Safe, Effective, and Inexpensive Clearance of
            <i>Mycoplasma</i>
            Contamination from Cultures of Apicomplexan Parasites with Sparfloxacin

Malave-Ramos, Darlene R; Kennedy, Kit; Key, Melanie; Dou, Zhicheng; Kafsack, Björn F.C.

doi:10.1128/spectrum.03497-22

Cited by 2 publications

(1 citation statement)

References 22 publications

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“…Contaminants such as Mycoplasma usually remain inconspicuous in continuous Plasmodium cultures but substantially alter the property and behavior of infected cells making them potential significant confounders in experimental studies ( Malave-Ramos et al, 2022 ). There is the prospect of repurposing the off-target property of Cas13 for detecting contaminants in long-term in vitro Plasmodium cell cultures ( Figure 5E ).…”

Section: Gene Manipulating Tools At the Rna Level In Plasmo...mentioning

confidence: 99%

CRISPR-Cas13 in malaria parasite: Diagnosis and prospective gene function identification

et al. 2023

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Malaria caused by Plasmodium is still a serious public health problem. Genomic editing is essential to understand parasite biology, elucidate mechanical pathways, uncover gene functions, identify novel therapeutic targets, and develop clinical diagnostic tools. Recent advances have seen the development of genomic diagnostic technologies and the emergence of genetic manipulation toolbox comprising a host of several systems for editing the genome of Plasmodium at the DNA, RNA, and protein level. Genomic manipulation at the RNA level is critical as it allows for the functional characterization of several transcripts. Of notice, some developed artificial RNA genome editing tools hinge on the endogenous RNA interference system of Plasmodium. However, Plasmodium lacks a robust RNAi machinery, hampering the progress of these editing tools. CRISPR-Cas13, which belongs to the VI type of the CRISPR system, can specifically bind and cut RNA under the guidance of crRNA, with no or minimal permanent genetic scar on genes. This review summarizes CRISPR-Cas13 system from its discovery, classification, principle of action, and diagnostic platforms. Further, it discusses the application prospects of Cas13-based systems in Plasmodium and highlights its advantages and drawbacks.

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Section: Gene Manipulating Tools At the Rna Level In Plasmo...mentioning

confidence: 99%

CRISPR-Cas13 in malaria parasite: Diagnosis and prospective gene function identification

et al. 2023

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Mycoplasma: Properties, Detection, and Decontamination Methods of Cell Cultures and Viral Strains (Review)

Leonovich

2024

Appl Biochem Microbiol

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Safe, Effective, and Inexpensive Clearance of Mycoplasma Contamination from Cultures of Apicomplexan Parasites with Sparfloxacin

Abstract: These findings will be useful to laboratories maintaining apicomplexan parasites in vitro , especially in low-resource environments, where the high cost of commercial products creates an economic barrier for detecting and eliminating mycoplasma from culture.

Cited by 2 publications

References 22 publications

CRISPR-Cas13 in malaria parasite: Diagnosis and prospective gene function identification

CRISPR-Cas13 in malaria parasite: Diagnosis and prospective gene function identification

Mycoplasma: Properties, Detection, and Decontamination Methods of Cell Cultures and Viral Strains (Review)

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