Safflower polysaccharide induces NSCLC cell apoptosis by inhibition of the Akt pathway

Li, Jianying; Yu, Jun; Du, Xusheng; Zhang, Huimin; Wang, Bo; Guo, Hua; Bai, Jie; Wang, Juanhong; Liu, An; Wang, Yili

doi:10.3892/or.2016.4784

Cited by 20 publications

(11 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Suppression of AKT activity by specific synthetic inhibitors of PI3K such as wortmannin and LY294002 has been revealed to exert antitumor activity against several human solid tumor models ( 42 ). Notably, several natural products, such as epigallocatechin gallate (EGCG) and safflower polysaccharide (SPS) inhibited AKT and induced apoptosis in NSCLC cells ( 43 , 44 ). EGCG significantly reduced the level of phosphorylated-AKT, but the same treatment did not affect the levels of total AKT expression ( 43 ).…”

Section: Discussionmentioning

confidence: 99%

Inactivation of AKT/NF‑κB signaling by eurycomalactone decreases human NSCLC cell viability and improves the chemosensitivity to cisplatin

et al. 2020

View full text Add to dashboard Cite

The high activation of protein kinase B (AKT)/nuclear factor-κB (NF-κB) signaling has often been associated with the induction of non-small cell lung cancer (NSCLC) cell survival and resistance to cisplatin, which is one of the most widely used chemotherapeutic drugs in the treatment of NSCLC. The inhibition of AKT/NF-κB can potentially be used as a molecular target for cancer therapy. Eurycomalactone (ECL), a quassinoid from Eurycoma longifolia Jack, has previously been revealed to exhibit strong cytotoxic activity against the human NSCLC A549 cell line, and can inhibit NF-κB activity in TNF-α-activated 293 cells stably transfected with an NF-κB luciferase reporter. The present study was the first to investigate whether ECL inhibits the activation of AKT/NF-κB signaling, induces apoptosis and enhances chemosensitivity to cisplatin in human NSCLC cells. The anticancer activity of ECL was evaluated in two NSCLC cell lines, A549 and Calu-1. ECL decreased the viability and colony formation ability of both cell lines by inducing cell cycle arrest and apoptosis through the activation of pro-apoptotic caspase-3 and poly (ADP-ribose) polymerase, as well as the reduction of anti-apoptotic proteins Bcl-xL and survivin. In addition, ECL treatment suppressed the levels of AKT (phospho Ser473) and NF-κB (phospho Ser536). Notably, ECL significantly enhanced cisplatin sensitivity in both assessed NSCLC cell lines. The combination treatment of cisplatin and ECL promoted cell apoptosis more effectively than cisplatin alone, as revealed by the increased cleaved caspase-3, but decreased Bcl-xL and survivin levels. Exposure to cisplatin alone induced the levels of phosphorylated-AKT and phosphorylated-NF-κB, whereas co-treatment with ECL inhibited the cisplatin-induced phosphorylation of AKT and NF-κB, leading to an increased sensitization effect on cisplatin-induced apoptosis. In conclusion, ECL exhibited an anticancer effect and sensitized NSCLC cells to cisplatin through the inactivation of AKT/NF-κB signaling. This finding provides a rationale for the combined use of chemotherapy drugs with ECL to improve their efficacy in NSCLC treatment.

show abstract

Section: Discussionmentioning

confidence: 99%

Inactivation of AKT/NF‑κB signaling by eurycomalactone decreases human NSCLC cell viability and improves the chemosensitivity to cisplatin

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Hydroxysafflor yellow A‐mediated sonodynamic therapy induced an autophagic response via the PI3K/Akt/mTOR signaling pathway and inhibited inflammation by reactive oxygen species in THP‐1 macrophages (Jiang et al, ). Safflower polysaccharide inhibited the proliferation and metastasis of MCF‐7 breast cancer cells and induced apoptosis of A549 and YTMLC‐90 non‐small lung cancer cells (Li, Yu, et al, ; Luo et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Safflower polysaccharide inhibited the proliferation and metastasis of MCF-7 breast cancer cells and induced apoptosis of A549 and YTMLC-90 non-small lung cancer cells (Li, Yu, et al, 2016;Luo et al, 2015).…”

Section: Carthami Flosmentioning

confidence: 96%

Herbal medicine for hand–foot syndrome induced by fluoropyrimidines: A systematic review and meta‐analysis

Deng

Sun

2018

Phytotherapy Research

View full text Add to dashboard Cite

The aims of this study were to evaluate the efficacy of herbal medicine for the prevention and management of hand-foot syndrome (HFS) induced by fluoropyrimidines and to identify herbs associated with HFS alleviation for further research. The PubMed, Cochrane, Springer, China National Knowledge Infrastructure, and Wanfang databases were searched up to May 2017 for randomized controlled trials (RCTs) that evaluated herbal medicine for relieving HFS in patients undergoing fluoropyrimidine-based chemotherapy. Study evaluation and synthesis methods were in accordance with the Cochrane Handbook, and data were analyzed using RevMan 5.3. In total, 35 RCTs (2,668 participants) were included. Meta-analysis showed that the addition of herbal medicine significantly reduced the incidences of all-grade and high-grade HFS. The total effective rate and complete remission rate of HFS patients increased significantly with herbal medicine arm. Further sensitivity analysis identified Paeoniae Radix Alba, Carthami Flos, Cinnamomi Ramulus, and Glycyrrhizae Radix et Rhizoma as being consistently associated with significant reductions in HFS incidence without important heterogeneity. However, the lack of blinding in most studies may have led to overestimation of these effects. More high-quality RCTs and experimental research are needed to confirm and investigate the efficacy of the herbs identified in this study.

show abstract

“…PI staining method was used to evaluate the cell cycle (Li et al., 2016 ). The cell culture and drug dosing were the same with cell apoptosis evaluation.…”

Section: Methodsmentioning

confidence: 99%

Hypoxic targeting and activating TH-302 loaded transcatheter arterial embolization microsphere

Chen

et al. 2020

Drug Delivery

View full text Add to dashboard Cite

The tumor-derived and transcatheter arterial chemoembolization (TACE) induced hypoxia microenvironment is closely related to the poor prognosis of hepatocellular carcinoma (HCC). In this study, hypoxia-activated prodrug TH-302 loaded poly(lactic-co-glycolic acid) (PLGA)-based TACE microspheres were prepared to treat HCC through localized and sustained drug delivery. TH-302 microspheres with three different sizes were fabricated by an oil-in-water emulsion solvent evaporation method and characterized by scanning electron microscopy (SEM), infrared spectra (IR), X-ray diffractometer (XRD), and drug release profiles. The in vitro antitumor potential was firstly evaluated in an HepG2 cell model under normoxic and hypoxic conditions. Then, a VX-2 tumor-bearing rabbit model was established and performed TACE to investigate the in vivo drug tissue distribution and antitumor efficiency of TH-302 microspheres. Blood routine examination and histopathological examinations were also conducted to evaluate the safety of TH-302 microspheres. TH-302 microspheres with particle size 75-100 lm, 100-200 lm, and 200-300 lm were prepared and characterized by sphere morphology and sustained drug release up to 360 h. Compared with TH-302, the microspheres exhibited higher cytotoxicity, cell apoptosis, and cell cycle S phase retardation in HepG2 cells under hypoxic conditions. The microspheres also displayed continuous drug release in the liver tissue and better anti-tumor efficiency compared with TH-302 injection and lipiodol. Meanwhile, no serious toxicity appeared in the duration of treatment. Therefore, TH-302 microspheres showed to be feasible and effective for TACE and hold promise in the clinical for HCC chemoembolization therapy.

show abstract

Safflower polysaccharide induces NSCLC cell apoptosis by inhibition of the Akt pathway

Cited by 20 publications

References 31 publications

Inactivation of AKT/NF‑κB signaling by eurycomalactone decreases human NSCLC cell viability and improves the chemosensitivity to cisplatin

Inactivation of AKT/NF‑κB signaling by eurycomalactone decreases human NSCLC cell viability and improves the chemosensitivity to cisplatin

Herbal medicine for hand–foot syndrome induced by fluoropyrimidines: A systematic review and meta‐analysis

Hypoxic targeting and activating TH-302 loaded transcatheter arterial embolization microsphere

Contact Info

Product

Resources

About