Safety evaluation of arachidonic acid rich Mortierella alpina biomass in albino rats—A subchronic study

Nisha, A.; Muthukumar, S. P.; Venkateswaran, G.

doi:10.1016/j.yrtph.2009.01.002

Cited by 41 publications

(19 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, in the 90-day feeding study, body weight in females of RAO treatment groups was higher than in standard diet control group after 8 weeks. It was reported earlier that an effect of ARA on body weight during 2-4 weeks was observed (Nisha et al, 2009). In addition, 5 day old piglets supplemented for 2 weeks with ARA exhibited a 27% increase in body weight (Merritt et al, 2003).…”

Section: Parametersmentioning

confidence: 77%

“…Although some ARA products derived from other strains of M. alpina (1S-4 and I 49 -N 48 ) have proven safe Casterton et al, 2009;Tyburczy et al, 2011;Nisha et al, 2009), the safety of RAO (up to 48.3% ARA) derived from M. alpina XM027 has not been evaluated. Differences in the manufacturing process also may impact the safety of the final product (Mercer and Armenta, 2011).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A subchronic toxicity study, preceded by an in utero exposure phase, with refined arachidonic acid-rich oil (RAO) derived from Mortierella alpina XM027 in rats

Gao

Kang

et al. 2014

Regulatory Toxicology and Pharmacology

View full text Add to dashboard Cite

Section: Parametersmentioning

confidence: 77%

Section: Introductionmentioning

confidence: 99%

A subchronic toxicity study, preceded by an in utero exposure phase, with refined arachidonic acid-rich oil (RAO) derived from Mortierella alpina XM027 in rats

Gao

Kang

et al. 2014

Regulatory Toxicology and Pharmacology

View full text Add to dashboard Cite

“…M. alpina cultures were maintained on potato dextrose agar slants at 4°C and subcultured every 2 months [11]. The seed culture was prepared in 50 mL medium containing (g/L) glucose, 20; yeast extract, 10; the pH was adjusted to 6.0 and cultures were incubated for 48 h at 28°C.…”

Section: Methodsmentioning

confidence: 99%

“…Harvest and extraction of lipids from biomass were performed according to the procedure of Nisha et al [11]. Biomass production was determined by harvesting the cells by suction filtration followed by drying at 55–60°C overnight.…”

Section: Methodsmentioning

confidence: 99%

Production and Enhancement of Omega-3 Fatty Acid fromMortierella alpinaCFR-GV15: Its Food and Therapeutic Application

Vadivelan

Venkateswaran

2014

BioMed Research International

View full text Add to dashboard Cite

Mortierella sp. has been known to produce polyunsaturated fatty acids (PUFAs) such as GLA and AA under normal growth medium conditions. Similarly, under the stress condition, this fungus produces EPA and DHA in their mycelial biomass. Among the 67 soil samples screened from the Western Ghats of India, 11 Mortierella isolates showed the presence of omega-6 and omega-3 fatty acid, mainly GLA, AA, EPA, and DHA in starch, yeast-extract medium. Nile red and TTC strains were used for screening their qualitative oleaginesity. Among the representative isolates, when Mortierella sp. is grown in a fat-producing basal medium, a maximum lipid content of 42.0 ± 1.32% in its mycelia, 6.72 ± 0.5% EPA, and 4.09 ± 0.1% DHA was obtained. To understand the Mortierella sp. CFR-GV15, to the species level, its morphology was seen under the light microscope and scanning electron microscope, respectively. These microscopic observations showed that isolate Mortierella sp. CFR-GV15 produced coenocytic hyphae. Later on, its 18S rRNA and the internal transcribed spacer (ITS) sequences were cloned, sequenced, and analyzed phylogenetically to 18S rRNA and ITS1 and ITS4 sequences of related fungi. This newly isolated Mortierella alpina CFR-GV15 was found to be promising culture for the development of an economical method for commercial production of omega-3 fatty acid for food and therapeutical application.

show abstract

“…Many species of the genus Mortierella have been found to yield exceptionally high quantities of ARA depending on the fermentation media and culture conditions [7]. For the past few decades, research efforts have focused on further process development and scale-up of arachidonic acid production by M. alpina.…”

Section: Introductionmentioning

confidence: 99%

Optimization of media components for enhanced arachidonic acid production by Mortierella alpina under submerged cultivation

Nisha

Rastogi

Venkateswaran

2011

Biotechnol Bioproc E

View full text Add to dashboard Cite

Mortierella alpina, an oleaginous zygomycete is a potent producer of arachidonic acid, the pharmaceutically and nutraceutically important polyunsaturated fatty acid of the n-6 series. It serves a wide variety of purposes, from being a purely structural element in phospholipids to being involved in signal transduction, and as a substrate for a host of derivatives involved in second messenger function. Arachidonic acid has applications in diverse areas including infant and geriatric nutrition. In the present study, the interactive effects of four major media constituents on arachidonic acid production were investigated by applying a central composite rotatable design (CCRD) and response surface methodology (RSM). The independent variables, which were selected byconcentrations of glucose, corn solids, KH 2 PO 4 , and KNO 3 influenced the production of biomass, total lipid, and arachidonic acid by M. alpina. A second-order polynomial was fitted by multiple regression analysis of the experimental data. The optimum conditions (glucose 10.0 g/L, corn solids 5.0 g/L, KH 2 PO 4 1.0 g/L, and KNO 3 1.0 g/L) resulted in maximum production of arachidonic acid (1.39 g/L) and the corresponding biomass and total lipid concentrations were 12.49 and 5.87 g/L, respectively.

show abstract

Safety evaluation of arachidonic acid rich Mortierella alpina biomass in albino rats—A subchronic study

Cited by 41 publications

References 33 publications

A subchronic toxicity study, preceded by an in utero exposure phase, with refined arachidonic acid-rich oil (RAO) derived from Mortierella alpina XM027 in rats

A subchronic toxicity study, preceded by an in utero exposure phase, with refined arachidonic acid-rich oil (RAO) derived from Mortierella alpina XM027 in rats

Production and Enhancement of Omega-3 Fatty Acid fromMortierella alpinaCFR-GV15: Its Food and Therapeutic Application

Optimization of media components for enhanced arachidonic acid production by Mortierella alpina under submerged cultivation

Contact Info

Product

Resources

About