Salinity-mediated carbonic anhydrase induction in the gills of the euryhaline green crab, Carcinus maenas

Henry, Raymond P.; Gehnrich, Stephen; Weihrauch, Dirk; Towle, David W.

doi:10.1016/s1095-6433(03)00113-2

Cited by 133 publications

(111 citation statements)

References 39 publications

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“…Our results were similar to study by Wang et al, who revealed those two genes are sensitive and involved in all different stress responses and are more sensitive to salinity than other stresses may have relationship with the anti-stress mechanism induced by environment stress in shrimp (Wang et al, 2012). The carbonic anhydrase (CA) is a zinc metalloenzymes which involves in many biological processes, including pH adjustment, ion exchange, CO 2 transport, respiration, biosynthesis and so on (Henry, Gehnrich, Weihrauch, & Towle, 2003 (Morris, 2001). The vesicle transport protein can transport synthesized proteins from endoplasmic reticulun to the apical and basolateral membrane.…”

Section: Transportsupporting

confidence: 91%

Profiling of Up-Regulated Genes Response to Acute Hypo-Osmotic Stress in Hepatopancreas and Gill of the Pacific White Shrimps (Litopenaeus vannamei)

Liu¹,

Sun²,

Dong³

et al. 2016

IJB

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Suppression subtractive hybridization (SSH) was applied to screen responsively up-regulation genes in hepatopancreas and gill of Litopenaeus vannamei induced by acute hypo-osmotic stress. In the hepatopancreas, 196 clones were randomly selected and sequenced. 131 non-redundant transcripts, corresponding to 41 genes, were found with elevated expressions. They were functionally clustered into eight biological processes which were protein synthesis and processing, carbohydrate metabolism and energy production, transport, cell growth, apoptosis, cytoskeletal component, cell defense and homeostasis, signal transduction, accounting for 26.2%, 14.3%, 16.7%, 4.7%, 4.7%, 2.3%, 11.9% and 7.1%, respectively. When confirmed by real time qRT-PCR, the gene expression levels of MnSOD, glutathione and glutamine synthetase showed significant increases (2.64-folds, 3.44-folds and 2.16-folds, respectively) in hepatopancreas (P<0.05). Totally 81 expressed sequence tags (ESTs) were obtained by random sequencing from the gill SSH cDNA library, and 52 unigenes, including 15 contigs and 37 singlets, after CP3 online matching were gained by clustering and assembling. The sequence alignment results revealed that 30 of them had significant homology to previously identified Genbank database sequences, while 22 unigenes did not match any sequence in the datebase and presumably represented unidentified cDNAs. Functionally, the unigenes were classified into six clusters, including ion channels and transport proteins (1); protein synthesis, translation and transcription factor (2); stress resistance and antioxidant factors (3); energy metabolism (4); signal receptor and transduction (5); cell fibrin and cytoskeletal proteins (6) with proportion of 23.3%, 20.0%, 20.0%, 16.7%, 10.0% and 10.0% respectively. Real time qRT-PCR confirmation revealed significant elevation of Arginine kinase, Carbonic anhydrase and NaK-ATPase-α-subunit expressions (3.73-folds, 2.55-folds and 5.83-folds, respectively) in the gill after acute low-osmotic stress (P < 0.05). These results provided insight into critical physiology processes and pathways constituting the hypo-osmotic stress adaption program in hepatopancreas and gill of L. vannamei.

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Section: Transportsupporting

confidence: 91%

Profiling of Up-Regulated Genes Response to Acute Hypo-Osmotic Stress in Hepatopancreas and Gill of the Pacific White Shrimps (Litopenaeus vannamei)

Liu¹,

Sun²,

Dong³

et al. 2016

IJB

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“…Besides MT as a possible ligand for Zn binding, another explanation of stronger Zn elution in samples from March obtained at location H compared to location M and R could be some Zn associated enzyme, possibly carbonic anhydrase (CA). The activity of CA can be linked with osmoregulation in organisms living in an environment of changing salinity (Henry and Saintsing, 1983;Henry et al, 2003) as was the case for the estuarine location of the harbor investigated in the current study. In particular in March the salinity was very low due to the river input.…”

Section: Discussionmentioning

confidence: 67%

Characterization of the cytosolic distribution of priority pollutant metals and metalloids in the digestive gland cytosol of marine mussels: Seasonal and spatial variability

Strižak

Ivanković

Pröfrock

et al. 2014

Science of The Total Environment

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“…The gills of Crustacea form an interface between internal milieu and their environment and play a key osmorespiratory role. As a multifunctional organ they serve in: gas exchange (Burnett and Stickle, 2001), osmolyte transport, acid base and volume regulation (Gilles and Péqueux, 1985;Henry et al, 2002Henry et al, , 2003Weihrauch et al, 2002;Fehsenfeld et al, 2011), immune functions (Burnett and Burnett, 2015) and detoxification (Ahearn et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

The effects of hypoxia on active ionic transport processes in the gill epithelium of hyperregulating crab, Carcinus maneas

Lucu

Ziegler

2017

Comparative Biochemistry and Physiology Part A: Molecular &

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A B S T R A C TEffects of hypoxia on the osmorespiratory functions of the posterior gills of the shore crab Carcinus maenas acclimated to 12 ppt seawater (DSW) were studied. Short-circuit current (Isc) across the hemilamella (one epithelium layer supported by cuticle) was substantially reduced under exposure to 1.6, 2.0, or 2.5 mg O 2 /L hypoxic saline (both sides of epithelium) and fully recovered after reoxygenation. Isc was reduced equally in the epithelium exposed to 1.6 mg O 2 /L on both sides and when the apical side was oxygenated and the basolateral side solely exposed to hypoxia. Under 1.6 mg O 2 /L, at the level of maximum inhibition of Isc, conductance was decreased from 40.0 mS cm − 2 to 34.7 mS cm − 2 and fully recovered after reoxygenation. Isc inhibition under hypoxia and reduced 86 Rb + (K + ) fluxes across apically located K + channels were caused preferentially by reversible inhibition of basolaterally located and ouabain sensitive Na + ,K + -ATPase mediated electrogenic transport. Reversible inhibition of Isc is discussed as decline in active transport energy supply down regulating metabolic processes and saving energy during oxygen deprivation. In response to a 4 day exposure of Carcinus to 2.0 mg O 2 /L, hemolymph Na + and Cl − concentration decreased, i.e. hyperosmoregulation was weakened. Variations of the oxygen concentration level and exposure time to hypoxia lead to an increase of the surface of mitochondria per epithelium area and might in part compensate for the decrease in oxygen availability under hypoxic conditions.

show abstract

Salinity-mediated carbonic anhydrase induction in the gills of the euryhaline green crab, Carcinus maenas

Cited by 133 publications

References 39 publications

Profiling of Up-Regulated Genes Response to Acute Hypo-Osmotic Stress in Hepatopancreas and Gill of the Pacific White Shrimps (Litopenaeus vannamei)

Profiling of Up-Regulated Genes Response to Acute Hypo-Osmotic Stress in Hepatopancreas and Gill of the Pacific White Shrimps (Litopenaeus vannamei)

Characterization of the cytosolic distribution of priority pollutant metals and metalloids in the digestive gland cytosol of marine mussels: Seasonal and spatial variability

The effects of hypoxia on active ionic transport processes in the gill epithelium of hyperregulating crab, Carcinus maneas

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