Sample Geometry Influences the Outcome of in vitro Cytotoxicity Tests

Löbler, Marian; Behrend, D.; Maletz, R.; Schmitz, K.-P.

doi:10.1002/adem.200800106

Cited by 9 publications

(11 citation statements)

References 9 publications

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“…To calculate the relative viability, the cell viability detected on the polystyrene surface was set to 100%. The subsequent procedure was carried out as described previously 47…”

Section: Methodsmentioning

confidence: 99%

Surface functionalization of poly(ε‐caprolactone) improves its biocompatibility as scaffold material for bioartificial vessel prostheses

et al. 2011

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Within this study, chemically modified polymer surfaces were to be developed, which should enhance the subsequent immobilization of various bioactive substances. To improve the hemocompatibility and endothelialization of poly(ε-caprolactone) (PCL) intended as scaffold material for bioartificial vessel prostheses, terminal amino groups via ammonia (NH₃) plasma, oxygen (O₂) plasma/aminopropyltriethoxysilane (APTES), and 4,4'-methylenebis(phenyl isocyanate) (MDI)/water were provided. Then, immobilization of the anti-inflammatory and antithrombogenic model drug acetylsalicylic acid (ASA) and vascular endothelial growth factor (VEGF) were performed by employing N,N-disuccinimidyl carbonate (DSC) as crosslinker. Contact angle and fluorescence measurements, X-ray photoelectron spectroscopy and infrared spectroscopy confirmed the surface modification. Here the highest functionalization was observed for the O₂ plasma/APTES modification. Furthermore, biocompatibility studies demonstrated that the surface reactions have no negative influence, neither on the viability of L929 mouse fibroblasts, nor on primary or secondary hemostasis. Release studies showed that the immobilization of ASA and VEGF on the modified PCL surface via DSC is greatly improved compared to the adsorption-only reference. The advantage of DSC is that it immobilizes both bioactive substances via non-hydrolyzable and/or hydrolyzable covalent bonding. The highest ASA loading and cumulative release was detected using NH₃ plasma-activated PCL samples. For VEGF, the O₂ plasma/APTES-modified PCL samples were most efficient with regard to loading and cumulative release. In conclusion, both modifications are promising methods to optimize PCL as scaffold material for bioartificial vessel prostheses.

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“…To calculate the relative viability, the cell viability detected on the polystyrene surface was set to 100%. The subsequent procedure was carried out as described previously 47…”

Section: Methodsmentioning

confidence: 99%

Surface functionalization of poly(ε‐caprolactone) improves its biocompatibility as scaffold material for bioartificial vessel prostheses

et al. 2011

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“…Die Silberionenfreisetzung ist dabei stark beeinflusst vom Nanokompositaufbau (Wasseraufnahme der Kunststoffmatrix, Nanopartikelgröße und -verteilung) [25], von der verwendeten Probengeometrie [26] und den Eigenschaften des umgebenden Mediums (Temperatur, pH-Wert, Ionen-und Proteinkonzentration) [27]. Die Silberionenfreisetzung ist dabei stark beeinflusst vom Nanokompositaufbau (Wasseraufnahme der Kunststoffmatrix, Nanopartikelgröße und -verteilung) [25], von der verwendeten Probengeometrie [26] und den Eigenschaften des umgebenden Mediums (Temperatur, pH-Wert, Ionen-und Proteinkonzentration) [27].…”

Section: Angewandte Methoden Und Materialienunclassified

“…Hierbei werden unter Verwendung von kurzen und ultrakurzen Laserpulsen feste Werkstoffe [14,15] oder Mikropartikel [16] Die antibakterielle Wirksamkeit von polymeren Nanokompositen mit eingebetteten Silbernanopartikeln basiert auf der Freisetzung von Silberionen in das umgebende Medium. Die Silberionenfreisetzung ist dabei stark beeinflusst vom Nanokompositaufbau (Wasseraufnahme der Kunststoffmatrix, Nanopartikelgröße und -verteilung) [25], von der verwendeten Probengeometrie [26] und den Eigenschaften des umgebenden Mediums (Temperatur, pH-Wert, Ionen-und Proteinkonzentration) [27]. Zur Analyse der Silberionenfreisetzung aus lasergenerierten TPU-Nanokompositen wurden Proben ( [17].…”

Section: Problemstellungunclassified

Laser‐Based Generation of Nanocomposites without Matrix‐Coupling Agents for Bioactive Medical Devices

Schwenke

Wagener

Weiß

et al. 2013

Chemie Ingenieur Technik

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Zur Ausschöpfung des Potenzials funktionaler Nanomaterialen bedarf es neuartiger Produktionsverfahren, die die Dispersion der Nanopartikel entlang der Prozesskette sicherstellen. Am Beispiel von Medizinprodukten mit antibakteriellem Schutz vor Biofilmbildung auf Basis von Silbernanopartikeln werden die Volumenintegration in Kunststoffe und die Nanopartikelsynthese durch Laserabtrag in Flüssigkeiten vorgestellt. Die antibakterielle Wirksamkeit der Nanokomposite und Verarbeitbarkeit zu Prototypen werden gezeigt.New production technologies are required to benefit of the full potential of nanocomposites by homogeneous dispersion of nanoparticles along the process chain. Synthesis of silver nanoparticles by laser ablation in liquid and their integration into polymers are presented. Antibacterial properties of these materials and processability into prototypes for medical devices with antibacterial protection are demonstrated.

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“…About 3000 cells were seeded directly on to each test specimen and grown at 37°C, 5% CO 2 and 95% relative humidity. Initial cell density allowed cultivation for two additional days without reaching cell saturation 23. After 2 days the cell culture medium was replaced by 200 μL of freshly prepared 10% CellQuanti‐Blue reagent (BioAssay Systems, Hayward, CA, purchased through Biotrend Chemikalien GmbH, Köln, Germany) in cell culture medium and incubated for another 2 h. Cellular reductase activity was quantified by fluorescence measurements (Fluostar Optima, BMG, Offenburg, Germany; excitation wavelength 544 nm, emission wavelength 590 nm).…”

Section: Methodsmentioning

confidence: 99%

Polymers and drugs suitable for the development of a drug delivery drainage system in glaucoma surgery

et al. 2011

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Implantation of a glaucoma drainage system is an appropriate therapeutic intervention in some glaucoma patients. However, one drawback with this approach is the fibrotic tissue response to the implant material, leading to reduced flow of aqueous liquid or complete blockage of the drainage system. As a basis for developing an aqueous shunt we report here investigations with poly(3-hydroxybutyrate) (P(3HB)) and poly(4-hydroxybutyrate) (P(4HB)) as polymer matrices and with paclitaxel (PTX) and triamcinolone acetonide (TA) as drugs that might, in combination, delay or prevent the process of fibrosis by reducing fibroblast activity. P(3HB) and P(4HB) were fabricated into test prototypes with 500 μm outer and 200 μm inner diameter and ∼1 cm length. The antiproliferative agent PTX and the anti-inflammatory agent TA were incorporated into the polymer matrices and were released by diffusion. In vitro cell assays demonstrated that the polymers have the potential to reduce fibroblast viability, while TA showed differential inhibition of Tenon fibroblasts, but not cornea keratocytes. Implantation of polymer disks and prototype devices into rabbit eyes confirmed the good biocompatibility of the materials. The combined use of a poly(hydroxybutyrate) polymer with PTX or TA has the potential to reduce the fibrosis associated with conventional glaucoma drainage systems.

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Sample Geometry Influences the Outcome of in vitro Cytotoxicity Tests

Cited by 9 publications

References 9 publications

Surface functionalization of poly(ε‐caprolactone) improves its biocompatibility as scaffold material for bioartificial vessel prostheses

Surface functionalization of poly(ε‐caprolactone) improves its biocompatibility as scaffold material for bioartificial vessel prostheses

Laser‐Based Generation of Nanocomposites without Matrix‐Coupling Agents for Bioactive Medical Devices

Polymers and drugs suitable for the development of a drug delivery drainage system in glaucoma surgery

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