Salinity tolerance in Senna macranthera (DC. ex Collad.) H. S. Irwin & Barneby seeds with sodium nitroprusside (SNP) promoted by cyanide

Abstract

Studies show the positive effect of the nitric oxide donor in the seed germination process. However, there are no precise reports about which compound present in sodium nitroprusside would cause these effects in seeds under salt stress. The aim of this study was to evaluate the effects of SNP on germination of Senna macranthera seeds under salt stress. The osmotic potentials of -0.3, -0.4, and -0.5 MPa of NaCl were used, as well as the concentration of 100 μM of sodium nitroprusside, inactive sodium nitroprusside, and ferrocyanide. The sodium nitroprusside in germination was applied before, during, and after salt stress. Germination rate, germination speed index, percentage of normal seedlings, and seedling development components were evaluated. The treatments that proved to be more promising in recovery of germination under salt stress are -0.4 MPa together with SNP; -0.4 MPa together with inactive SNP; -0.5 MPa together with SNP and -0.5 MPa together with inactive SNP. Effects similar to those brought about by the sodium nitroprusside treatment were also observed in the treatments with inactive sodium nitroprusside and ferrocyanide, indicating that the effects observed were related to release of the cyanide present in the sodium nitroprusside.

Effect of cyanide by sodium nitroprusside (SNP) application on germination, antioxidative system and lipid peroxidation of Senna macranthera seeds under saline stress

“…These data corroborates studies carried out with the same specie (Silva et al, 2018) and where these substances are acting to bring about this recovery. In Arabidopsis a weakening of the root cell wall was observed 2-4 h after salt application (Feng et al, 2018), which could be facilitating the entry of the SNP and causing a greater efficacy when applied in conjunction (the stress saline and the donor).…”

“…H. S. Irwin & Barneby, it was observed that the application of SNP in conjunction with saline stress is the best method to recover the germination of these seeds. These effects were similar to those brought about by the sodium nitroprusside treatment and were also observed in the treatments with inactive sodium nitroprusside and ferrocyanide, indicating that the effects observed were related to release of the cyanide present in the sodium nitroprusside (Silva et al, 2018).…”

“…Two concentrations of NaCl osmotic potentials (-0.4 and -0.5 MPa) were selected, causing a reduction of about 50% in seed germination (Silva et al, 2015). Saline stresses of -0.4 and -0.5 MPa were applied in conjunction with 100 μM SNP, 100 μM inactive SNP (48 h of exposure to light) and pure water (control) that are efficient in the recovery of stress (Silva et al, 2018).…”

Biology of germination of Solanum americanum: a weed ascending in Brazilian crops

“…This is because salt stress limits water uptake by seeds, so without water uptake, the germination process is reduced or inhibited (Farooq et al 2017). The results of Silva et al (2018) highlight that salt stress delays germination and that the mechanisms involved need to be clarified in order to make a decision. As it is a weed, these strategies may be useful to adopt more efficient management and reduce the establishment of this species in crops.…”