S1P promotes migration, differentiation and immune regulatory activity in amniotic-fluid–derived stem cells

Romani, Rita; Manni, Giorgia; Donati, Chiara; Pirisinu, Irene; Bernacchioni, Caterina; Gargaro, Marco; Pirro, Matteo; Calvitti, Mario; Bagaglia, Francesco; Sahebkar, Amirhossein; Clerici, Graziano; Matino, Davide; Pomili, Giorgio; Renzo, Gian Carlo Di; Talesa, Vincenzo Nicola; Puccetti, Paolo; Fallarino, Francesca

doi:10.1016/j.ejphar.2018.06.005

Cited by 16 publications

(14 citation statements)

References 56 publications

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“…[34] Additionally, hypoxia stimulates AFSC differentiation and inhibits AFSC senescence. [34,35] The genomic stability and epigenetic fidelity of AFSCs are high. De Coppi et al [10] injected AFSCs into the hind leg muscles of nude mice and found no evidence of teratoma formation after 3 months.…”

Section: Biological Properties Of Afscsmentioning

confidence: 99%

Applications of human amniotic fluid stem cells in wound healing

Luo

Wang

et al. 2022

Chinese Medical Journal

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Complete wound regeneration preserves skin structure and physiological functions, including sensation and perception of stimuli, whereas incomplete wound regeneration results in fibrosis and scarring. Amniotic fluid stem cells (AFSCs) would be a kind of cell population with self-renewing and non-immunogenic ability that have a considerable role in wound generation. They are easy to harvest, culture, and store; moreover, they are non-tumorigenic and not subject to ethical restrictions. They can differentiate into different kinds of cells that replenish the skin, subcutaneous tissues, and accessory organs. Additionally, AFSCs independently produce paracrine effectors and secrete them in exosomes, thereby modulating local immune cell activity. They demonstrate anti-inflammatory and immunomodulatory properties, regulate the physicochemical microenvironment of the wound, and promote full wound regeneration. Thus, AFSCs are potential resources in stem cell therapy, especially in scar-free wound healing. This review describes the biological characteristics and clinical applications of AFSCs in treating wounds and provide new ideas for the treatment of wound healing.

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Section: Biological Properties Of Afscsmentioning

confidence: 99%

Applications of human amniotic fluid stem cells in wound healing

Luo

Wang

et al. 2022

Chinese Medical Journal

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“…Purified DCs were re-suspended at 1 × 10 6 /ml in fresh media in the presence or absence of LPS and L-kynurenine for a total of 36 h; supernatants were collected and analyzed for TGF-β by ELISA according to the manufacturer's instructions (R&D system). DCs treated with LPS with either LPS or L-kynurenine were analyzed by ELISA for IL-10 and TGF-β contents according to the manufacturer's instructions (R&D system) (16).…”

Section: Cytokine Productionmentioning

confidence: 99%

Pharmacologic Induction of Endotoxin Tolerance in Dendritic Cells by L-Kynurenine

et al. 2020

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Endotoxin tolerance aims at opposing hyperinflammatory responses to lipopolysaccharide (LPS) exposure. The aryl hydrocarbon receptor (AhR) participates in protection against LPS-mediated tissue damage, as it plays a necessary role in restraining the proinflammatory action of IL-1β and TNF-α while fostering the expression of protective TGF-β. TGF-β, in turn, promotes durable expression of the immune regulatory enzyme indoleamine 2,3-dioxygenase 1 (IDO1). IDO1 degrades L-tryptophan to L-kynurenine-an activating ligand for AhR-thus establishing a feed-forward loop. In this study, we further demonstrate that L-kynurenine also promotes the dissociation of the Src kinase-AhR cytosolic complex, leading to the activation of both genomic and non-genomic events in conventional dendritic cells (cDCs) primed with LPS. Specifically, the Src kinase, by phosphorylating the downstream target IDO1, triggers IDO1's signaling ability, which results in enhanced production of TGF-β, an event key to establishing full endotoxin tolerance. We demonstrated that exogenous L-kynurenine can substitute for the effects of continued or repeated LPS exposure and that the AhR-Src-IDO1 axis represents a critical step for the transition from endotoxin susceptibility to tolerance. Moreover, much like fully endotoxin-tolerant dendritic cells (DCs) (i.e., treated twice with LPS in vitro), DCs-treated once with LPS in vitro and then with kynurenine-confer resistance on naïve recipients to an otherwise lethal LPS challenge. This may have clinical implications under conditions in which pharmacologically induced onset of endotoxin tolerance is a therapeutically desirable event.

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“…This is likely to be driven by plasma membrane changes in the organization of lipid rafts, with the inhibition of cholesterol synthesis and uptake that suppresses GBM/GSC survival, leading to alterations in S1P receptor subtypes in lipid rafts [106] . S1P is also a significant regulator of the migration and phenotype of immune and stem cells, and is therefore likely to be an important aspect in GSC formation as well as the intercellular interactions of the tumour microenvironment [107] . S1P is also an important regulator of immune cell mitochondrial function and metabolism [108] .…”

Section: Ceramide S1p and Estrogenmentioning

confidence: 99%

Glioblastoma chemoresistance: roles of the mitochondrial melatonergic pathway

Anderson¹

2020

CDR

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Treatment-resistance is common in glioblastoma (GBM) and the glioblastoma stem-like cells (GSC) from which they arise. Current treatment options are generally regarded as very poor and this arises from a poor conceptualization of the biological underpinnings of GBM/GSC and of the plasticity that these cells are capable of utilizing in response to different treatments. A number of studies indicate melatonin to have utility in the management of GBM/GSC, both per se and when adjunctive to chemotherapy. Recent work shows melatonin to be produced in mitochondria, with the mitochondrial melatonergic pathway proposed to be a crucial factor in driving the wide array of changes in intra-and inter-cellular processes, as well as receptors that can be evident in the cells of the GBM/GSC microenvironment. Variations in the enzymatic conversion of N -acetylserotonin (NAS) to melatonin may be especially important in GSC, as NAS can activate the tyrosine receptor kinase B to increase GSC survival and proliferation. Consequently, variations in the NAS/melatonin ratio may have contrasting effects on GBM/GSC survival. It is proposed that mitochondrial communication across cell types in the tumour microenvironment is strongly driven by the need to carefully control the mitochondrial melatonergic pathways across cell types, with a number of intra-and inter-cellular processes occurring as a consequence of the need to carefully regulate the NAS/melatonin ratio. This better integrates previously disparate data on GBM/GSC as well as providing clear future research and treatment options.

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S1P promotes migration, differentiation and immune regulatory activity in amniotic-fluid–derived stem cells

Cited by 16 publications

References 56 publications

Applications of human amniotic fluid stem cells in wound healing

Applications of human amniotic fluid stem cells in wound healing

Pharmacologic Induction of Endotoxin Tolerance in Dendritic Cells by L-Kynurenine

Glioblastoma chemoresistance: roles of the mitochondrial melatonergic pathway

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