Safety of Ethyl Acetate Fraction of Cocos Nucifera Root Extract.

Abstract

1083Cocos nucifera is a tropical coastal plant. The ethyl acetate fraction of its aqueous root extract stimulates erythropoiesis. This work aimed to evaluate the biological tolerance of this fraction. The extract fraction was administered to Wistar rats at a single dose of 2000 mg / kg body weight for Acute Oral Toxicity (OAT) or in daily doses of 200 mg / kg body weight for 28 days for Sub-Chronic Toxicity. (SCT), as recommended by the OECD. On day 0, then on day 14 for OAT and day 28 for SCT, the rats were weighed and their blood was collected for tests. The liver balance was assessed by measuring transaminases AST and ALT, kidney balance by uremia and creatinine level and immune status by blood leukocyte count. These analyzes were supplemented by the histology of the liver, kidneys and spleen, an immune organ. The weight of rats, the balance of liver, kidney and immunity as well as these organs histology were not affected by either acute oral or sub-chronic toxicity tests, suggesting a lack of toxicity in the extract fraction. The ethyl acetate fraction of the root water extract of Cocos nucifera did not reveal any acute or sub-chronic oral toxicity. This effect could be related to its richness in flavonoids that are antioxidants. The study of biological tolerance deserves to be continued by the chronic toxicity test and clinical trials.

The Ethyl Acetate Fraction of Psorospermum Febrifugum Spachroot Barks Aqueous Extract Is a Good Stimulator of Hematopoiesis

“…This result was better than that of the crude aqueous extract of Psorospermumfebrifugum, which minimum dose corrected the hemoglobin level only at day 15 (Agbogba et al, 2019a). On the other hand, it confirmed that observed with the ethyl acetate fraction of Cocos nucifera root aqueous extract (Sènou et al, 2017c). At day 15, the hemoglobin level was significantly greater than its D0 value only in the group receiving the 60 mg dose of extract / Kg (high dose) suggesting a dosedependent effect.…”

“…Mean cell volume(MCV) increased in all anemic groups with a peak on day 7 in the anemic and treated groups indicating early release into the bloodstream of macrocytes, immature red blood cells to compensate for anemia [31]. The globular volume then decreased progressively in the treated groups and at J15 no longer differed significantly from D0, indicating a well-differentiated red blood cells release at the end of the experimental period.…”

Ethyl acetate fraction of Psorospermum febrifugum Spach aqueous extract did not exhibit acute or sub-chronic toxicity. Experimental study on Wistar rats

“…For this purpose, we fractionated the extract in order to test some of its fractions. One of the fractions that we wanted to test was that of ethyl acetate because this fraction of the aqueous extract of Cocos nucifera showed a good hematopoietic activity (Sènou et al, 2017a). But first, it should first ensure the safety of this fraction of the extract before testing its efficacy.…”

“…Thus, 80% of people in poor countries use traditional medicine. The main reason was the high price of pharmaceutical drugs (Schmincke, 2003;Yakubu et al, 2009;Pingali et al, 2015). Therefore, many plants are used against anemia, some of which are very effective.…”

“…Therefore, many plants are used against anemia, some of which are very effective. That were the cases of Maguifera indica leaves and stem (Ogbe et al, 2010), Schrebera swietenioides root bark (Pingali et al, 2015), Moringa oleifera leaves (Nwaehujor et al, 2015) and Cocos nucifera root bark (Tchogou et al, 2016).…”