptoms of COVID-19 disease, especially among the elderly and high-risk population groups, as well as the complications of the disease, forced the immediate implementation of preventive and protection measures. Moreover, vaccination programs were focused on cooping the virus transmission and spread of the virus in the general population, as well as on the immunization of the community against COVID-19 infection. The circulation of SARS-CoV-2 globally still appears and, as reported to FluNet, was around 10% by the end of February 2024, while the number of new cases and the number of new deaths have decreased. From December 2019, when the first outbreak of the virus was detected in Wuhan, China, until now, SARS-CoV-2 has undergone numerous mutations that have had a major impact on its pathogenesis during the COVID-19 pandemic. Among the SARS-CoV-2 variants and mutations, some are geographically identified, such as D614G, B.1.1.7 in the United Kingdom, B.1.1.28 in Brazil, CAL.20C in Southern California, B.1.351 in South Africa, while B.1.617 and B.1.1.529 have been reported worldwide. The main reason for the identification of SARS-CoV-2 variants and mutations is to investigate any correlation between new mutations and their impact on vaccine efficacy, despite the reported high vaccination rates of the vaccines that were implemented worldwide. The aim of this study is to investigate the correlations between SARS-CoV-2 S-gene target mutations with demographic characteristics, vaccination, hospitalization, and history of previous COVID-19 disease, in patients that were admitted to “Agios Panteleimon” General Hospital of Nikea, Piraeus, Greece, between August 2023 and January 2024.

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“…The Omicron variant has about 50 mutations, most of which are in the spike proteins, which leads to an S-gene target failure [ 66 ]. This led to increased false-negative RT-PCR test results due to a 69–70del mutation, mainly in the PCR tests with S-gene target failure.…”

Section: The Impact Of Genetic Variation On the Sars-cov-2 Genomementioning

confidence: 99%

Mutations of SARS-CoV-2 and their impact on disease diagnosis and severity

Alquraan

Alzoubi

Rababa'h

2023

Informatics in Medicine Unlocked

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S-gene Dropout and False-negative Reverse Transcriptase-polymerase Chain Reaction Tests

Cited by 4 publications

References 11 publications

A Framework for Public Health Authorities to Evaluate Health Determinants for Wastewater-Based Epidemiology

A Framework for Public Health Authorities to Evaluate Health Determinants for Wastewater-Based Epidemiology

Correlation Between SARS-CoV-2 Positive Cases Admitted to a Tertiary Care Hospital in Greece and S-gene Mutations

Mutations of SARS-CoV-2 and their impact on disease diagnosis and severity

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