Safety Evaluation of Arsenicum album 6C in Cell Lines and BALB/c Mice Through Toxicity, Proliferation and Apoptosis Studies

Roy, Anirban; Sarkar, Avipsha; Parveen, Suraia; Ghosh, Paritosh; Nayak, Debadatta; Das, Satadal

doi:10.1007/s12595-023-00476-3

Proc Zool Soc

2023

DOI: 10.1007/s12595-023-00476-3

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Safety Evaluation of Arsenicum album 6C in Cell Lines and BALB/c Mice Through Toxicity, Proliferation and Apoptosis Studies

Anirban Roy¹,

Avipsha Sarkar²,

Suraia Parveen³

et al.

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2023

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Ultradiluted Eupatorium perfoliatum Alleviates DENV-induced Fibrosis By Regulation of TGFβ1, MMP-9 and Interferons

Sarkar,

Roy,

Maity

et al. 2023

Future Virol.

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Aim: This study aimed to alleviate dengue virus (DENV)-mediated fibrosis in a chick embryo model using ultradiluted Eupatorium perfoliatum (UEP). Materials & methods: Chorioallantoic membrane and liver of infected embryonated chicken eggs were checked for DENV pathogenesis. Cytotoxicity of UEP was assessed by MTT and scratch assay. Cytopathic assay after DENV infection was performed in vitro to check the effect of UEP and its constituents. Histopathology analysis revealed DENV-mediated fibrosis and the effect of UEP. qRT-PCR and gelatin zymography showed levels of fibrosis-causing genes. Results: UEP showed low cytotoxicity and maximum potential in mitigating DENV-mediated fibrosis in vivo via regulation of fibrosis biomarkers TGF-β, MMP-1, MMP-9, TIMP-1 and IFNs. Conclusion: UEP may be considered to alleviate DENV-mediated pathogenesis.

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Ultradiluted Eupatorium perfoliatum Alleviates DENV-induced Fibrosis By Regulation of TGFβ1, MMP-9 and Interferons

Sarkar,

Roy,

Maity

et al. 2023

Future Virol.

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show abstract

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Safety Evaluation of Arsenicum album 6C in Cell Lines and BALB/c Mice Through Toxicity, Proliferation and Apoptosis Studies

Cited by 1 publication

References 25 publications

Ultradiluted Eupatorium perfoliatum Alleviates DENV-induced Fibrosis By Regulation of TGFβ1, MMP-9 and Interferons

Ultradiluted Eupatorium perfoliatum Alleviates DENV-induced Fibrosis By Regulation of TGFβ1, MMP-9 and Interferons

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