Salmonella surveillance with reference to pigs – Cardiff abattoir, 1968–1975

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SUMMARYSix hundred and eighty three samples of chicken giblets were examined for salmonellas. Three hundred and forty nine of these were neck and crop specimens and 334 were combined liver and heart samples. Two hundred and ten, in all, contained salmonellas.The technique of examination included pre-enrichment in buffered peptone water at 37°C for 18 h and subculture to three enrichment media: MullerKauffmann tetrathionate, selenite F and Rappaport's magnesium chloride malachite green broth. Inocula from buffered peptone water to 10 ml of tetrathionate and selenite were 1 ml in each case. The inoculum from the pre-enrichment medium to 10 ml of Rappaport was 0 005 ml. Tetrathionate and selenite were incubated at 43°C for 48 h. Rappaport's medium was incubated at 37°C for 48 h. Subcultures from all three enrichment broths were made at 24 h and 48 h to brilliant green MacConkey agar. Selective agars were incubated at 37°C for 24 h.The most successful technique for salmonella isolation used Rappaport's medium, which was significantly more efficient than either tetrathionate or selenite. This finding reinforces results obtained using sewage polluted natural water as test material and it is suggested that routine examination of environment samples for salmonellas could be based on Rappaport's medium alone.If S. typhi, S. dublin or subgenus III salmonellas were likely to be present in the sample, the technique described here would require modification.

Section: Resultsmentioning

confidence: 85%

Section: Resultsmentioning

confidence: 99%

Comparison of selenite F, Muller-Kauffmann tetrathionate and Rappaport's medium for salmonella isolation from chicken giblets after pre-enrichment in buffered peptone water

Harvey

Price

1981

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“…at slaughter (Riley 1970;Edel & Kampelmacher 1976;Harvey, Price & Morgan 1977). These studies have shown that the intestinal tract and mesenteric lymph nodes are frequently infected and provide a source from which salmonella may be spread in the abattoir during evisceration and dressing.…”

Section: Introductionmentioning

confidence: 99%

The effect of time in lairage on the frequency of salmonella infection in slaughtered pigs

Craven

Hurst

1982

SUMMARYGroups of pigs were brought to an abattoir by truck and approximately 25 were killed on each of the next 3 days.While the pigs were in lairage they were given water but were not fed. After slaughter the caecal contents of all pigs were cultured to detect Salmonella spp.The organism was isolated from 70 % of 145 pigs killed after 1 day in lairage, 49 % of 143 pigs that had been in lairage for 2 days and 41 % of 135 pigs that had been held for 3 days.

“…Where large inocula, such as 80 g, are to be introduced into an enrichment medium as in an earlier paper on salmonellas in pig faeces, the R25 broth might not be so efficient as in this study. Both selenite F and Muller-Kauffmann tetrathionate can accept large inocula and might be more suitable media (Harvey, Price & Morgan, 1977).…”

Section: Discussionmentioning

confidence: 99%

A comparison of two modifications of Rappaport's enrichment medium (R25 and RV) for the isolation of salmonellas from sewage polluted natural water

Harvey

Price

1983

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The development of Rappaport's enrichment medium and elevated temperature incubation as methods of salmonella isolation is traced. The recent recording of a merger of the two techniques by means of Rappaport--Vassiliadis medium is noted (RV medium). In Cardiff, we have found an earlier modification of Rappaport's enrichment (R25) by Vassiliadis to be efficient in salmonella recovery from environment samples. The current study compares the two media using sewage polluted natural water as test material. Under the conditions of experiment, R25 was more successful in salmonella isolation than RV, although the later medium inhibited competitive organisms better. R25 is a convenient enrichment broth for routine use. In combination with pre-enrichment it allows the use of a loop for subculture rather than a pipette. This increases safe manipulation. It also produces a high proportion of positive isolations at the 24 h subculture time in contrast to other enrichment broths. For these reasons it forms an integral part of salmonella isolation methodology in our laboratory.