Safety Evaluation by Phenotypic and Genomic Characterization of Four Lactobacilli Strains with Probiotic Properties

Lee, Yerim; Bang, Won Yeong; Baek, Kwang-Rim; Kim, Geun-Hyung; Kang, Moon-Jin; Yang, Jungwoo; Seo, Seung-Oh

doi:10.3390/microorganisms10112218

Cited by 7 publications

(3 citation statements)

References 28 publications

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“…Whereas B. longum NK46 produced 0.4 ± 0.1 mM (2.2 ± 0.4%) and 19.2 ± 0.5 mM (97.8 ± 0.4%) of D(−)-lactate and L(+)-lactate, respectively. These results are consistent with those of previous studies [51,52]. According to Lee et al [51], some strains of Lactobacilli produced D(−)-lactate within the range of 31.4%-36.1%, which was lower than that of L(+)-lactate (63.9-68.6%).…”

Section: Lactate Production Of the Probiotic Strainssupporting

confidence: 93%

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The Probiotic Properties and Safety of Limosilactobacillus mucosae NK41 and Bifidobacterium longum NK46

Lee,

Jo,

Seo

et al. 2024

Microorganisms

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Probiotics should possess specific properties to exert beneficial effects, and their safety must be ensured for human consumption. The purpose of this study was to evaluate the probiotic properties and safety of Limosilactobacillus mucosae NK41 and Bifidobacterium longum NK46 isolated from human feces in vitro. Both strains exhibited high resistance to simulated gastrointestinal fluid. Furthermore, probiotic-related cell surface characteristics including auto-aggregation and cell surface hydrophobicity were assessed by measuring the absorbance at a wavelength of 600 nm, which demonstrated good auto-aggregation ability and affinity for xylene, indicating their effective adhesion to Caco-2 cells. In addition, hemolytic, gelatinase, and β-glucuronidase activities were found to be negative in both strains. The susceptibility to nine commonly used antibiotics was assessed using the broth macrodilution method, which demonstrated that both strains were susceptible to all tested antibiotics. Furthermore, L. mucosae NK41 and B. longum NK46 produced significantly higher levels of L-lactate (71.8 ± 0.7% and 97.8 ± 0.4%) than D-lactate (28.2 ± 0.7% and 2.2 ± 0.4%, respectively). Using PCR amplification to investigate genes associated with virulence factors, we found that neither strain harbored any virulence genes. These findings suggest that L. mucosae NK41 and B. longum NK46 have the potential to be used as probiotics and are considered safe for human consumption.

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Section: Lactate Production Of the Probiotic Strainssupporting

confidence: 93%

“…These results are consistent with those of previous studies [51,52]. According to Lee et al [51], some strains of Lactobacilli produced D(−)-lactate within the range of 31.4%-36.1%, which was lower than that of L(+)-lactate (63.9-68.6%). B. longum subsp.…”

Section: Lactate Production Of the Probiotic Strainssupporting

confidence: 93%

The Probiotic Properties and Safety of Limosilactobacillus mucosae NK41 and Bifidobacterium longum NK46

Lee,

Jo,

Seo

et al. 2024

Microorganisms

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“…salivarius CRL 1417 and E. faecium CRL 1385 adhered to a lesser degree (>9 and 2%, respectively) ( Huligere et al, 2023 ). In the study by Lee et al (2022) reported that Lactobacillus spp. Obtained in their study had good adhesion rate toward oral epithelial adhesion.…”

Section: Resultsmentioning

confidence: 99%

Investigating the antidiabetic efficacy of dairy-derived Lacticaseibacillus paracasei probiotic strains: modulating α-amylase and α-glucosidase enzyme functions

Huligere,

Kumari V B,

Desai

et al. 2023

Front. Microbiol.

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The current study aims to evaluate and characterize the probiotic andantidiabetic properties of lactic acid bacteria (LAB) obtained from milk and other dairy-based products. The strains were tested physiologically, biochemically, and molecularly. Based on biochemical tests and 16S rRNA gene amplification and sequencing, all three isolates RAMULAB18, RAMULAB19, and RAMULAB53 were identified as Lacticaseibacillus paracasei with homology similarity of more than 98%. The inhibitory potential of each isolate against carbohydrate hydrolysis enzymes (α-amylase and α-glucosidase) was assessed using three different preparations of RAMULAB (RL) isolates: the supernatant (RL-CS), intact cells (RL-IC), and cell-free extraction (RL-CE). Additionally, the isolate was evaluated for its antioxidant activity against free radicals (DPPH and ABTS). The strain’s RL-CS, RL-CE, and RL-IC inhibited α-amylase (17.25 to 55.42%), α-glucosidase (15.08–59.55%), DPPH (56.42–87.45%), and ABTS (46.35–78.45%) enzymes differently. With the highest survival rate (>98%) toward tolerance to gastrointestinal conditions, hydrophobicity (>42.18%), aggregation (>74.21%), as well as attachment to an individual’s colorectal cancer cell line (HT-29) (>64.98%), human buccal and chicken crop epithelial cells, all three isolates exhibited extensive results. All three isolates exhibited high resistance toward antibiotics (methicillin, kanamycin, cefixime, and vancomycin), and other assays such as antibacterial, DNase, hemolytic, and gelatinase were performed for safety assessment. Results suggest that the LAB described are valuable candidates for their significant health benefits and that they can also be utilized as a beginning or bio-preservative tradition in the food, agriculture, and pharmaceutical sectors. The LAB isolates are excellent in vitro probiotic applicants and yet additional in vivo testing is required.

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In vitro safety assessment of electrohydrodynamically encapsulated Lactiplantibacillus plantarum CRD7 and Lacticaseibacillus rhamnosus CRD11 for probiotics use

Varada¹,

Panneerselvam²,

Pushpadass³

et al. 2023

Current Research in Food Science

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Safety Evaluation by Phenotypic and Genomic Characterization of Four Lactobacilli Strains with Probiotic Properties

Cited by 7 publications

References 28 publications

The Probiotic Properties and Safety of Limosilactobacillus mucosae NK41 and Bifidobacterium longum NK46

The Probiotic Properties and Safety of Limosilactobacillus mucosae NK41 and Bifidobacterium longum NK46

Investigating the antidiabetic efficacy of dairy-derived Lacticaseibacillus paracasei probiotic strains: modulating α-amylase and α-glucosidase enzyme functions

In vitro safety assessment of electrohydrodynamically encapsulated Lactiplantibacillus plantarum CRD7 and Lacticaseibacillus rhamnosus CRD11 for probiotics use

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