Safety Assessment of Graphene-Based Materials: Focus on Human Health and the Environment

Fadeel, Bengt; Bussy, Cyrill; Merino, Sonia; Vázquez, Éster; Flahaut, Emmanuel; Mouchet, Florence; Evariste, Lauris; Gauthier, Laury; Koivisto, Antti Joonas; Vogel, Ulla; Martín, Cristina; Delogu, Lucia Gemma; Buerki-Thurnherr, Tina; Wick, Peter; Beloin-Saint-Pierre, Didier; Hischier, Roland; Pelin, Marco; Carniel, Fabio Candotto; Tretiach, Mauro; Cesca, Fabrizia; Benfenati, Fabio; Scaini, Denis; Ballerini, Laura; Kostarelos, Kostas; Prato, Maurizio; Bianco, Alberto

doi:10.1021/acsnano.8b04758

Cited by 481 publications

(434 citation statements)

References 348 publications

Supporting

Mentioning

423

Contrasting

Unclassified

Order By: Relevance

“…In this context, the question about toxicity and immune compatibility becomes central for future nanomedical applications of graphene‐based materials including GO, which has been addressed in an increasing amount of studies . When injected into the blood stream, or administered through the airway, acute GO exposure at high doses can cause pulmonary inflammation, cardiovascular and systemic toxicity in mice and rats . GO can also adversely affect fecundity; some studies report toxicity to germ cells and reduced fertility in male mice that might depend on elevated cellular reactive oxygen species contents and subsequent DNA damage induction that in turn triggers apoptosis .…”

Section: Introductionmentioning

confidence: 99%

Improved Biocompatibility of Amino‐Functionalized Graphene Oxide in Caenorhabditis elegans

Rive

Reina

Wagle

et al. 2019

Small

Self Cite

View full text Add to dashboard Cite

Graphene oxide (GO) holds high promise for diagnostic and therapeutic applications in nanomedicine but reportedly displays immunotoxicity, underlining the need for developing functionalized GO with improved biocompatibility. This study describes adverse effects of GO and amino‐functionalized GO (GONH2) during Caenorhabditis elegans development and ageing upon acute or chronic exposure. Chronic GO treatment throughout the C. elegans development causes decreased fecundity and a reduction of animal size, while acute treatment does not lead to any measurable physiological decline. However, RNA‐Sequencing data reveal that acute GO exposure induces innate immune gene expression. The p38 MAP kinase, PMK‐1, which is a well‐established master regulator of innate immunity, protects C. elegans from chronic GO toxicity, as pmk‐1 mutants show reduced tissue‐functionality and facultative vivipary. In a direct comparison, GONH2 exposure does not cause detrimental effects in the wild type or in pmk‐1 mutants, and the innate immune response is considerably less pronounced. This work establishes enhanced biocompatibility of amino‐functionalized GO in a whole‐organism, emphasizing its potential as a biomedical nanomaterial.

show abstract

Section: Introductionmentioning

confidence: 99%

Improved Biocompatibility of Amino‐Functionalized Graphene Oxide in Caenorhabditis elegans

Rive

Reina

Wagle

et al. 2019

Small

Self Cite

View full text Add to dashboard Cite

show abstract

“…Prior to focus on these biological effects, scientists should carefully characterize the materials [40]. It has been reported that the number of graphene layers, the average lateral size and the carbon-to-oxygen (C/O) atomic ratio play key role in determining the biological effects [13].…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

“…Due to these interactions, the safety and toxicity of GBMs have been extensively evaluated in vitro and in vivo in various studies. In a recent review by Fadeel et al, it has been emphasized that these different properties of GBMs govern their biological effects and scientists should not consider graphene as a single material but rather focus on each individual GBM separately in order to better understand the structure-activity relationships [13].…”

Section: Introductionmentioning

confidence: 99%

A closer look at the genotoxicity of graphene based materials

et al. 2019

Self Cite

View full text Add to dashboard Cite

Graphene-based materials (GBMs) have attracted many scientists because of their optical, thermal, mechanical and electronic properties. Their good dispersibility in different type of solvents including water, the possibility to formulate them according to desired function, and the wide surface area, which can allow various chemical modifications, expanded the use of these materials in biological systems. For these reasons, GBMs have been extensively studied in vitro and in vivo in the biomedical field. However, the toxicity and genotoxicity of GBMs must be thoroughly investigated before they can be translated into clinical settings. The main mechanism of graphene toxicity is thought to be caused by reactive oxygen species produced in cells, which in turn interact with various biomolecules including DNA. In this review we aimed to discuss different genotoxicity studies performed with GBMs with specific focus on the different cell types and conditions. By comparing and discussing such reports, scientists will be able to engineer non toxic GBMs for future preclinical and/or clinical studies. In order to allow a safer and faster transition to clinic, future studies should involve state-ofthe-art technologies such as systems biology approaches or three-dimensional microfluidic systems, which can better predict the normal physiological scenario.

show abstract

“…[10][11][12][13][14][15][16][17] Moreover,i tp rovidest echniques for safe handling and methods for minimizing the toxicityo fn anoscale carriers for in vivo application. [18][19][20][21] These nanoscale carriers can significantly improve the bioavailability of ad rug and decrease the dose of administration, thereby enhancing its therapeutic efficacy with lowers ide effects. The anti-inflammatory activity of NSAIDs can compatibility,b ut size, shape, agglomeration state, and toxicity (presence of contaminants) can cause undesired inflammation.…”

Section: Introductionmentioning

confidence: 99%

Sonochemical Formation of Copper/Iron‐Modified Graphene Oxide Nanocomposites for Ketorolac Delivery

Radziuk

Mikhnavets

Vorokhta

et al. 2019

Chemistry A European J

View full text Add to dashboard Cite

Af easible sonochemicala pproach is described for the preparationo fc opper/iron-modified graphene oxide nanocomposites through ultrasonication (20 kHz, 18 Wcm À2 ) of an aqueous solutionc ontaining coppera nd iron ion precursors. Uniquec opper-, copper/iron-and iron-modified graphene oxide nanocomposites have as ubmicron size that is smaller than that of pristine GO and ah ighers urfacea rea enriched with Cu 2 O, CuO, and Fe 2 O 3 of multiform phases (a-, b-, e-, or g), FeO(OH), and sulfur-o rc arbon-containingc om-pounds. These nanocomposites are sonochemically intercalated with the nonsteroidal anti-inflammatory drug ketorolac, which results in the formationo fn anoscale carriers. Ketorolac monotonically disintegratesf rom these nanoscale carriers in aqueous solution upon adjustment of the pH from 1t o8 .T he disintegration of ketorolacp roceeds at a slowerr ate from the copper/iron-modified grapheneo xide at increased pH, but at af aster rate from the iron-modified graphene oxide undera cidic conditions.

show abstract

Safety Assessment of Graphene-Based Materials: Focus on Human Health and the Environment

Cited by 481 publications

References 348 publications

Improved Biocompatibility of Amino‐Functionalized Graphene Oxide in Caenorhabditis elegans

Improved Biocompatibility of Amino‐Functionalized Graphene Oxide in Caenorhabditis elegans

A closer look at the genotoxicity of graphene based materials

Sonochemical Formation of Copper/Iron‐Modified Graphene Oxide Nanocomposites for Ketorolac Delivery

Contact Info

Product

Resources

About