Salinity and drought stress on barley and wheat cultivars planted in Turkey

Abstract

The study was conducted to investigate the salinity and drought susceptibilities of barley and wheat varieties cultivated in Turkey by phenotypic assays and to evaluate the association of WRKY19 and WRKY52 transcription factors with salinity and drought stresses by gene expression assays. Salinity tests of treatment different concentration of (0, 0.5, 1 and 2%) NaCl were managed with 13 barley and 22 wheat varieties germinated for 10 days. According to findings obtained from phenotypic investigations, two relative tolerant and two sensitive cultivars were selected and used in gene expression analysis. Transcript abundance for drought stress and relative fold changes for salinity stress were analyzed via expression assays of WRKY19 and WRKY52 genes. The minimum and maximum germination scores were changed between 0.090±0.090-3.818±0.400 (barley) and 0.454±0.312-3.0913±0.594 (wheat), while water loss rate (WLR) values ranged from 0.009±0.0091-0.2±0.0011 (barley) and 0.01±0.0005-0.3±0.1195 (wheat). In drought stress assessments, WRKY19 and WRKY52 transcripts abundances were relatively higher in relatively resistance cultivars in comparison to sensitive genotypes. Similarly, fold c h a n g e s i n g e n e expression were higher in resistant cultivars up to +26 changes. Drought and salinity stress factor analysis showed that there was no homogenous abiotic stress response profile for barley and wheat varieties in Turkey. According to gene expression analysis, WRKY19 and WRK52 genes could stimulate the d r o u g h t a n d s a l i n i t y responses. This study is important in terms of a n a l y z i n g t h e c e r e a l varieties planted in Turkey, a n d p r o v i d i n g a n a s s o c i a t i o n b e t w e e n WRKY genes and abiotic stress.

Physiological and Genetic Variation of Hordeum vulgare L. and Triticum aestivum L. Lines Planted in Turkey

“…In this study, we showed that there was great variation in EC capacity of barley and wheat lines. Similarly, in comparison to previous studies relatively drought resistant lines, which could be used in further studies, were detected (Suprunova et al, 2004;Yörük et al, 2018).…”

“…Barley and wheat have a great significance in economies of many countries including Turkey. Several investigations have been conducted on drought and salinity response of barley and wheat cultivars planted in Turkey (Gürel et al, 2016;Yörük et al, 2018;Tufan et al, 2020). However, limited number of barley and wheat lines were investigated in terms of physiological and genetic differences of lines planted/cultivated in Turkey.…”

Fusarium culmorum ile Enfekte Edilen Arpa Çeşitlerinde WRKY Transkripsiyon Faktörlerinin Analizi

“…WRKY, bZIP, MYB, bHLH, AP2/ERF, NAC and homeodomain TFs have been shown to participate in the regulation of stress responses [6,7,8,9,10]. WRKYs have vital roles in plant defense against abiotic and biotic stresses as well as involved in many developmental processes such as seed development, dormancy, leaf senescence, and trichome development and some signal transduction processes mediated by plant growth regulators [11,12,13]. WRKYs have conserved 60 amino acid regions comprising of the highly conserved WRKYGQK peptide sequence and a zinc finger like motif.…”

Buğday (Triticum aestivum L.) ve arpada (Hordeum vulgare L.) tuzluluk stres yanıtı ile ilişkili WRKY transkripsiyon faktörlerinin belirlenmesi

“…According to the findings, no germination was observed in 2.0 % NaCl treated experiment sets. Significantly decreased to 2.0 % NaCl treatment in wheat and barley cultivars was an expected result since NaCl causes strong salt stress for many plant species (Badridze et al, 2009;Almodares et al, 2014;Jain et al, 2016;Yörük et al, 2018). Thus, further transcript analysis was carried with 0 % and 1.0 % NaCl treated sets.…”