Saliva biomarker detection using an aptamer-based nanosensor

Zhang, Wenjun; Nazarian, Sheyda; Wang, Ming L.

doi:10.1117/12.2298115

Cited by 1 publication

(1 citation statement)

References 63 publications

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“…DNA aptamer against CEA: 5'-NH2-GAC GAT AGC GGT GAC GGC ACA GAC GTC CCG CAT CCT CCG-3' (Mw 12,143 g mol−1) [21] was synthesized and purified from AllBio Science Inc., Taiwan. CEA antigens (ab742) and Anti-CEA antibodies (ab131070) were purchased from Abcam.…”

Section: Methodsmentioning

confidence: 99%

A Graphene-PEDOT:PSS Modified Paper-Based Aptasensor for Electrochemical Impedance Spectroscopy Detection of Tumor Marker

Yen

Chao

Yeh

2020

Sensors

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A graphene and poly (3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) modified conductive paper-based electrochemical impedance spectroscopy (EIS) aptasensor has been successfully fabricated by a simple and continuous coating process. A graphene/PEDOT:PSS modified paper electrode forms the nanocomposite providing a conductive and sensitive substrate for further aptamer functionalization of the biosensor. This low-cost paper-based aptasensor exhibits its sensitivity to carcinoembryonic antigens (CEA) in standard buffer solutions and human serum samples in a linear range of 0.77–14 ng·mL−1. The limit of detection (LOD) is found to be 0.45 ng·mL−1 and 1.06 ng·mL−1 for CEA in both samples, separately. This aptamer-based sensing device was also evaluated and received a good correlation with the immunoassay detection method. The proposed paper-based aptasensor has demonstrated its potential as a rapid simple point-of-care analytical platform for early cancer diagnosis in less developed areas where manufacturing facilities, analytical instruments, and trained specialists are limited.

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Section: Methodsmentioning

confidence: 99%