Saliva proteomics as an emerging, non-invasive tool to study livestock physiology, nutrition and diseases

Lamy, Elsa; Mau, Marcus

doi:10.1016/j.jprot.2012.05.007

Cited by 93 publications

(74 citation statements)

References 88 publications

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“…PIP/GCDFP-15 has been reported to be expressed in rat salivary glands (Mirels et al, 1998). Expression of GCDFP-15/PIP homologue was also reported in mouse salivary gland, although its function is still doubtful (Lamy et al, 2010;Lamy and Mau, 2012), and the expression PIP homologue was also reported in Bos taurus cattle (Zimin et al, 2009). In this present study, PIP homologue was observed in the saliva of Bali cattle (Bos sondaicus or Bos javanicus).…”

Section: Resultssupporting

confidence: 58%

“…Consequently, besides supplying its components involved in the process of food digestion, saliva also plays an important role in maintaining the body's immunity by providing multiple immunogenic components (Malamud et al, 2011). In addition, saliva can also be used as a diagnostic medium for non-invasive examination of the animal condition (Kaufman and Lamster, 2002;Lamy and Mau, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Studies on saliva have been conducted much more on human saliva, while they have just recently begun to develop on ruminants (Beauchemin et al, 2008;Lamy et al, 2009Lamy et al, , 2010Wheeler et al, 1998Wheeler et al, , 2002Wheeler et al, , 2007Wheeler et al, , 2011Lamy and Mau, 2012). There are many different types of proteins in the ruminant saliva with quite a large range of molecular mass reported elsewhere (Lamy et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Proteomic Analysis of a 14.2 kDa Protein Isolated from Bali Cattle(Bos Sondaicus/Javanicus)Saliva Using Single Dimension Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis and Matrix-Assisted Laser Desorption Ionization Time-of-Flight Tandem Mass Spectrometer

Depamede

2013

Italian Journal of Animal Science

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A 14.2 kDa protein isolated from Bali cattle (Bos sondaicus/javanicus) saliva has been reported to have a bactericidal activity. The aim of this study was to analyse the nature of a 14.2 kDa protein using single dimension (1-D) sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and matrix-assisted laser desorption ionization time-of-flight tandem mass spectrometer (MALDI TOF/TOF). The protein was isolated by means of polyethylene glycol (PEG)/sodium sulfate aqueous twophase system, and then determined by 12.5% SDS-PAGE. A band of 14.2 kDa was sliced and analysed by MALDI TOF/TOF mass spectrometer using a 5800 proteomics analyzer. Mascot search and National Center for Biotechnology Information (NCBI) Blast search revealed that the spot of 1-D SDS-PAGE consisted of three proteins: zymogen granule protein 16 homologue B, pancreatic adenocarcinoma upregulated factor-like, and prolactin-inducible protein homologue precursor. The three proteins have been observed in Bos taurus and other species such as mouse. The actual nature of the proteins and their function in Bali cattle (Bos sondaicus/javanicus), as well as the connection with the evolution of bovines need further analysis.

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Section: Resultssupporting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Proteomic Analysis of a 14.2 kDa Protein Isolated from Bali Cattle(Bos Sondaicus/Javanicus)Saliva Using Single Dimension Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis and Matrix-Assisted Laser Desorption Ionization Time-of-Flight Tandem Mass Spectrometer

Depamede

2013

Italian Journal of Animal Science

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“…Although advances have emerged, in the last years, for some animal species, salivary proteins identification continues to be challenging, due to the lack of complete and annotated genome and protein sequences [81]. When this happens, the search in other related species databases is needed, increasing the possibility of to rise the number of false positive results.…”

Section: Methodological Issues Related To the Use Of Electrophoresis mentioning

confidence: 99%

The Use of Electrophoresis for the Study of Saliva Involvement in Ingestive Behavior

Lamy¹,

Morzel²,

Rodrigues³

et al. 2015

Field Effect Electroosmosis - A Novel Phenomenon in Electrokinetics and Its Applications in Capillary Electrophoresis

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“…Fisiologia comparada do fluído... BARBOSA JÚNIOR, S. A.; BAPTISTA, R. A. de A.; BARBOSA, C. N. clínica apresenta um baixo risco ocupacional para os profissionais de saúde durante sua coleta e processamento (FERRI, 1998;KAUFMAN;LAMSTER, 2002;AMADO et al, 2006;MOURA et al, 2007;LAMY;MAU, 2012;BADIYANI;KUMAR;MARU, 2013), mostrando ser uma ferramenta que traz praticidade e diminui o estresse provocado pela coleta do sangue, principalmente em animais de produção, favorecendo o bem-estar animal (PRICKETT; ZIMMERMAN, 2010;BARBOSA et al, 2013). Devido a essas qualidades, observa-se uma crescente utilização do FO em substituição ao sangue como amostra clínica, tanto nos seres humanos, quanto nos animais.…”

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Fisiologia Comparada Do Fluido Oral E Suas Principais Aplicações Clínicas

Júnior

Baptista

Barbosa

2016

ArqVet

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BARBOSA JÚNIOR, S. A.; BAPTISTA, R. I. A. de A.; BARBOSA, C. N. Fisiologia comparada do fluído oral e suas principais aplicações clínicas. Arq. Ciênc. Vet. Zool. UNIPAR, Umuarama, v. 18, n. 4, p. 259-268, out./dez. 2015. RESUMO:O fluido oral é um líquido incolor e viscoso que resulta da combinação entre a saliva e os transudatos da cavidade oral, transudato da mucosa oral e fluido crevicular gengival. A saliva é secretada pelas glândulas salivares, enquanto o transudatos da cavidade oral tem origem nos capilares da mucosa e nos tecidos gengivais. O uso do fluido oral é assegurando por uma ampla base de investigações científicas na medicina humana e veterinária. Vários patógenos podem ser avaliados pela reatividade de anticorpos e ou identificação de antígenos. Além disso, o procedimento de coleta é simples, não invasivo e garante uma amostragem sistemática nos diagnósticos clínicos e em pesquisas. Por conseguinte, as informações relacionadas à secreção e composição, bem como os mecanismos de transporte dos componentes do fluido oral são de grande valor. Objetivou-se com este trabalho realizar uma revisão de literatura sobre o fluido oral, enfatizando o processo de secreção, composição, mecanismos de transporte de substâncias, bem como suas aplicações clínicas e principais limitações nos seres humanos e nos animais domésticos. PALAVRAS-CHAVE: Bem estar. Diagnóstico. Saliva. COMPARATIVE PHYSIOLOGY OF FLUID ORAL AND MAIN CLINICAL APPLICATIONS ABSTRACT:The oral fluid is a colorless and viscous liquid that results from the combination of saliva and transudates of oral cavity, oral mucosal transudate and gingival crevicular fluid. Saliva is secreted from the salivary glands, while the transudates of oral cavity as its origin in capillary mucosa and gingival tissues. The use of oral fluid is thereby ensuring a broad base of scientific research in human and veterinary medicine. Several pathogens can be assessed by reactivity or identification of antibodies and antigens. Furthermore, the collection procedure is simple, noninvasive and provides a systematic sampling in clinical diagnostics and in research. Therefore, the information related to the secretion and composition as well as the transport mechanisms of the oral fluid components are of great value. The aim of this work is make a critical review of the oral fluid, emphasizing the process of secretion and composition, transport mechanisms of the substances present in the plasma to the oral cavity and its clinical applications and major limitations in humans and domestic animals. KEYWORDS: Diagnostic. Saliva. Welfare. FISIOLOGÍA COMPARADA DEL FLUIDO ORAL Y SUS PRINCIPALES APLICACIONES CLÍNICAS RESUMEN:El fluido oral es un líquido incoloro y viscoso que resulta de la combinación entre la saliva y los trasudados de la cavidad oral, trasudado de la mucosa oral y fluido crevicular gingival. La saliva es secretada por las glándulas salivales, mientras el trasudado de la cavidad oral tiene origen en los capilares de la mucosa y en los tejidos gingivales. El uso del fluido ...

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Saliva proteomics as an emerging, non-invasive tool to study livestock physiology, nutrition and diseases

Cited by 93 publications

References 88 publications

Proteomic Analysis of a 14.2 kDa Protein Isolated from Bali Cattle(Bos Sondaicus/Javanicus)Saliva Using Single Dimension Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis and Matrix-Assisted Laser Desorption Ionization Time-of-Flight Tandem Mass Spectrometer

Proteomic Analysis of a 14.2 kDa Protein Isolated from Bali Cattle(Bos Sondaicus/Javanicus)Saliva Using Single Dimension Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis and Matrix-Assisted Laser Desorption Ionization Time-of-Flight Tandem Mass Spectrometer

The Use of Electrophoresis for the Study of Saliva Involvement in Ingestive Behavior

Fisiologia Comparada Do Fluido Oral E Suas Principais Aplicações Clínicas

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