Sambiloto (Andrographis paniculata) Extract Improves the Performance of Animal Model Infected with Escherichia coli

Hidanah, Sri; Sabdoningrum, Emy Koestanti; Arif, Mohammad Anam Al; Ansori, Arif Nur Muhammad; Hasanah, Tanika Putri; Widaya, Laurensia Viola Artha

doi:10.37506/ijfmt.v14i4.12167

Cited by 5 publications

(1 citation statement)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The antibiotic disc was dipped into a test tube containing diluted antibiotics, sambiloto extract 5%, 10%, 20% and 40%. The selection of dilution is based on research conducted by Hidanah et al (2020) on Avian Pathogenic Eschericia coli and the addition one higher dose because Salmonella Pullorum is more pathogenic than Avian Pathogenic Eschericia coli. The sambiloto extracts then left to stand for 15 minutes, then removed using tweezers and placed on the surface of the media, the disc was pressed with tweezers with minimum distance of 15 mm between the discs and the discs that had been affixed on the surface of the media must not be moved or shifted.…”

Section: Sensitivity Test (Disk Diffusion Method)mentioning

confidence: 99%

Potential of Sambiloto (Andrographis paniculata Nees) Extract against Salmonella Pullorum

Hidanah

Sabdoningrum

Soeharsono

et al. 2022

IJAR

View full text Add to dashboard Cite

Background: Salmonella Pullorum are pathogenic bacteria that causes salmonellosis and causes heavy economic losses in the poultry industry and are zoonotic. Treatment of diseases caused by bacteria generally use antibiotics, but excessive administration of antibiotics causes bacterial resistance and residues in livestock. Major chemical constituents of Sambiloto are andrographolide and flavonoids. Andrographolide has antibacterial effect in addition to being antitoxic, anticancer, anti-inflammatory and antiallergic. Methods: The research was conducted by isolating and identifying Salmonella Pullorum on SSA media and a series of biochemical tests (TSIA, SIM, SCA, urea media and sugar test), manufacturing sambiloto extract, testing the sensitivity of several antibiotics using the disk diffusion method and testing the activation of sambiloto extract against Salmonella Pullorum using the disk diffusion and dilution methods. Result: The result show that sambiloto had antibacterial activity because it contained andrographolide, flavonoids, saponins, alkaloids and tannins and the lowest extract dose that effectively killed Salmonella Pullorum is concentrations of 20%.

show abstract

Section: Sensitivity Test (Disk Diffusion Method)mentioning

confidence: 99%

Potential of Sambiloto (Andrographis paniculata Nees) Extract against Salmonella Pullorum

Hidanah

Sabdoningrum

Soeharsono

et al. 2022

IJAR

View full text Add to dashboard Cite

show abstract

The activity of Meniran (Phyllanthus niruri Linn.) extract on Salmonella pullorum infected broilers

et al. 2022

View full text Add to dashboard Cite

Background and Aim: Pullorum is an acute and chronic disease caused by Salmonella pullorum, often infecting chicken farms. Pullorum disease treatment using antibiotics that do not follow the control dose can cause bacteria to become antibiotic-resistant. Meniran contributes to inhibiting and antagonizing bacteria and can increase the efficiency of chicken feed because of its bioactive compounds, including alkaloids, flavonoids, tannins, and saponins. This study aimed to determine the activity of Meniran extract (Phyllanthus niruri Linn.) in broilers infected with S. pullorum. Materials and Methods: In vitro study that was conducted includes phytochemical test, diffusion, and dilution methods using Meniran extract at 5%, 10%, 20%, and 40% concentrations and tylosin at 2% concentration. The data of the dilution method (minimum inhibitory concentration [MIC] and minimum bactericidal concentration [MBC]) were processed using probit analysis to determine LC50. In vivo study was conducted by randomly dividing 20 broilers into five treatment groups, four per group. The chickens (except in group P0–) were infected with S. pullorum aged 14 days. Then, the treatment was conducted according to the divided groups when the chickens were aged 21-34 days. The said treatments are P0– (uninfected S. pullorum and unadministered with Meniran extract), P0+ (infected with S. pullorum and unadministered with Meniran extract), and P1, P2, and P3 (infected with S. pullorum and administered with Meniran extract with 5%, 10%, and 20% concentrations, respectively). Data from the phytochemical test were analyzed as descriptive. The data from the diffusion method were analyzed using one-way analysis of variance (ANOVA) and Duncan's test. Then, the results of broilers' performance were analyzed using ANOVA and Duncan's test. Results: The phytochemical test showed positive for alkaloid, tannin, saponin, flavonoid, and steroid/triterpenoid. The diffusion method formed the largest zone at 40% concentration with 15.6 mm, while 20%, 10%, and 5% had average of 13.15 mm, 8.38 mm, and 5.8 mm, respectively. The dilution method (MIC and MBC) exhibited the antibacterial ability of Meniran extract against S. pullorum at 20% dose and LC50 14.118% concentration. The Meniran extract administration in broilers exhibited improved performance of chickens infected with S. pullorum, with the administration of 20% dose of Meniran extract showing the best result. Conclusion: About 20% concentration Meniran extract can serve as an antibacterial agent and showed the best results in broilers infected with S. pullorum.

show abstract

Polyphenols and Organic Acids as Alternatives to Antimicrobials in Poultry Rearing: A Review

et al. 2021

View full text Add to dashboard Cite

For decades antibiotics have been used in poultry rearing to support high levels of production. Nevertheless, several problems have arisen because of the misuse of antibiotics (i.e., antibiotic resistance, residues in animal products, environmental pollution). Thus, the European Union (EU) as well as the European Food Safety Authority (EFSA) promote action plans to diminish the use of antibiotics in animal production. Alternatives to antibiotics have been studied. Polyphenols (PPs) or organic acids (OAs) seem to be two accredited solutions. Phenolic compounds, such as phenols, flavonoids, and tannins exert their antimicrobial effect with specific mechanisms. In contrast, short chain fatty acids (SCFAs) and medium chain fatty acids (MCFAs), the OAs mainly used as antibiotics alternative, act on the pathogens depending on the pKa value. This review aims to collect the literature reporting the effects of these substances applied as antimicrobial molecules or growth promoter in poultry feeding (both for broilers and laying hens). Organic acids and PPs can be used individually or in blends, exploiting the properties of each component. Collected data highlighted that further research needs to focus on OAs in laying hens’ feeding and also determine the right combination in blends with PPs.

show abstract

Sambiloto (Andrographis paniculata) Extract Improves the Performance of Animal Model Infected with Escherichia coli

Cited by 5 publications

References 10 publications

Potential of Sambiloto (Andrographis paniculata Nees) Extract against Salmonella Pullorum

Potential of Sambiloto (Andrographis paniculata Nees) Extract against Salmonella Pullorum

The activity of Meniran (Phyllanthus niruri Linn.) extract on Salmonella pullorum infected broilers

Polyphenols and Organic Acids as Alternatives to Antimicrobials in Poultry Rearing: A Review

Contact Info

Product

Resources

About