SALMONELLA | Detection by Enzyme Immunoassays

Patel, Pradip D.

doi:10.1006/rwfm.1999.1390

Cited by 3 publications

(3 citation statements)

References 14 publications

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“…1993;Andrews et al 1995Andrews et al , 2001. As an alternative to culture methods, immunological methods in different formats have been introduced for the rapid detection of Salmonella (Patel 2000;Fukuda et al 2005). Immunological methods depend on binding of a specific antibody to an antigen on the bacterium.…”

Section: Introductionmentioning

confidence: 99%

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Adding a selective enrichment step to the iQ-CheckTM real-time PCR improves the detection of Salmonella in naturally contaminated retail turkey meat products

Fakhr¹,

McEvoy²,

Sherwood³

2006

Lett Appl Microbiol

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Aims: The aim of this study was to compare the real‐time iQ‐CheckTMSalmonella kit (Bio‐Rad) with the immunocapture assay RapidCheck®Salmonella method, and a conventional culture method (FSIS, USDA) in detecting Salmonella in naturally contaminated turkey meat products. This study was also designed to determine if a selective enrichment step might improve the real‐time detection of Salmonella. Methods and Results: Using the culture method, Salmonella was recovered from 49 out of 99 retail turkey meat samples collected. RapidCheck® failed to detect 11 Salmonella samples that were positive by the culture method. The iQ‐CheckTM real‐time PCR also failed to detect three samples that were positive by the culture method. However, when carried out after a selective enrichment step, the iQ‐CheckTM real‐time PCR detected all 49 Salmonella samples recovered by the culture method. The iQ‐CheckTM real‐time PCR detected the presence of Salmonella in some samples that were not recovered by the culture method. Conclusions: Adding a selective enrichment step to the iQ‐CheckTM real‐time PCR improves the detection of Salmonella in naturally contaminated turkey meat samples. Significance and Impact of the Study: The iQ‐CheckTMSalmonella real‐time PCR can be used as a rapid method to monitor Salmonella in turkey meat, together with conventional culture methodology.

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Section: Introductionmentioning

confidence: 99%

“…1995, 2001). As an alternative to culture methods, immunological methods in different formats have been introduced for the rapid detection of Salmonella (Patel 2000; Fukuda et al. 2005).…”

Section: Introductionmentioning

confidence: 99%

Adding a selective enrichment step to the iQ-CheckTM real-time PCR improves the detection of Salmonella in naturally contaminated retail turkey meat products

Fakhr¹,

McEvoy²,

Sherwood³

2006

Lett Appl Microbiol

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“…). As an alternative to culture methods, immunological methods in different formats have been introduced for rapid detection of Salmonella (Patel 2000). Vidas is an automated ELISA method based on the detection of Salmonella using specific antibodies coated on the inner surface of a tip-like disposable pipette which is introduced into the Vidas system along with the Vidas Salmonella strip containing the boiled Salmonella culture (Curiale et al 1997;Keith 1997).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of real-time PCR vs automated ELISA and a conventional culture method using a semi-solid medium for detection of Salmonella

Uyttendaele¹,

Vanwildemeersch²,

Debevere³

2003

Lett Appl Microbiol

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Aims: Evaluation of iQ-Check PCR Salmonella for Salmonella detection in artificially and naturally contaminated food and environmental field samples. Methods and Results: Artificially contaminated samples (poultry meat and ground red meat) subjected to cold-and freeze-stress, and 120 naturally contaminated samples (swabs and meat) were tested for Salmonella using the diagnostic semi-solid Salmonella medium (DIASALM) method, the Vidas assay and the iQ-Check PCR assay after 24 h enrichment in buffered peptone water. 2Conclusions: Both the iQ-Check PCR and the Vidas assay provide a rapid and user friendly screening method for detection of Salmonella. False negative samples were obtained for the inoculated samples using both the iQ-Check PCR assay and the Vidas method when Salmonella cells were severely stressed. In total 45 of 120 naturally contaminated field samples showed Salmonella positive using the DIASALM method. The agreement percentage with the DIASALM method was respectively 92% for the iQ-Check PCR and 95% for the Vidas method. Significance and Impact of the Study: False-negative samples were obtained for the inoculated samples using both the iQ-Check PCR assay and the Vidas method when Salmonella cells were severely stressed, e.g. freezing at )18°C for 7 days. Of the 120 naturally contaminated field samples 45 showed Salmonella positive using the DIASALM method. The agreement percentage with the DIASALM method was 92% for the iQ-Check PCR and 95% for the Vidas method respectively.

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Prevalence of Salmonella in a Poultry Slaughterhouse

Reiter

Fiorese

Moretto

et al. 2007

Journal of Food Protection

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The prevalence of Salmonella on surfaces, water, and broiler chicken (carcasses, parts, viscera, and spoils) taken from a poultry slaughterhouse located in the south of Brazil was studied. The automated mini-VIDAS system (a variation of the basic enzyme-linked immunosorbent assay) was used to screen for the presence of this microorganism in 615 samples, and the traditional culture method was used in 470 samples. We detected Salmonella in the following sampling points by the VIDAS Salmonella test: transport cages (16.7%), boxes (10%), scalding water (16.7%), chilled water (6.7%), carcass before evisceration (6.7%), carcass after chilling (3.3%), fresh breast (3.3%), fresh leg (10%), frozen wing (13.3%), frozen leg (13.3%), intestines (6.7%), skin of breast and leg (10%), and skin of neck (6.7%). Nevertheless, with the traditional culture method we only detected salmonellae in the following sampling points: scalding water (10%), fresh leg (6.7%), frozen wing (10%), skin of breast and leg (20%), and skin of neck (10%). Finally, 5.4% (33 of 615) of the samples analyzed by the VIDAS Salmonella system were positive, whereas the positive percentage with the traditional method was 2.6% (12 of 470). The results showed that transport cages, scalding water, frozen wing, frozen leg, and skin of breast and leg were the sampling points that demonstrated the greatest prevalence.

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SALMONELLA | Detection by Enzyme Immunoassays

Cited by 3 publications

References 14 publications

Adding a selective enrichment step to the iQ-CheckTM real-time PCR improves the detection of Salmonella in naturally contaminated retail turkey meat products

Adding a selective enrichment step to the iQ-CheckTM real-time PCR improves the detection of Salmonella in naturally contaminated retail turkey meat products

Evaluation of real-time PCR vs automated ELISA and a conventional culture method using a semi-solid medium for detection of Salmonella

Prevalence of Salmonella in a Poultry Slaughterhouse

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