Safflower Yellow regulates microglial polarization and inhibits inflammatory response in LPS-stimulated Bv2 cells

Yang, Xiaotao; Li, Yanhua; Zhang, Hui; Zhao, Yongfei; Ding, Zhi-Bin; Yu, Jie‐Zhong; Liu, Chunyun; Liu, Jianchun; Jiang, Weijia; Feng, Qi; Xiao, Bailu; Ma, Chi

doi:10.1177/0394632015617065

Cited by 48 publications

(35 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Microglia mediates multiple facets of neuroinflammation [3], which is a significant pathological process involved in all types of CNS disorders including epilepsy [2]. Switching microglial polarization from the pro-inflammatory Fig.…”

Section: Discussionmentioning

confidence: 99%

Dynorphin activation of kappa opioid receptor promotes microglial polarization toward M2 phenotype via TLR4/NF-κB pathway

Liu

Dai

et al. 2020

Cell Biosci

View full text Add to dashboard Cite

Background: Microglia-mediated neuroinflammation is associated with epilepsy. Switching microglial polarization from the pro-inflammatory M1 phenotype to the anti-inflammatory M2 phenotype represents a novel therapeutic strategy for mitigating epileptogenesis. We previously found that dynorphins protected against epilepsy via activation of kappa opioid receptor (KOR). Here, this study aims to investigate the role and the mechanism of dynorphin in regulating microglial polarization. Methods: A pilocarpine-induced rat model of epilepsy was established and lipopolysaccharide (LPS)-activated BV-2 microglial cells were used as an inflammatory model to explore the mechanism of dynorphin regulating microglial polarization. Results: Overexpression of the dynorphin precursor protein prodynorphin (PDYN) alleviated the pilocarpine-induced neuronal apoptosis, promoted microglial polarization to the M2 phenotype, and inhibited pilocarpine-induced Tolllike receptor 4 (TLR4)/nuclear factor-kappa B (NF-κB) pathway in the hippocampi of epileptic rats. Dynorphin activation of KOR promoted microglial M2 polarization via inhibiting TLR4/NF-κB pathway in LPS-stimulated BV-2 microglial cells. Moreover, dynorphin/KOR regulated microglial M2 polarization inhibited apoptosis of the primary mouse hippocampal neurons. Conclusion: In conclusion, dynorphin activation of KOR promotes microglia polarization toward M2 phenotype via inhibiting TLR4/NF-κB pathway.

show abstract

Section: Discussionmentioning

confidence: 99%

Dynorphin activation of kappa opioid receptor promotes microglial polarization toward M2 phenotype via TLR4/NF-κB pathway

Liu

Dai

et al. 2020

Cell Biosci

View full text Add to dashboard Cite

show abstract

“…Overactivation of the NF-κB signalling pathway causes the excessive production of pro-inflammatory cytokines and triggers an array of microglia responses leading to the release of inflammatory mediators [16,30]. A recent study has shown that LPS can shift microglia from M0 to M1 phenotype by stimulating the activation of TLR4/p-38/p-JNK/NF-κB signalling pathway [36]. LPS binds to TLR4 on the surface of microglia cells, resulting in the overexpression of proinflammato- .…”

Section: Discussionmentioning

confidence: 99%

Astragaloside IV protects neurons from microglia-mediated cell damage through promoting microglia polarization

Yu¹,

Guo²,

Lǐ³

et al. 2019

View full text Add to dashboard Cite

Astragaloside IV (AST-IV) is a major active ingredient of astragalus, with a neuroprotective effect. The current study is aimed to investigate the impact of AST-IV on the M1/M2 microglial activation in response to lipopolysaccharide (LPS) stimulation, how AST-IV attenuated microglia-mediated neuronal damage, and the molecular mechanisms underlying AST-IV's protection of neurons against microglia-mediated neuronal damage. Our results showed that AST-IV partially protected microglia from death evoked by LPS and downregulated the release of pro-inflammatory (M1) mediators including interleukin (IL)-1β, IL-6, tumour necrosis factor α (TNF-α) and nitric oxide, as well as the expression of Toll-like receptors 4 (TLR4), MyD88, and nuclear factor κB (NF-κB) of these cells. In contrast, AST-IV elevated the production of anti-inflammatory cytokine IL-10 and expression of arginase 1, an M2 marker of microglia, whose conditioned medium promoted PC12 neurons survival. These results indicate that AST-IV exerts an anti-inflammatory effect on microglia, possibly through inhibiting TLR4/NF-κB signalling pathways, and protects neurons from microglia-mediated cell death through conversion of microglia from inflammatory M1 to an anti-inflammatory M2 phenotype.

show abstract

“… 29 We recently reported that tetramethoxyluteolin inhibits human microglia proliferation 30 and so do flavonoids from safflower yellow. 31 Flavonoids are generally considered safe 32 , 33 and now being increasingly discussed for the treatment of neurodegenerative disorders. 34 …”

Section: Discussionmentioning

confidence: 99%

Tolerability and benefit of a tetramethoxyluteolin-containing skin lotion

Theoharides

Stewart

Tsilioni

2017

Int J Immunopathol Pharmacol

View full text Add to dashboard Cite

As many as 40% of people have sensitive skin and at least half of them suffer from pruritus associated with allergies, atopic dermatitis (AD), chronic urticaria (CU), cutaneous mastocytosis (CM), and psoriasis. Unfortunately, the available topical formulations contain antihistamines that are often not as effective as those containing corticosteroids. Certain natural flavonoids have anti-inflammatory actions. We recently reported that the natural flavonoid tetramethoxyluteolin has potent antiallergic and anti-inflammatory actions in vitro and in vivo. This flavonoid was formulated in a skin lotion along with olive fruit extract and was first tested for tolerability in 25 patients with mastocytosis or mast cell activation syndrome and very sensitive skin who reported back through a questionnaire. The skin lotion was then used by eight patients, four with AD and four with psoriasis, who had not received any topical treatment for at least 2 months, twice daily for 2 weeks. The use of this tetramethoxyluteolin formulation resulted in significant improvement of the skin lesions and could be useful adjuvant treatment for allergic and inflammatory skin conditions.

show abstract

Safflower Yellow regulates microglial polarization and inhibits inflammatory response in LPS-stimulated Bv2 cells

Cited by 48 publications

References 33 publications

Dynorphin activation of kappa opioid receptor promotes microglial polarization toward M2 phenotype via TLR4/NF-κB pathway

Dynorphin activation of kappa opioid receptor promotes microglial polarization toward M2 phenotype via TLR4/NF-κB pathway

Astragaloside IV protects neurons from microglia-mediated cell damage through promoting microglia polarization

Tolerability and benefit of a tetramethoxyluteolin-containing skin lotion

Contact Info

Product

Resources

About