Salternamides A–D from a Halophilic <i>Streptomyces</i> sp. Actinobacterium

Kim, Seong-Hwan; Shin, Yeong Gil; Lee, So-Hyoung; Oh, Ki‐Bong; Lee, Sang Kook; Shin, Jongheon; Oh, Dong‐Chan

doi:10.1021/acs.jnatprod.5b00002

Cited by 42 publications

(31 citation statements)

References 20 publications

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“…RI18 and this compound inhibited influenza virus growth in plaque assays. A bioactive compound, xiamycin D isolated from Streptomyces sp (#HK18) culture inhabiting the topsoil in a Korean solar saltern was tested for antiviral activity against porcine epidemic diarrhea virus (PEDV) and results showed that xiamycin D had the strongest inhibitory effect on PEDV replication (13). Methylelaiophylin, an antiviral compound isolated from Streptomyces melanosporofaciens showed antiviral activity against Newcastle disease virus (NDV) (14).…”

Section: Introductionmentioning

confidence: 99%

Antiviral viral compound fromStreptomyces ghanaensislike strain against white spot syndrome virus (WSSV) of shrimp

Rajkumar¹,

Manimaran

Taju³

et al. 2018

Preprint

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35Actinomycetes isolates collected from different environments were screened for 36 antiviral activity against WSSV. One isolate designated as CAHSH-2 showed antiviral 37 activity against WSSV at the concentration of 0.2 mg per shrimp. The laboratory trial of 38 determining antiviral activity of ethyl acetate extract (EtOAcE) of CAHSH-2 against WSSV 39 was carried out 21 times since 2014. CAHSH-2 isolate which showed antiviral activity was 40 characterized and identified as Streptomyces ghanaensis like strain. Among the five fractions 41 obtained from EtOAcE of potential actinomycetes isolate, F1 was found to have strong 42 antiviral activity. The F1A and F1B sub-fractions from F1 fraction were subjected to GC-MS, 43 FTIR, 1 H and 13 C NMR analyses and, the compounds identified were di-n-octyl phthalate and 44 bis (2-methylheptyl) phthalate, respectively. Among these compounds, di-n-octyl phthalate 45 showed strong antiviral activity against WSSV. Molecular docking studies revealed that di-n-46 octyl phthalate was found to have high binding affinity with VP26 and VP28 proteins of 47 WSSV, whereas the bis (2-methylheptyl) phthalate showed low binding affinity with VP26 48 and VP28. The antiviral activity of EtOAcE of actinomycetes against WSSV was confirmed 49 by PCR, RT-PCR, Western blot and ELISA. The EA extract of active isolate was found to be 50 non-toxic to Artemia, post-larvae and adult Litopenaeus vannamei. 51 Importance 52 53White spot syndrome virus (WSSV) is an important shrimp viral pathogen and 54 responsible for huge economic loss to shrimp culture industry worldwide including India. The 55 global loss due to WSSV has been estimated about USD 10 billion and the loss continues at 56 the same extent even now. Various strategies have been followed to prevent or control 57 diseases of aquatic animals. In spite of various preventive and control strategies, WSSV has 58 been still persisting for more than two decades. No control strategies have so far been evolved 59 to put a break to WSSV. In this situation, an attempt was made in the present work to screen 60 some actinomycetes isolates for antiviral activity against WSSV. Among these isolates, one 61 isolate identified as Streptomyces ghanaensis like isolate CAHSH-2 showed activity against 62 WSSV. This article gives the information about the antiviral compound against WSSV and 63 the mechanism of viral inhibition. 64 65 66

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Section: Introductionmentioning

confidence: 99%

Antiviral viral compound fromStreptomyces ghanaensislike strain against white spot syndrome virus (WSSV) of shrimp

Rajkumar¹,

Manimaran

Taju³

et al. 2018

Preprint

View full text Add to dashboard Cite

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“…Salterns are the most extreme habitats in terms of salinity and they can harbor truly halophilic actinomycetes. However, the chemistry of actinobacteria in these hypersaline environments had been mostly neglected until the report of Kim et al [30], on the production of saltern amides with the ability to induce apoptosis in human cancer cells, as the first secondary metabolites from a saltern-derived Streptomyces sp. So far, several bioactive compounds such as xiamycins C-E from a halophilic Streptomyces sp., with potent antiviral activity against porcine epidemic diarrhea virus (PEDV) [12] and borrelidins C-E from a saltern-derived halophilic Nocardiopsis sp.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of Antibacterial Activity of Paludifilum halophilum and Identification of N-(1-Carboxy-ethyl)-phthalamic Acid as the Main Bioactive Compound

Frikha-Dammak

Fakhfakh

Belhaj

et al. 2020

BioMed Research International

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The aim of this study was to determine the combined effect of fermentation parameters and enhance the production of cellular biomass and antibacterial compounds from Paludifilum halophilum SMBg3 using the response surface methodology (RSM). Eight variables were screened to assess the effects of fermentation parameters on growth and metabolite production by Taguchi experimental design. Among these, the initial pH, temperature, and the percentage of MgSO4·7H2O in the medium were found to be most influential. The Box–Behnken design was applied to derive a statistical model for the optimization of these three fermentation parameters. The optimal parameters were initial pH: 8.3, temperature growth: 44°C, and MgSO4·7H2O: 1.6%, respectively. The maximum yield of biomass and metabolite production were, respectively, 11 mg/mL dry weight and 15.5 mm inhibition zone diameter against Salmonella enterica, which were in agreement with predicted values. The bioactive compounds were separated by the thick-layer chromatography technique and analyzed by GC/MS, NMR (1D and 2D), and Fourier-transform infrared spectroscopy (FT-IR). In addition to several fatty acids, N-(1-carboxy-ethyl)-phthalamic acid was identified as the main antibacterial compound. This element exhibited a potent activity against the ciprofloxacin-resistant Salmonella enterica CIP 8039 and Pseudomonas aeruginosa ATCC 9027 with a minimum inhibitory concentration (MIC) value range of 12.5–25 μg/mL. Results demonstrated that P. halophilum strain SMBg3 is a promising resource for novel antibacterial production due to its high-level yield potential and the capacity for large-scale fermentation.

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“…Novel anticancer molecules, Salternamide A-D, were isolated from a halophilic Streptomyces sp. isolated from a saltern on Shinui Island, in the Republic of Korea, and exhibited an extensive viability reduction in several cancer cell lines [111]. Among these molecules, Salternamide A inhibited the hypoxia-induced accumulation of HIF-1α in several cancer cell lines and suppressed the HIF-1α by downregulation of its upstream signaling pathways such as PI3K/Akt/mTOR, p42/p44 MAPK, and STAT3.…”

Section: Bacteriamentioning

confidence: 99%

Halophiles and Their Biomolecules: Recent Advances and Future Applications in Biomedicine

2019

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The organisms thriving under extreme conditions better than any other organism living on Earth, fascinate by their hostile growing parameters, physiological features, and their production of valuable bioactive metabolites. This is the case of microorganisms (bacteria, archaea, and fungi) that grow optimally at high salinities and are able to produce biomolecules of pharmaceutical interest for therapeutic applications. As along as the microbiota is being approached by massive sequencing, novel insights are revealing the environmental conditions on which the compounds are produced in the microbial community without more stress than sharing the same substratum with their peers, the salt. In this review are reported the molecules described and produced by halophilic microorganisms with a spectrum of action in vitro: antimicrobial and anticancer. The action mechanisms of these molecules, the urgent need to introduce alternative lead compounds and the current aspects on the exploitation and its limitations are discussed.

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Salternamides A–D from a Halophilic Streptomyces sp. Actinobacterium

Cited by 42 publications

References 20 publications

Antiviral viral compound fromStreptomyces ghanaensislike strain against white spot syndrome virus (WSSV) of shrimp

Antiviral viral compound fromStreptomyces ghanaensislike strain against white spot syndrome virus (WSSV) of shrimp

Enhancement of Antibacterial Activity of Paludifilum halophilum and Identification of N-(1-Carboxy-ethyl)-phthalamic Acid as the Main Bioactive Compound

Halophiles and Their Biomolecules: Recent Advances and Future Applications in Biomedicine

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