Saccharomyces cerevisiae cell surface display technology: Strategies for improvement and applications

Abstract

Microbial cell surface display technology provides a powerful platform for engineering proteins/peptides with enhanced properties. Compared to the classical intracellular and extracellular expression (secretion) systems, this technology avoids enzyme purification, substrate transport processes, and is an effective solution to enzyme instability. Saccharomyces cerevisiae is well suited to cell surface display as a common cell factory for the production of various fuels and chemicals, with the advantages of large cell size, being a Generally Regarded As Safe (GRAS) organism, and post-translational processing of secreted proteins. In this review, we describe various strategies for constructing modified S. cerevisiae using cell surface display technology and outline various applications of this technology in industrial processes, such as biofuels and chemical products, environmental pollution treatment, and immunization processes. The approaches for enhancing the efficiency of cell surface display are also discussed.

Yeast oral vaccines against infectious diseases

“…Candidate yeast oral vaccines with cell surface display are being developed against a diverse array of pathogens, including White Spot Syndrome Virus, H5N1 Influenza A Virus, African Swine Fever Virus, and SARS-CoV2, among others, that infect a wide range of animal organisms including shrimp, fish, fowl, swine, and human beings ( Table 1 ). Most of these yeast oral vaccines use the a-agglutinin Aga1p-Aga2p Yeast Surface Display (YSD) system in S. cerevisiae ( Mei et al, 2017 ; Zhang C. et al, 2022 ). Here, the heterologous protein of interest, in our case, the candidate antigen/immunogen, is expressed as a fusion to the Aga2p protein, which in vivo is linked to cell-wall covalently-associated Aga1p through two disulfide bonds.…”

“…Here, the heterologous protein of interest, in our case, the candidate antigen/immunogen, is expressed as a fusion to the Aga2p protein, which in vivo is linked to cell-wall covalently-associated Aga1p through two disulfide bonds. A second system uses the alpha-agglutinin Sag1p to anchor the heterologous protein of interest directly to the cell wall ( Zhang C. et al, 2022 ).…”

Boosting sugarcane trash decomposition: synergistic action and proximity effect of xylanase and feruloyl esterase co-displayed on the cell surface of Pichia pastoris (Komagataella phaffi)

“…24 Thus, YSD offers several potential advantages over enzyme secretion for applications in lignocellulosic biomass degradation. 24 Immobilization on the cell surface can enhance enzyme stability and prevent degradation compared to free enzymes in solution. 25,26 Concentration of enzymes on the cell surface can result in higher local enzyme concentrations, thereby increasing catalytic efficiency.…”

“…However, it is crucial to note that in the context of a cell factory system, YSD offers unique practical benefits such as reusability and streamlined downstream purification, underscoring its potential in scaled bioprocessing and compound production. 24 Thus, YSD offers several potential advantages over enzyme secretion for applications in lignocellulosic biomass degradation. 24 Immobilization on the cell surface can enhance enzyme stability and prevent degradation compared to free enzymes in solution.…”

Extracellular Poly(hydroxybutyrate) Bioplastic Production Using Surface Display Techniques

“…More recently, biocatalysis applications for surface display have been demonstrated, for example, in microbial fermentation and fuel cell chemistry . Compared to enzymatic secretion, surface display offers many advantages including enhanced stability in varying temperature and pH, as well as increasing protein–protein interactions and substrate transfer . Surface display synthesis of materials is not yet well-studied, but the potential of enzymatic surface display systems in biocatalysis opens opportunities for extracellular synthesis of complex molecules like polymers.…”