Salmonella isolation with Rappaport–Vassiliadis enrichment medium seeded with different sized inocula of pre-enrichment cultures of meat products and sewage polluted water

Abstract

SUMMARYA total of 574 samples, of seven different types, were examined for the presence of salmonellas. All the specimens were pre-enriched in buffered peptone water and enriched in Rappaport-Vassiliadis medium (RV medium). In one trial 01 ml of pre-enrichment culture of 497 samples (79 chicken carcasses, 228 specimens of minced meat, 100 pork sausages, 19 samples of dried powdered chicken meat, 11 specimens of faeces of healthy pigs and 60 samples of sewage polluted natural sea water) was seeded to 10 ml as well as to 100 ml of RV medium. With the first inoculum (ratio 01::10), 111 samples were found to contain salmonellas, while with the second inoculum (ratio 01: 100), only 102 positive samples were detected. This difference is marginally significant (P < 0 05). In another trial, 0 1 ml, 0-2 ml and 0.5 ml of pre-enrichment culture of 162 specimens (71 chicken carcasses, 40 samples of sewage polluted sea water and 51 samples of sewage polluted river water) were in turn introduced to 10 ml of RV medium. With the 0.1 ml inoculum 93 positive samples were detected, while with the 0-2 and 0 5 ml inocula 93 and 88 positive samples were found. The differences are not statistically significant. In these trials the growth of competing organisms was minimal with ratios of inocula 01: 10 or 0-1:100.

Correlation of bacterial indicator organisms with Salmonella spp., Staphylococcus aureus and Candida albicans in sea water

“…Campylobacter analyses were conducted using enrichment in Preston broth and subculture on Preston Medium (Oxoid). The detection of salmonellas was performed according to the WHO guidelines and Vassiliadis et al (1985). Identification of salmonellae was carried out by appropriate biochemical and serological tests in the National Salmonella-Shigella Reference Centre, Greece.…”

The isolation of salmonellas and campylobacters

“…For Rappaport and RV media, however, a much smaller inoculum is favoured by most workers. The use of a 1 : 100 or 1 : 200 ratio has been recommended (Vassiliadis 1983;Vassiliadis et al 1985;Fricker 1984aFricker et al 1985) although workers have reported better results using 1 : 20 (Harvey & Price 1983) or 1 : 1000 (Tongpim et al 1984) with RV medium. Inoculation ratios of 1 : lo00 and 1 : 2000 have been recommended for Rappaport's medium incubated at 37°C (Rappaport et al 1956;Harvey et al 1979;Harvey & Price 1980, 1981.…”

Kan Donörlerinde HBsAg, Anti-HCV, Anti-HIV, Sifiliz Seroprevalansı ve Macro-EL ISA Sonuçlarının Optik Dansite Değerleri ile Doğrulama Testlerinin Karşılaştırılması

“…(15) ilk olarak 1976'da Rappaport'un Salmonella'lar için MgCl 2-malaşit yeşili zenginleştirme besiyerine iki önemli modifikasyon getirdiklerinden beri diğer birçok araştırmacı besiyerinin dinamik ve kinetiğini araştırmış ve ek modifikasyonlar içeren besiyerlerinin verimliliğini karşılaştırmıştır. Bu ve buna benzer çalışmalar RV besiyerinin Salmonella'lar için (Salmonella serotip Typhi hariç) geleneksel zenginleştirme besiyerleri olan tetratiyonat ve selenit besiyerlerinden üstünlüğünü göstermiştir (15)(16)(17)(18)(19) . (20) .…”

“…İncelenecek örnekler, steril şartlarda küçük parçalara ayrılmış ve 25 g örnek 225 ml % 1'lik peptonlu su (ph:7,2) içinde 37°C'de 18-24 saat tutularak ön zenginleştirmesi yapılmıştır. Daha sonra her örnekten 0,1 ml alarak üç farklı formülde hazırlanmış ve steril 10 ml Rappaport-Vassiliadis zenginleştirici besiyerine ekilmiştir (14,15) . Rappaport-Vassiliadis zenginleştirici besiyerinin birincisi orijinal formüle (RV) göre hazırlanmış ve pH'sı beş olarak ayarlanmıştır.…”

“…Rappaport-Vassiliadis zenginleştirici besiyerinin birincisi orijinal formüle (RV) göre hazırlanmış ve pH'sı beş olarak ayarlanmıştır. İkincisi (RVJ), Chaiwat Jatisatienr'a göre modifiye edilmiş olup (pH'sı 7), tripton yerine soya peptonu ve maya ekstraktı içermektedir (15)…”