Sample preparation and liquid chromatography–tandem mass spectrometry for the analysis of selected Pacific ciguatoxins in blood samples

Wang, Zhihong; Fuquay, Jennifer Maucher; Ledreux, Aurélie; Barbieri, Michelle; Ramsdell, John S.

doi:10.1016/j.chroma.2020.461050

Cited by 3 publications

(2 citation statements)

References 27 publications

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“…Sample preparation is an essential component of MS analysis of food and biological matrices. To date, there have been many clean-up techniques used in the sample preparation of urine, blood [33,34], and food matrices [35][36][37], including SPE, SPME, liquid-liquid extraction, and QuEChERS methods. As an easy-to-use sample preparation, QuECh-ERS is used to clean up the drugs in the sample in this study.…”

Section: Extraction Condition Optimizationmentioning

confidence: 99%

Rapid screening for 315 drugs in food and biological matrices by ultrahigh‐performance liquid chromatography quadrupole time of flight mass spectrometry and its application to a specific incident

Guo

Yang

Zhang

2022

Separation Science Plus

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Comprehensive screening of harmful chemical drugs in food is an essential analytical tool in the investigation of food poisoning. Meanwhile, determining harmful chemical drugs in biological matrices is a supplement to support the screening results in food. In this study, we developed a screening method for 315 harmful chemical drugs in food and biological matrices based on ultrahigh-performance liquid chromatography quadrupole time of flight mass spectrometry. The food and gastric juice sample was extracted by 1.0% formic acid acetonitrile and acetonitrile, respectively, and cleaned up by a C18 sorbent. Urine and serum were directly extracted by 1.0 % formic acid acetonitrile. Then the data was acquired and screened for drugs using a self-built library. The recovery, accuracy, and limit of quantitation for methodology were investigated. The method was validated by other three laboratories in different areas in China using different mass spectrometric techniques. For screening, the coefficient of variation of the retention times was below 5%. The recoveries for the drugs in nine matrices were 70-120%.At last, the developed method was applied to a real incident, demonstrating its suitability for the sensitive and fast screening of harmful chemical drugs in food and biological samples.

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Section: Extraction Condition Optimizationmentioning

confidence: 99%

Rapid screening for 315 drugs in food and biological matrices by ultrahigh‐performance liquid chromatography quadrupole time of flight mass spectrometry and its application to a specific incident

Guo

Yang

Zhang

2022

Separation Science Plus

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“…Therefore, these findings suggest the biotransformation of ciguatoxins into elasmobranch‐specific congeners and recommend reconsidering our understanding about the bioaccumulation pathways of CTX. Pacific ciguatoxins (P‐CTX‐1, P‐CTX‐2, P‐CTX‐3C) in human blood plasma was accessed by LC‐MS/MS analysis while following appropriate sample preparation strategies (Wang et al, 2020). The toxin separation was made possible by a C8 column other than the most common C18 reverse‐phase adopted by all the above investigators.…”

Section: Types Of Bio‐toxinmentioning

confidence: 99%

Recent advancements in LC‐MS based analysis of biotoxins: Present and future challenges

Panda

Dash

Manickam

et al. 2021

Mass Spectrometry Reviews

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There has been a rising concern regarding the harmful impact of biotoxins, source of origin, and the determination of the specific type of toxin. With numerous reports on their extensive spread, biotoxins pose a critical challenge to figure out their parent groups, metabolites, and concentration. In that aspect, liquid chromatography‐mass spectrometry (LC‐MS) based analysis paves the way for its accurate identification and quantification. The biotoxins are ideally categorized as phytotoxins, mycotoxins, shellfish‐toxins, ciguatoxins, cyanotoxins, and bacterial toxins such as tetrodotoxins. Considering the diverse nature of biotoxins, both low‐resolution mass spectrometry (LRMS) and high‐resolution mass spectrometry (HRMS) methods have been implemented for their detection. The sample preparation strategy for complex matrix usually includes “QuEChERS” extraction or solid‐phase extraction coupled with homogenization and centrifugation. For targeted analysis of biotoxins, the LRMS consisting of a tandem mass spectrometer operating in multiple reaction monitoring mode has been widely implemented. With the help of the reference standard, most of the toxins were accurately quantified. At the same time, the suspect screening and nontarget screening approach are facilitated by the HRMS platforms during the absence of reference standards. Significant progress has also been made in sampling device employment, utilizing novel sample preparation strategies, synthesizing toxin standards, employing hybrid MS platforms, and the associated data interpretation. This critical review attempts to elucidate the progress in LC‐MS based analysis in the determination of biotoxins while pointing out major challenges and suggestions for future development.

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Ciguatera Mini Review: 21st Century Environmental Challenges and the Interdisciplinary Research Efforts Rising to Meet Them

Loeffler

Tartaglione

Friedemann

et al. 2021

IJERPH

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Globally, the livelihoods of over a billion people are affected by changes to marine ecosystems, both structurally and systematically. Resources and ecosystem services, provided by the marine environment, contribute nutrition, income, and health benefits for communities. One threat to these securities is ciguatera poisoning; worldwide, the most commonly reported non-bacterial seafood-related illness. Ciguatera is caused by the consumption of (primarily) finfish contaminated with ciguatoxins, potent neurotoxins produced by benthic single-cell microalgae. When consumed, ciguatoxins are biotransformed and can bioaccumulate throughout the food-web via complex pathways. Ciguatera-derived food insecurity is particularly extreme for small island-nations, where fear of intoxication can lead to fishing restrictions by region, species, or size. Exacerbating these complexities are anthropogenic or natural changes occurring in global marine habitats, e.g., climate change, greenhouse-gas induced physical oceanic changes, overfishing, invasive species, and even the international seafood trade. Here we provide an overview of the challenges and opportunities of the 21st century regarding the many facets of ciguatera, including the complex nature of this illness, the biological/environmental factors affecting the causative organisms, their toxins, vectors, detection methods, human-health oriented responses, and ultimately an outlook towards the future. Ciguatera research efforts face many social and environmental challenges this century. However, several future-oriented goals are within reach, including digital solutions for seafood supply chains, identifying novel compounds and methods with the potential for advanced diagnostics, treatments, and prediction capabilities. The advances described herein provide confidence that the tools are now available to answer many of the remaining questions surrounding ciguatera and therefore protection measures can become more accurate and routine.

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Sample preparation and liquid chromatography–tandem mass spectrometry for the analysis of selected Pacific ciguatoxins in blood samples

Cited by 3 publications

References 27 publications

Rapid screening for 315 drugs in food and biological matrices by ultrahigh‐performance liquid chromatography quadrupole time of flight mass spectrometry and its application to a specific incident

Rapid screening for 315 drugs in food and biological matrices by ultrahigh‐performance liquid chromatography quadrupole time of flight mass spectrometry and its application to a specific incident

Recent advancements in LC‐MS based analysis of biotoxins: Present and future challenges

Ciguatera Mini Review: 21st Century Environmental Challenges and the Interdisciplinary Research Efforts Rising to Meet Them

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