Salmonella enterica subsp. enterica producing VIM-1 carbapenemase isolated from livestock farms

Fischer, Jennie; Rodríguez, Irene; Schmoger, Silvia; Friese, Anika; Roesler, Uwe; Helmuth, Reiner; Guerra, Beatriz

doi:10.1093/jac/dks393

Cited by 131 publications

(113 citation statements)

References 9 publications

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“…The first reported instance of a Salmonella isolate encoding carbapenemases (VIM-1) originated from poultry on one of the farms (G1). This isolate also encoded AmpC (40).…”

Section: Discussionmentioning

confidence: 91%

Longitudinal Monitoring of Extended-Spectrum-Beta-Lactamase/AmpC-Producing Escherichia coli at German Broiler Chicken Fattening Farms

Laube¹,

Friese²,

Salviati³

et al. 2013

Appl Environ Microbiol

133

106

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Antimicrobial resistance of Escherichia coli to modern beta-lactam antibiotics due to the production of extended-spectrum betalactamases (ESBL) and/or plasmid-mediated AmpC beta-lactamases (AmpC) represents an emerging and increasing resistance problem that dramatically limits therapeutic options in both human and veterinary medicine. The presence of ESBL/AmpC genes in commensal E. coli from food-producing animals like broilers may pose a human health hazard. However, there are no data available concerning the prevalence of ESBL/AmpC-producing E. coli in German broiler flocks using selective methods. In this longitudinal study, samples were taken from seven conventional broiler fattening farms at three different times within one fattening period. Various samples originating from the animals as well as from their direct environment in the barn were investigated for the occurrence of ESBL/AmpC-producing E. coli. Average detection levels of 51, 75, and 76% in animal samples collected during the three samplings in the course of the fattening period demonstrate a colonization of even 1-day-old chicks, as well as a continuous significant (P < 0.001) increase in prevalence thereafter. The detection frequencies in housing environmental samples were relatively high, with an increase over time, and ranged between 54.2 and 100%. A total of 359 E. coli isolates were characterized by PCR and partly via the disc diffusion method. This study shows that prevalence of ESBL/AmpC-producing E. coli increases during the fattening period of the broiler flocks examined. Both colonized day-old chicks and contaminated farm environments could represent significant sources of ESBL/AmpC-producing E. coli in German broiler fattening farms.

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“…The first reported instance of a Salmonella isolate encoding carbapenemases (VIM-1) originated from poultry on one of the farms (G1). This isolate also encoded AmpC (40).…”

Section: Discussionmentioning

confidence: 91%

Longitudinal Monitoring of Extended-Spectrum-Beta-Lactamase/AmpC-Producing Escherichia coli at German Broiler Chicken Fattening Farms

Laube¹,

Friese²,

Salviati³

et al. 2013

Appl Environ Microbiol

133

106

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“…In recent years, a few clinical cases have been reported, all of them corresponding to NDM-producing Salmonella (10,11,12). Interestingly, VIM alleles were not reported in Salmonella isolates until 2013 (9,13).…”

mentioning

confidence: 99%

Synthesis of Metallo-β-Lactamase VIM-2 Is Associated with a Fitness Reduction in Salmonella enterica Serovar Typhimurium

Cordeiro

Chabalgoity

Yim

et al. 2014

Antimicrob Agents Chemother

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Antibiotic resistance, especially due to ␤-lactamases, has become one of the main obstacles in the correct treatment of Salmonella infections; furthermore, antibiotic resistance determines a gain of function that may encompass a biological cost, or fitness reduction, to the resistant bacteria. The aim of this work was to determine in vitro if the production of the class B ␤-lactamase VIM-2 determined a fitness cost for Salmonella enterica serovar Typhimurium. To that end the gene bla VIM-2 was cloned into the virulent strain S. Typhimurium SL1344, using both the tightly regulated pBAD22 vector and the natural plasmid pST12, for inducible and constitutive expression, respectively. Fitness studies were performed by means of motility, growth rate, invasiveness in epithelial cells, and plasmid stability. The expression of bla VIM-2 was accompanied by alterations in micro-and macroscopic morphology and reduced growth rate and motility, as well as diminished invasiveness in epithelial cells. These results suggest that VIM-2 production entails a substantial fitness cost for S. Typhimurium, which in turn may account for the extremely low number of reports of metallo-␤-lactamase-producing Salmonella spp.

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“…Apart from the occurrence of ESBL producers, some recent studies identified carbapenemases in Gram-negative bacteria from animals, i.e., OXA-23-producing Acinetobacter genomospecies 15TU from dairy cattle in France (9), OXA-23-producing Acinetobacter spp. from horses in Belgium (10), and VIM-1-producing E. coli isolates from pigs and poultry in Germany (11,12). Our study aimed to evaluate the occurrence of ESBL-producing or carbapenemase-producing Enterobacteriaceae as commensals (rectal isolates) or as pathogens (urinary tract infections) among companion animals living in France.…”

mentioning

confidence: 99%

Extended-Spectrum β-Lactamase CTX-M-15-Producing Klebsiella pneumoniae of Sequence Type ST274 in Companion Animals

Poirel

Nordmann

Ducroz

et al. 2013

Antimicrob Agents Chemother

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Screening of extended-spectrum ␤-lactamase (ESBL)-producing Gram-negative bacteria in companion animals living in the Paris area in France identified a high rate of CTX-M-15-producing Klebsiella pneumoniae. Those isolates were recovered during the 2010-2011 period from both infections and asymptomatic colonizations. Sequence typing revealed that most of these isolates belonged to sequence type ST274. Interestingly, the bla CTX-M-15 gene was located on a specific and novel plasmid scaffold. These findings highlight that companion animals may be reservoirs for CTX-M-15-producing K. pneumoniae evolving separately from the human reservoir of CTX-M-15 producers.

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Salmonella enterica subsp. enterica producing VIM-1 carbapenemase isolated from livestock farms

Cited by 131 publications

References 9 publications

Longitudinal Monitoring of Extended-Spectrum-Beta-Lactamase/AmpC-Producing Escherichia coli at German Broiler Chicken Fattening Farms

Longitudinal Monitoring of Extended-Spectrum-Beta-Lactamase/AmpC-Producing Escherichia coli at German Broiler Chicken Fattening Farms

Synthesis of Metallo-β-Lactamase VIM-2 Is Associated with a Fitness Reduction in Salmonella enterica Serovar Typhimurium

Extended-Spectrum β-Lactamase CTX-M-15-Producing Klebsiella pneumoniae of Sequence Type ST274 in Companion Animals

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