S1 glycoprotein gene analysis of infectious bronchitis viruses isolated in Korea

Abstract

Fifteen isolates of Infectious bronchitis virus (IBV) were obtained from the kidney, trachea, and cecal tonsil of IB suspected chickens between 2001 and 2002 years in Korea. The S1 glycoprotein gene of IBV isolates were amplified by reverse transcriptase - polymerase chain reaction (RT-PCR) and analyzed by restriction fragment length polymorphism (RFLP) analysis. Fifteen Korean IBV isolates were classified into 4 groups by their RFLP patterns using restriction enzymes, HaeIII, BstYI, and XcmI. The RFLP patterns for 3, 1, and 1 of 15 isolates corresponded to the patterns of IBV Arkansas, Connecticut, and Massachusetts strains, respectively. Ten of 15 isolates generated unique KM91 RFLP pattern that was observed in the IBV KM 91 strain previously isolated in Korea. To confirm genetic diversity in the S1 genes of IBV isolates, viral RNAs of representative 9 of 15 IBV isolates were amplified, cloned, sequenced and compared with published sequences for non-Korean IBV strains. Korean IBV isolates showed amino acid sequence similarity between 61.8% (K446-01 and K161-02) and 96.1% (K281-01 and K210-02) with each other and they showed amino acid sequence similarity between 42.9% (K161-02 and GA980470) and 96.5% (K203-02 and KB8523) compared to non-Korean IBV strains. By phylogenetic tree analysis, Korean IBV field isolates were branched into five clusters in which 3 clusters were differentiated from non-Korean IBV strains. Especially, Korean IBV isolates K069-01, K507-01, K774-01 and K142-02 formed a separate cluster. It seems that IBVs continue to evolve and IBVs showing various genetic differences may cocirculate in Korea.

Isolation of avian infectious bronchitis coronavirus from domestic peafowl (Pavo cristatus) and teal (Anas)

“…Compared with pf/CH/LKQ3/03, tl/CH/LDT3/03 had codon insertions at nucleotide positions 67-69, 351-353 and 365-367, all of which are located in the S1 region. Both tl/CH/LDT3/03 and pf/CH/LKQ3/03 contained a spike glycoprotein cleavage recognition site, Arg-Arg-Phe-ArgArg, which is identical to that found in the IBV Beaudette, H120, Mass41 and KB8523 strains and some Korean IBV isolates (Cavanagh et al, 1986;Jackwood et al, 2001;Lee et al, 2004).…”

“…Compared with pf/CH/LKQ3/03, tl/CH/LDT3/03 had codon insertions at nucleotide positions 67-69, 351-353 and 365-367, all of which are located in the S1 region. Both tl/CH/LDT3/03 and pf/CH/LKQ3/03 contained a spike glycoprotein cleavage recognition site, Arg-Arg-Phe-ArgArg, which is identical to that found in the IBV Beaudette, H120, Mass41 and KB8523 strains and some Korean IBV isolates (Cavanagh et al, 1986;Jackwood et al, 2001;Lee et al, 2004).Composition of the S1 part of the S gene has been used widely to type and investigate serotypic variation in IBV at the molecular level (Lin et al, 1991). Correspondingly, comparisons were made between the predicted S1 part of S proteins of pf/CH/LKQ3/03 and tl/CH/LDT3/03 and those of 23 Chinese field IBV strains, six IBV vaccine strains and two strains of turkey coronaviruses.…”

Existence of Avian Infectious Bronchitis Virus with a European-Prevalent 4/91 Genotype in Japan

“…Of these, the S1 glycoprotein is associated with virus attachment and is a major target of the neutralizing antibodies in chickens [5,8], so serotypic evolution in IBV is associated primarily with the sequences of the S1 glycoprotein [10,18]. Therefore, recent genetic grouping of IBV has been performed mainly on the basis of the nucleotide sequences of the S1 glycoprotein gene [9,11,12,17,18,20].Previously, our phylogenetic analysis of mainly hypervariable (HVR) regions of S1 glycoprotein genes revealed that Japanese IBV strains could be classified into five genetic groups, one of which is indigenous to Japan and can not be placed within the existing groups in other countries [14].In the present report, to define the epidemiology or relationships among recent IBV isolates in Japan during 2003-2007, we used essentially the same procedure in an attempt to characterize eight Japanese IBV isolates from chickens.The IBV isolates were obtained from prefecture-based regional animal hygiene service centers in Japan (Table 1). Most of the IBV specimens were isolated by two or three passages using 10-day-old embryonated chicken eggs.…”

“…Of these, the S1 glycoprotein is associated with virus attachment and is a major target of the neutralizing antibodies in chickens [5,8], so serotypic evolution in IBV is associated primarily with the sequences of the S1 glycoprotein [10,18]. Therefore, recent genetic grouping of IBV has been performed mainly on the basis of the nucleotide sequences of the S1 glycoprotein gene [9,11,12,17,18,20].…”

A Novel Genotype of Avian Infectious Bronchitis Virus Isolated in Japan in 2009

“…, Yoshiko OOTANI 4) , Kazunori MURAYAMA 5) , Ayako KARINO 6) , Toshikazu INOUE 1) and Junko KAWAKAMI ABSTRACT. Avian infectious bronchitis viruses (IBVs) isolated from commercial layer flocks kept in Ibaraki Prefecture in 2009 were genetically and serologically characterized.…”