S100 A9 is expressed and secreted by the oviduct epithelium, interacts with gametes and affects parameters of human sperm capacitation in vitro

Massa, Estefanía; Prez, Gastón Matías; Zumoffen, Carlos; Morente, Carlos; Ghersevich, Sergio

doi:10.1002/jcb.29033

Cited by 8 publications

(3 citation statements)

References 62 publications

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“…The formation of MDA as a consequence of LPO can modify proteins through oxidation of their thiol groups and carbonylation, resulting in cellular dysfunction and activation of the apoptotic cascade [68]. In this context, the sperm proteins Midkine, Cathepsin L2, Prosaposin, S100 calcium binding protein A9, Annexin, Metalloproteinase inhibitor 2, and Nucleobindin-1 that are involved in processes, such as sperm ability to overcome the barriers formed by cumulus cells, binding to the zona pellucida, fertilization, and embryonic development showed low abundance in the spermatozoa of the first ejaculate, evidencing the incompetence of these spermatozoa in fulfilling their functions [69][70][71][72]. Midkine is a heparin-binding protein that interacts with glycosaminoglycans of the cumulus oophorus complex, facilitating the binding of sperm to the oocyte.…”

Section: Discussionmentioning

confidence: 99%

Oxidative stress associated with proteomic and fatty acid profiles of sperm from Nellore bulls at rest

Ramírez-López,

Barros,

Vidigal

et al. 2023

Biology of Reproduction

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Sexual rest is a transient condition, which compromises conception rates, characterized by large volumes of ejaculate with high percentages of dead sperm observed in bulls. The biochemical mechanisms leading to this ejaculate pattern are not fully understood. Six adult resting Nellore bulls were submitted to Breeding Soundness Evaluation by four consecutive semen collections through the electroejaculation method during a 30 min period. Each ejaculate had its semen phenotypic parameters; morphology and physical aspects were evaluated. To assess enzymatic activity (superoxide dismutase, catalase, and glutathione S-transferase), lipid peroxidation (concentrations of malondialdehyde and nitric oxide), fatty acid, and proteomic profile aliquots of spermatozoa from the first and fourth ejaculates were used. All sperm parameters differed between the first and fourth ejaculates. Spermatozoa from the first ejaculate showed lower enzymatic activity and a higher concentration of lipid peroxidation markers. Among the 19 identified fatty acids, 52.7% are polyunsaturated. Relative abundance analysis showed that C12:0 and C18:0 fatty acids differed between the first and fourth ejaculates, being the fourth ejaculate richer in spermatozoa. The proteomics analysis identified a total of 974 proteins in both sample groups (first and fourth ejaculates). The majority of identified proteins are related to cellular processes and signaling. Quantitative proteomics showed 36 differentially abundant proteins, 6 up-regulated proteins in the first ejaculate, and 30 up-regulated proteins in the fourth ejaculate. Spermatozoa from bulls at sexual rest have less antioxidant capacity, causing changes in their fatty acid composition and protein profile, which generates the observed sperm pattern and lower fertilization capacity.

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Section: Discussionmentioning

confidence: 99%

Oxidative stress associated with proteomic and fatty acid profiles of sperm from Nellore bulls at rest

Ramírez-López,

Barros,

Vidigal

et al. 2023

Biology of Reproduction

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“…Equal volume of conidial suspensions of B05.10 and the ΔBcSEC4 mutant were inoculated into 10% YEPD liquid medium for 1 day, and extracellular proteins were precipitated using acetone at 4 °C. The samples were digested, and identification of proteins was performed using a NanoLC-ESI-MS/MS system; the analysis was carried out by ProtTech (Suzhou, China) (Massa et al 2019).…”

Section: Extracellular Protein Detectionmentioning

confidence: 99%

The small GTPase BcSec4 is involved in conidiophore development, membrane integrity, and autophagy in Botrytis cinerea

et al. 2022

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Small GTPases of the Rab family play important roles in membrane trafficking and autophagy. Previous studies have shown that the small GTPase Sec4 is involved in vegetative growth, protein secretion, and pathogenicity of fungal phytopathogens. In this study, the Rab GTPase BcSec4, an ortholog of the yeast Sec4p protein, was characterized in Botrytis cinerea. The ΔBcSEC4 mutant produces many abnormal conidiophores; it is thus defective in conidiation and displays attenuated pathogenicity. Notably, the germination and penetration of conidia are unaffected in the ΔBcSEC4 mutant. Further studies showed that the secretion of extracellular proteins was altered in the ΔBcSEC4 mutant, and the mutant showed impaired autophagy and defects in membrane integrity. Taken together, these results suggest that BcSec4 is critical for conidiophore development, membrane integrity, pathogenicity, and autophagy.

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“…involved in processes such as the ability of sperm to overcome the barriers formed by cumulus cells, binding to the zona pellucida, fertilization and embryonic development showed low abundance in the spermatozoa of the first ejaculate, evidencing the incompetence of these spermatozoa in fulfilling their functions [47][48][49][50] . Midkine is a heparin-binding protein that interacts with the glycosaminoglycans of the cumulus-oophorus complex, facilitating the binding of sperm to the oocyte.…”

Section: Discussionmentioning

confidence: 99%

Association of sperm oxidative stress, proteomics and fatty acid profiles in nellore bulls at sexual rest

López¹

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Association of sperm oxidative stress, proteomics and fatty acid profiles in nellore bulls at sexual rest. Adviser: Simone Eliza Facioni Guimarães. This study was carried out with the objective of determining the effect of sexual rest on the oxidative status of spermatozoa from adult Nellore bulls and its effect on the fatty acid composition and protein profile. Six bulls were used, which were submitted to Breeding Soundness Evaluation and consecutive semen collection by the electroejaculation method. For each semen collection, the physical and morphological characteristics of the ejaculates were evaluated using conventional optical microscopy and phase contrast microscopy. After analyzing the semen qualitative parameters, the first and last ejaculate of each were centrifuged to separate the spermatozoa from the seminal plasma. From sperm aliquots, the enzymatic activity of Super Oxido Dismutase, Catalase and Glutathione S-Transferase was determined by colorimetric methods. The markers of lipid peroxidation, malondialdehyde and nitric oxide were also determined. Using gas chromatography, the fatty acids of sperm from the first and last ejaculate were identified. For proteomic analysis, total sperm proteins were extracted and precipitated in a 4% CHAPS solution, then quantified by the Bradford method and subsequently analyzed by monodimensional short-run electrophoresis and mass spectrometry (LC-MS/MS). Proteins were identified using PEAKS software version 7.0. Proteins were functionally classified by the RPSBLAST tool of BLAST version 2.13.0 into categories from the KOG database. For the label-free quantitative proteomics analysis, proteins identified in at least three technical replicates of each group with a fold-change greater than 1.5 times were included. Data were analyzed using GraphPad Prisma software version 9.1. Four semen collections were necessary so that the bulls at sexual rest presented sperm parameters compatible with reproductive fitness. Differences (p<0.05) were observed between all physical and morphological characteristics of the first and last ejaculate. A total of 974 proteins were identified, of which 735 were common between the two groups and 123 and 116 proteins were unique to spermatozoa from the first and last ejaculate, respectively. Functional classification analysis revealed that KOG categories with the highest number of classified proteins were Posttranslational modification, protein turnover, chaperones and Cytoskeleton and Signal transduction mechanisms. The protein abundance profile showed that 6 proteins were differentially abundant in spermatozoa from the first ejaculate and 30 proteins weredifferentially abundant in spermatozoa from the last ejaculate. In addition, gas chromatography analysis allowed the identification of 19 fatty acids, 47.3% of the saturated type and 52.7% of the unsaturated type. The C12:0 and C18:0 fatty acids showed difference between the two groups, being more abundant in the spermatozoa of the last ejaculate. The results indicate that oxidative stress may be related to sperm characteristics displayed by bulls at sexual rest, since the accumulation of by-products of lipid peroxidation can cause alterations in the metabolic pathways used by spermatozoa to generate energy used for motility and alter fluidity. of the membrane causing sperm abnormalities. Keywords: Bovine. Semen. Antioxidants. Lipids. Proteins.

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S100 A9 is expressed and secreted by the oviduct epithelium, interacts with gametes and affects parameters of human sperm capacitation in vitro

Cited by 8 publications

References 62 publications

Oxidative stress associated with proteomic and fatty acid profiles of sperm from Nellore bulls at rest

Oxidative stress associated with proteomic and fatty acid profiles of sperm from Nellore bulls at rest

The small GTPase BcSec4 is involved in conidiophore development, membrane integrity, and autophagy in Botrytis cinerea

Association of sperm oxidative stress, proteomics and fatty acid profiles in nellore bulls at sexual rest

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