Salidroside can target both P4HB-mediated inflammation and melanogenesis of the skin

Ding, Xiujuan; Zhang, Zhiyuan; Jin, Jing; Wang, Yan; Yang, Kai; Yang, Yankun; Wang, Hongqi; Dai, Xintong; Yao, Cheng Wen; Sun, Tao; Zhu, Caibin; Liu, Huijuan

doi:10.7150/thno.47413

Cited by 41 publications

(21 citation statements)

References 22 publications

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“…For intracellular experiments, the human melanocyte line that depends on the activation of TRY pathway is a key element in efficiently evaluating the anti-melanin synthesis effect of WFP. The A375 cell line, a metastatic cell line of the human amelanotic melanoma, has been widely used as a reliable model for screening anti-melanin agents [ 41 – 43 ], which is dependent on TRY pathway activation [ 44 , 45 ]. Firstly, thecytotoxic effect of WFPin A375 cells should be evaluated, and eliminate the influence caused by the decease of melanin synthesis due to cell numbers decline.…”

Section: Discussionmentioning

confidence: 99%

Anti-melanogenesis effect from Wampee fruit pectin via α-MSH/TRY pathway in A375 cells

Liao

Zhang

et al. 2022

BMC Complement Med Ther

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Background Polysaccharides from wampee have been reported to process various biological activities, while the relationship between structure and bioactivities has been barely addressed. Pectin, an abundant water-soluble polysaccharide in wampee, showed significant antioxidant activity, which was associated with the anti-melanogenic activity. Therefore, this study investigated the physicochemical characteristics and the anti-melanogenesis effect of pectin extracted from wampee fruit in A375 cells. Methods The physicochemical characterization of pectin from wampee fruit was investigated by gel chromatography (GCP), FT-IR spectroscopy, and NMR spectroscopy methods. The anti-melanogenesis effects and mechanism were evaluated by mushroom tyrosine enzyme and human melanin cell model in vitro. Results The results showed that a molecular weight of 5.271 × 105 Da wampee fruit pectin (WFP) were mainly composed of mannose (Man), ribose (Rib), rhamnose (Rha), glucuronic acid (Glc A), glucose (Glc), galacturonic acid (Gal A), galactose (Gal), and arabinose (Ara), which linked with →4)-β-D-Galp-(1 → units. The current study revealed that WFP could significantly suppress mushroom TRY activity in vitro. Furtherly, WFP significantly reduced intracellular and extracellular melanin formation in A375 melanoma cells depending on the presence of alpha-melanocyte stimulating hormone (α-MSH). TRY activity was only inhibited in α-MSH treated A375 cells. Western blot analysis demonstrated that WFP reverse α-MSH induced melanogenesis in A375 melanoma cells, including in down-regulated TRY, TYRP-1, TYRP-2, MITF and CREB expressions. Conclusion These results indicated that WFP could inhibit α-MSH induced melanogenesis in A375 melanoma cells via α-MSH/TRY pathway. In conclusion, these data provided a new perspective to annotate WFP anti-melanogenesis activity mechanism.

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Section: Discussionmentioning

confidence: 99%

Anti-melanogenesis effect from Wampee fruit pectin via α-MSH/TRY pathway in A375 cells

Liao

Zhang

et al. 2022

BMC Complement Med Ther

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“…Elevated expression of P4HB has been reported in several solid tumors, such as ovarian cancer ( Bonome et al, 2008 ), bladder cancer ( Lyu et al, 2020 ; Wang et al, 2020 ), and prostate cancer ( Welsh et al, 2001 ; Singh et al, 2002 ). Additionally, Ding et al (2020) reported that targeting P4HB can reduce inflammation and melanogenesis of the skin. Furthermore, previous studies found that PDIA1 contributes to oxidative maturation of proinsulin in the endoplasmic reticulum to support insulin production and ß-cell health in diet-induced obesity ( Jang et al, 2019 ), indicating that P4HB could indirectly influence insulin production and ß-cell health.…”

Section: Discussionmentioning

confidence: 99%

Identification and validation of P4HB as a novel autophagy-related biomarker in diabetic nephropathy

Bai

Yang

et al. 2022

Front. Genet.

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Diabetic nephropathy (DN), a frequent microvascular complication of diabetes, has been recognized as a primary cause of chronic kidney disease (CKD) and end-stage renal disease (ESRD). Previous studies found that autophagy of renal tubular epithelial cells plays an important role in DN pathogenesis. Our research aimed to investigate the differentially expressed autophagy-related genes (DEARGs) between DN and healthy renal tubule samples and identify a novel autophagy-related biomarker associated with tubulointerstitial injury in DN. In this study, gene expression profiles of renal tubules from 10 DN patients and 24 healthy controls in the GSE30122 dataset were analyzed, and 43 DEARGs were identified by bioinformatics analysis. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis and correlation analysis were performed on DEARGs, and the hub gene prolyl 4-hydroxylase subunit beta (P4HB) was screened by protein–protein interaction and verified by utilizing other datasets and stimulating HK-2 cells under high glucose concentration. We found that the expression of P4HB in renal tubules was correlated with renal function. In summary, our research provided novel insights for comprehension of DN molecular mechanisms and identified P4HB as a novel autophagy-related biomarker of DN.

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“…Melanin was detected according to the method of Ding et al (2020). B16‐F10 cells were seeded at a density of 50% in six‐well plates.…”

Section: Methodsmentioning

confidence: 99%

A non‐retinol retinoic acid receptor‐γ (RAR‐γ/NR1B3) selective agonist, tectorigenin, can effectively inhibit the ultraviolet A‐induced skin damage

Dai

Jin²,

Jia

et al. 2022

British J Pharmacology

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Background and Purpose Long‐term ultraviolet (UV) exposure can cause inflammation, pigmentation and photoaging. All‐trans retinoic acid (ATRA/tretinoin) is a commonly used retinoic acid receptor (RAR) agonist in the clinical treatment of UV‐induced skin problems. However, the use of such drugs is often accompanied by systemic adverse reactions caused by nonspecific activation of RARs. Therefore, this study was designed to screen for a novel RAR‐γ‐selective agonist with high safety. Experimental Approach Molecular docking, dynamic simulation and Biacore were used to screen and identify novel RAR‐γ‐selective agonists. RT‐PCR, ELISA, western blotting, immunofluorescence staining, flow cytometry and proteomic analysis were used to detect the effects of these novel RAR‐γ selective agonists on UVA‐induced inflammation and photoaging cell models. UVA‐induced mouse models were used to evaluate the effects of tectorigenin on skin repair, ageing and inflammation. Key Results Tectorigenin is a novel RAR‐γ‐selective agonist, which inhibits UV‐induced oxidative damage, inflammatory factor release and matrix metalloproteinase (MMP) production. Tectorigenin can also reverse the UVA‐induced loss of collagen. The results of the signalling pathway research showed that tectorigenin mainly affects the MAPK/JNK/AP‐1 pathway. In animal experiments, tectorigenin showed better anti‐inflammatory and anti‐photoaging effects, and caused less skin irritation than ATRA. Nano‐particle loaded tectorigenin significantly improved the utilization of tectorigenin. Conclusions and Implications Tectorignen is a non‐retinol RAR‐γ‐selective agonist that can inhibit UV‐induced skin damage and could be developed as a safe pharmaceutical component for the prevention of photoaging and skin inflammation.

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Salidroside can target both P4HB-mediated inflammation and melanogenesis of the skin

Cited by 41 publications

References 22 publications

Anti-melanogenesis effect from Wampee fruit pectin via α-MSH/TRY pathway in A375 cells

Anti-melanogenesis effect from Wampee fruit pectin via α-MSH/TRY pathway in A375 cells

Identification and validation of P4HB as a novel autophagy-related biomarker in diabetic nephropathy

A non‐retinol retinoic acid receptor‐γ (RAR‐γ/NR1B3) selective agonist, tectorigenin, can effectively inhibit the ultraviolet A‐induced skin damage

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