Salinity alters snakeskin and mesh transcript abundance and permeability in midgut and Malpighian tubules of larval mosquito, Aedes aegypti

Jonusaite, Sima; Donini, Andrew; Kelly, Scott P.

doi:10.1016/j.cbpa.2016.12.015

Cited by 24 publications

(34 citation statements)

References 29 publications

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“…In a second study, a salinity-induced increase in the protein abundance of the integral SJ protein kunekune (Kune) was observed in the posterior midgut as well as anal papillae of A. aegypti larva while other SJ proteins were unaltered (Jonusaite et al, 2016b). Taken together, these observations suggest that select SJ proteins contribute to osmoregulatory homeostasis in larval A. aegypti (Jonusaite et al, 2016b(Jonusaite et al, , 2017.…”

Section: Introductionmentioning

confidence: 80%

“…3 H]polyethylene glycol (molecular mass 400 Da; 'PEG-400'; American Radiolabeled Chemicals, St Louis, MO, USA) across the midgut epithelium of larval A. aegypti was determined as previously described (Jonusaite et al, 2017). […”

Section: Flux Of the Paracellular Permeability Marker [mentioning

confidence: 99%

“…Following this, Gli protein abundance was examined as well as paracellular permeability in the midgut using the midgut permeability assay (see Jonusaite et al, 2017). Larvae treated with gli dsRNA showed a significant reduction in ∼150 kDa Gli protein abundance in the anterior midgut at day 2 post-gli dsRNA treatment compared with the group treated with βLac dsRNA (Fig.…”

Section: Effect Of Gli Dsrna Knockdown On Midgut Permeabilitymentioning

confidence: 99%

“…This suggests that alterations in the molecular physiology of aquatic arthropod SJs in osmoregulatory epithelia should be expected in response to changes in the ionic strength of their surroundings, and two recent studies support this hypothesis. The first (Jonusaite et al, 2017) reported that the flux rate of the paracellular permeability marker PEG-400 was greater across the midgut epithelium of BW-reared larval A. aegypti when compared with organisms reared in FW, while the Malpighian tubules had reduced PEG-400 permeability in BW versus FW larvae. The changes in PEG-400 flux across the midgut and Malpighian tubules occurred in association with increased transcript abundance of the sSJ proteins Ssk and mesh in these epithelia of BW-reared larvae when compared with FW animals (Jonusaite et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…The first (Jonusaite et al, 2017) reported that the flux rate of the paracellular permeability marker PEG-400 was greater across the midgut epithelium of BW-reared larval A. aegypti when compared with organisms reared in FW, while the Malpighian tubules had reduced PEG-400 permeability in BW versus FW larvae. The changes in PEG-400 flux across the midgut and Malpighian tubules occurred in association with increased transcript abundance of the sSJ proteins Ssk and mesh in these epithelia of BW-reared larvae when compared with FW animals (Jonusaite et al, 2017). In a second study, a salinity-induced increase in the protein abundance of the integral SJ protein kunekune (Kune) was observed in the posterior midgut as well as anal papillae of A. aegypti larva while other SJ proteins were unaltered (Jonusaite et al, 2016b).…”

Section: Introductionmentioning

confidence: 99%

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Identification of the septate junction protein gliotactin in the mosquito, Aedes aegypti: evidence for a role in increased paracellular permeability in larvae

Jonusaite

Kelly

Donini

2017

Journal of Experimental Biology

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Septate junctions (SJs) regulate paracellular permeability across invertebrate epithelia. However, little is known about the function of SJ proteins in aquatic invertebrates. In this study, a role for the transmembrane SJ protein gliotactin (Gli) in the osmoregulatory strategies of larval mosquito (Aedes aegypti) was examined. Differences in gli transcript abundance were observed between the midgut, Malpighian tubules, hindgut and anal papillae of A. aegypti, which are epithelia that participate in larval mosquito osmoregulation. Western blotting of Gli revealed its presence in monomer, putative dimer and alternatively processed protein forms in different larval mosquito organs. Gli localized to the entire SJ domain between midgut epithelial cells and showed a discontinuous localization along the plasma membranes of epithelial cells of the rectum as well as the syncytial anal papillae epithelium. In the Malpighian tubules, Gli immunolocalization was confined to SJs between the stellate and principal cells. Rearing larvae in 30% seawater caused an increase in Gli protein abundance in the anterior midgut, Malpighian tubules and hindgut. Transcriptional knockdown of gli using dsRNA reduced Gli protein abundance in the midgut and increased the flux rate of the paracellular permeability marker, polyethylene glycol (molecular weight 400 Da; PEG-400). Data suggest that in larval A. aegypti, Gli participates in the maintenance of salt and water balance and that one role for Gli is to participate in the regulation of paracellular permeability across the midgut of A. aegypti in response to changes in environmental salinity.

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Section: Introductionmentioning

confidence: 80%

Section: Flux Of the Paracellular Permeability Marker [mentioning

confidence: 99%

Section: Effect Of Gli Dsrna Knockdown On Midgut Permeabilitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%