Saliva analysis by Surface-Enhanced Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (SELDI-TOF-MS) in orthodontic treatment: first pilot study

Ciavarella, Domenico; Mastrovincenzo, Mario; D’Onofrio, Valentina; Chimenti, Claudio; Parziale, Vincenzo; Barbato, Ersilia; Muzio, Lorenzo Lo

doi:10.1016/j.pio.2011.06.002

Cited by 15 publications

(12 citation statements)

References 17 publications

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“…However, this study did not measure any protein products. One study used advanced mass spectrometry that combines protein chips and mass spectrometry known as surface‐enhanced laser desorption/ionization time‐of‐flight mass spectrometry (SELDI‐TOF‐MS) was excluded because the technique used does not produced identifiable protein biomarkers . One study was excluded because it was in Italian .…”

Section: Discussionmentioning

confidence: 99%

“…One study used advanced mass spectrometry that combines protein chips and mass spectrometry known as surfaceenhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) was excluded because the technique used does not produced identifiable protein biomarkers. 31 One study was excluded because it was in Italian. 32 A study demonstrated differential salivary metabolomic profile changes in root resorption cases compared to controls.…”

Section: Discussionmentioning

confidence: 99%

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Salivary protein biomarkers associated with orthodontic tooth movement: A systematic review

Allen

Edelmann

Abdulmajeed

et al. 2019

Orthod Craniofacial Res

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Structured Abstract Objective Saliva can provide a non‐invasive approach to indicate changes in the oral and systemic conditions. Salivary proteomics allows the discovery of new protein biomarkers associated with certain conditions. The effectiveness and physiological effects of orthodontic tooth movement in theory can be measured using salivary protein biomarkers. Setting and Sample Population This study applied a systematic review to analyse current literature to define and summarize salivary biomarkers associated with orthodontic tooth movement identified by mass spectrometry proteomics and other protein detection techniques. Materials and Methods Peer‐reviewed articles published through the 15th of November 2018 via the PubMed, EMBASE, Web of Science and Dentistry & Oral Sciences databases were reviewed. Only studies analysing protein biomarkers in saliva samples collected from human subjects associated with orthodontic treatments were included. Results Out of 482 articles, 7 studies were selected. Sample size ranged from 3 to 72 subjects. Minor variations of unstimulated whole saliva sample collection protocol were noted. Mass spectrometry proteomics and ELISA represented the majority of biomarker discovery and targeting, respectively. Twenty biomarkers were identified or chosen as target biomarkers. Conclusion Salivary proteins may be used to indicate effectiveness of orthodontic treatment and orthognathic treatment as well as adverse treatment consequence, such as root resorption.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Salivary protein biomarkers associated with orthodontic tooth movement: A systematic review

Allen

Edelmann

Abdulmajeed

et al. 2019

Orthod Craniofacial Res

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show abstract

“…Surface-Enhanced Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (SELDI-TOF-MS) can get stable profile of salivary proteome of healthy controls [23]. It could be also used in detecting the differences between pre- and post-orthodontic treatment [24] or in high-throughput breast cancer biomarker discovery [25]. Two-dimensional gel electrophoresis (2DE) combined with MS performed well in lung cancer [26] and breast cancer [20] biomarker detection with high sensitivity and specificity.…”

Section: Biomarkers In Salivamentioning

confidence: 99%

Salivary biomarkers in cancer detection

2016

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Cancer is the second most common cause of death in the United States. Its symptoms are often not specific and absent, until the tumors have already metastasized. Therefore, there is an urgent demand for developing rapid, highly accurate and non-invasive tools for cancer screening, early detection, diagnostics, staging and prognostics. Saliva as a multi-constituent oral fluid, comprises secretions from the major and minor salivary glands, extensively supplied by blood. Molecules such as DNAs, RNAs, proteins, metabolites, and microbiota, present in blood, could be also found in saliva. Recently, salivary diagnostics has drawn significant attention for the detection of specific biomarkers, since the sample collection and processing are simple, cost-effective, precise and do not cause patient discomfort. Here, we review recent salivary candidate biomarkers for systemic cancers by dividing them according to their origin into: genomic, transcriptomic, proteomic, metabolomic and microbial types.

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“…Previous studies have employed the SELDI technique to search for potential biomarkers in the salivary proteome (3,6,(34)(35)(36)(37)(38)(39)(40)(41)(42)(43)(44)(45)(46)(47)(48) (Table I).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies on the saliva proteome using SELDI technology have evaluated the modifications affecting the oral mucosa and bones in patients undergoing orthodontic treatment (47). In addition, a previous study employed SELDI-TOF/MS to investigate the influence of sodium chloride and sucrose solutions on the presence of proteins of 2-20 kDa in whole saliva (48).…”

Section: Discussionmentioning

confidence: 99%

Novel possibilities in the study of the salivary proteomic profile using SELDI-TOF/MS technology

et al. 2016

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Abstract. There is currently an increasing interest in exploring human saliva to identify salivary diagnostic and prognostic biomarkers, since the collection of saliva is rapid, non-invasive and stress-free. Diagnostic tests on saliva are common and cost-effective, particularly for patients who need to monitor their hormone levels or the effectiveness of undergoing therapies. Furthermore, salivary diagnostics is ideal for surveillance studies and in situations where fast results and inexpensive technologies are required. The most important constituents of saliva are proteins, the expression levels of which may be modified due to variations of the cellular conditions. Therefore, the different profile of proteins detected in saliva, including their absence, presence or altered levels, is a potential biomarker of certain physiological and/or pathological conditions. A promising novel approach to study saliva is the global analysis of salivary proteins using proteomic techniques. In the present study, surface-enhanced laser desorption/ionization-time-of-flight/mass spectrometry (SELDI-TOF/MS), one of the most recent proteomic tools for the identification of novel biomarkers, is reviewed. In addition, the possible use of this technique in salivary proteomic studies is discussed, since SELDI technology combines the precision of matrix-assisted laser desorption/ionization-TOF/MS proteomic analysis and the high-throughput nature of protein array analysis.

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Saliva analysis by Surface-Enhanced Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (SELDI-TOF-MS) in orthodontic treatment: first pilot study

Cited by 15 publications

References 17 publications

Salivary protein biomarkers associated with orthodontic tooth movement: A systematic review

Salivary protein biomarkers associated with orthodontic tooth movement: A systematic review

Salivary biomarkers in cancer detection

Novel possibilities in the study of the salivary proteomic profile using SELDI-TOF/MS technology

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