Saliva specimen: A new laboratory tool for diagnostic and basic investigation

Chiappin, Silvia; Antonelli, Giorgia; Gatti, Rosalba; Palo, Elio Franco De

doi:10.1016/j.cca.2007.04.011

Cited by 704 publications

(658 citation statements)

References 85 publications

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“…It is therefore suitable for monitoring of general health and disease in children, elderly people, and non-collaborative subjects. Salivary analysis is very attractive due to the possibility of repeated sampling and longitudinal monitoring [22,25]. To use the potential of salivary oxidative stress markers for diagnostics of oral diseases, various criteria must be optimized.…”

Section: Introductionmentioning

confidence: 99%

Salivary Markers of Oxidative Stress and Antioxidant Status: Influence of External Factors

2013

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Abstract.BACKGROUND: Salivary markers of oxidative stress and antioxidant status represent promising tool for the research of oral diseases. One of the criteria is the validation of these biomarkers from the perspective of the confounding and modifying factors. AIM: To examine the effect of circadian rhythm, tooth-brushing and ascorbic acid treatment on selected salivary markers of oxidative and carbonyl stress, and antioxidant status. SUBJECTS AND METHODS: Whole unstimulated saliva samples were collected from 19 healthy participants three times during a day, before and after tooth-brushing, and before and after the administration of vitamin C (250 mg). Advanced oxidation protein products (AOPP), thiobarbituric acid reactive substances (TBARS), advanced glycation end products (AGEs), ferric reducing antioxidant power (FRAP) and total antioxidant capacity (TAC) were measured. RESULTS: Salivary AGEs levels varied significantly during the day (p < 0.05) with the highest concentrations in the morning. FRAP levels varied during the day (p < 0.01) with the highest concentrations in the afternoon. Tooth-brushing decreased AGEs (p < 0.05) and TBARS levels (p < 0.01) and increased FRAP levels (p < 0.05). Single intake of vitamin C significantly decreased AGEs (p < 0.001) and increased both FRAP (p < 0.01) and TAC (p < 0.01) concentrations. CONCLUSION: Significant daily variations were observed in salivary AGEs and FRAP levels. Tooth-brushing and treatment with vitamin C decreased carbonyl stress and increased the antioxidant status. These results are important from the perspective of using saliva for the research of oral diseases.

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Section: Introductionmentioning

confidence: 99%

Salivary Markers of Oxidative Stress and Antioxidant Status: Influence of External Factors

2013

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“…The assay of saliva specimens is an increasing area of research with implications for basic and clinical purposes and saliva analysis provides important information about the functioning of various organs within the body (1). Saliva can also be collected through a simple and noninvasive method without medical supervision.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous quantitative analysis of salivary cortisol and cortisone in Korean adults using LC-MS/MS

Lee¹,

Kwon²,

Shin³

et al. 2010

BMB Reports

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The levels of salivary cortisol and cortisone in Korean adults were measured for the first time using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The salivary cortisol and cortisone were separated within 10 min. The regression coefficients (r) of the calibration curves were greater than 0.999 for the two steroids. The limits of quantitation (LOQ) were 0.2 ng/ml for cortisol and 1 ng/ml for cortisone. The intra-day precisions of the assay were ＜3.9% and 8.6% for cortisol and cortisone respectively, and the inter-day precisions were ＜1.9% and 4.3% for cortisol and cortisone, respectively. The salivary cortisone concentrations were approximately 4-9 times higher than those of salivary cortisol during the daytime. Diurnal rhythms, during which the cortisol and cortisone concentrations were higher in the morning than in the afternoon, were also observed. The present assay may be useful for the diagnosis of several adrenal dysfunctions in clinical biochemistry. [BMB reports 2010; 43(7): 506-511]

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“…For example, by employing the biotinylated NaSmF 4 and Sr 2 YF 7 :Eu 3+ NPs as the DELBA nanoprobes, we achieved LODs of ≈6.0 fM and ≈95 pM for CEA, respectively (Figures 8c and 8d). [[qv: 20a]] More recently, benefiting from the high sensitivity of DELBA, we realized the detection of CEA in saliva, in which the CEA level is much lower than in serum 33. The salivary diagnostics of tumor markers provides a more convenient and non‐invasive sampling strategy than conventional serum‐based assays, therefore is highly promising for cancer screening and monitoring in future family and community medical service.…”

Section: Ultrasensitive In Vitro Detection Of Tumor Markersmentioning

confidence: 99%

Ultrasensitive Luminescent In Vitro Detection for Tumor Markers Based on Inorganic Lanthanide Nano‐Bioprobes

Zheng

Zhou

et al. 2016

Advanced Science

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Ultrasensitive and accurate detection of tumor markers is of vital importance for the screening or diagnosis of cancers at their early stages and for monitoring cancer relapse after surgical resection. Inorganic lanthanide (Ln3+) nanoparticles (NPs), owing to their superior physicochemical characteristics, are regarded as a new generation of luminescent nano‐bioprobes in the field of cancer diagnosis and therapy. In this progress report, a focus is set on our recent efforts on the development of inorganic Ln3+‐NPs as efficient luminescent nano‐bioprobes for the ultrasensitive in vitro biodetection of tumor markers, with an emphasis on the dissolution‐enhanced luminescent bioassay (DELBA), an emerging technique recently developed toward practical medical applications.

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Saliva specimen: A new laboratory tool for diagnostic and basic investigation

Cited by 704 publications

References 85 publications

Salivary Markers of Oxidative Stress and Antioxidant Status: Influence of External Factors

Salivary Markers of Oxidative Stress and Antioxidant Status: Influence of External Factors

Simultaneous quantitative analysis of salivary cortisol and cortisone in Korean adults using LC-MS/MS

Ultrasensitive Luminescent In Vitro Detection for Tumor Markers Based on Inorganic Lanthanide Nano‐Bioprobes

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