Salicylaldehyde synthase activity from Venturia inaequalis elicitor-treated cell culture of apple

Sarkate, Amol; Kumar, Pranav; Sharma, Ashwani; Sircar, Debabrata

doi:10.1016/j.jplph.2017.12.002

Cited by 7 publications

(3 citation statements)

References 38 publications

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“…The following in a phenylalanine to salicylaldehyde metabolism might first require conversion of transcinnamic acid to o-coumaric acid. The terminal conversion of o-coumaric acid to salicylaldehyde is known in tobacco plants 106 , as well as in a species of fungus 107 , although bacterial catalysis of this terminal reaction is not characterized. While this might represent a parsimonious explanation for the biosynthesis of salicylaldehyde, more work is warranted to establish the distribution of microbial participation in these pathways, especially with respect to Pelomonas.…”

Section: Discussionmentioning

confidence: 99%

Gut microbiota metabolically mediate intestinal helminth infection in Zebrafish

Hammer,

Gaulke,

García - Jaramillo

et al. 2024

Preprint

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Intestinal helminth parasite (IHP) infection induces alterations in the composition of microbial communities across vertebrates, although how gut microbiota may facilitate or hinder parasite infection remains poorly defined. In this work we utilized a zebrafish model to investigate the relationship between gut microbiota, gut metabolites, and IHP infection. We found that extreme disparity in zebrafish parasite infection burden is linked to the composition of the gut microbiome, and that changes in the gut microbiome are associated with variation in a class of endogenously-produced signaling compounds, N-acylethanolamines, that are known to be involved in parasite infection. Using a statistical mediation analysis, we uncovered a set of gut microbes whose relative abundance explains the association between gut metabolites and infection outcomes. Experimental investigation of one of the compounds in this analysis reveals salicylaldehyde, which is putatively produced by the gut microbe Pelomonas, as a potent anthelmintic with activity against Pseudocapillaria tomentosa egg hatching, both in vitro and in vivo. Collectively, our findings underscore the importance of the gut microbiome as a mediating agent in parasitic infection and highlights specific gut metabolites as tools for the advancement of novel therapeutic interventions against IHP infection.

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Section: Discussionmentioning

confidence: 99%

Gut microbiota metabolically mediate intestinal helminth infection in Zebrafish

Hammer,

Gaulke,

García - Jaramillo

et al. 2024

Preprint

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“…In this study we have used apple cell culture as model system because cell cultures offer an uniform plant material for studying metabolic changes and typically plant cell cultures exhibit relatively less metabolic complexity due to lack of pigments and lignified tissues. Earlier plant cell cultures have been successfully used for analyzing plant-microbe interactions in apple and other plant systems 29,45,46 . The metabolomics data were then correlated with the expression patterns of genes linked to selected secondary metabolite biosynthesis to obtain a comprehensive insight of the changes occurring after VIE treatment.…”

Section: Discussionmentioning

confidence: 99%

“…Earlier, it was reported that the treatment of V . inaequalis elicitor induces synthesis of salicylic acid in the cell suspensions of apple cultivar ‘Florina’ 29 . The elicitor-induced formation of a novel phytoalexin malusfuran was reported from the cell suspensions of scab resistant cultivar ‘Liberty’ 28 .…”

Section: Introductionmentioning

confidence: 99%

Comparative metabolomics of scab-resistant and susceptible apple cell cultures in response to scab fungus elicitor treatment

Sarkate

Teotia

Gaid

et al. 2018

Sci Rep

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Apple scab disease caused by the fungus Venturia inaequalis is a devastating disease that seriously affects quality and yield of apples. In order to understand the mechanisms involved in scab resistance, we performed gas chromatography-mass spectrometry based metabolomics analysis of the cell culture of scab resistant cultivar ‘Florina’ and scab susceptible cultivar ‘Vista Bella’ both prior -to and -following treatment with V. inaequalis elicitor (VIE). A total 21 metabolites were identified to be altered significantly in ‘Florina’ cell cultures upon VIE-treatment. Among 21 metabolites, formation of three new specialized metabolites aucuparin, noraucuparin and eriobofuran were observed only in resistant cultivar ‘Florina’ after the elicitor treatment. The score plots of principal component analysis (PCA) exhibited clear discrimination between untreated and VIE-treated samples. The alteration in metabolite levels correlated well with the changes in the transcript levels of selected secondary metabolite biosynthesis genes. Aucuparin, noraucuparin and eriobofuran isolated from the ‘Florina’ cultures showed significant inhibitory effect on the conidial germination of V. inaequalis. The results expand our understanding of the metabolic basis of scab-resistance in apple and therefore are of interest in apple breeding programs to fortify scab resistance potential of commercially grown apple cultivars.

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