Sample preparation optimization of insects and zebrafish for whole-body mass spectrometry imaging

Ma, Lianlian; Xie, Qingrong; Du, Mingyi; Huang, Yudi; Chen, Yingying; Chen, Dong; Xu, Yijuan; Han-hong, XU; Wu, Xinzhou; Yin, Zhibin

doi:10.1007/s00216-022-04102-7

Cited by 10 publications

(12 citation statements)

References 63 publications

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“…Among them, the MS image of PE(36:1p) showed that it was distributed in the brain of S. invicta , whereas PE(38:5) mainly accumulated in the brain with a slight distribution in part of the abdomen. Additionally, PC(36:3) was located in part of the abdomen, whereas most TG species, such as TG(51:2) and TG(52:2), were found to accumulate throughout the whole‐body section of S. invicta , which is in agreement with previous reports 45,51 …”

Section: Resultssupporting

confidence: 92%

“…To ensure the accuracy of the experiment, all S. invicta used for MSI analysis were processed in the same batch as those for metabolomics analysis. Briefly, S. invicta was snap‐frozen in liquid nitrogen and then uniformly embedded in 10% (w/v) gelatin solution 45 . The cross‐sections of red fire ants were sectioned at −20 °C using a freezing microtome (CM1950; Leica Microsystem Inc., Wetzlar, Germany) with a thickness of 30 μm and mounted onto indium tin oxide (ITO)‐coated glass slides.…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, S. invicta was snap-frozen in liquid nitrogen and then uniformly embedded in 10% (w/v) gelatin solution. 45 The cross-sections of red fire ants were sectioned at −20 °C using a freezing microtome (CM1950; Leica Microsystem Inc., Wetzlar, Germany) with a thickness of 30 μm and mounted onto indium tin oxide (ITO)-coated glass slides. The slides were dried in a desiccator under vacuum for 30 min at room temperature.…”

Section: Sample Preparation Of S Invicta For Aunp-assisted Ldi-msi An...mentioning

confidence: 99%

“…Additionally, PC(36:3) was located in part of the abdomen, whereas most TG species, such as TG(51:2) and TG(52:2), were found to accumulate throughout the whole-body section of S. invicta, which is in agreement with previous reports. 45,51 3.5 Metabolic pathway analysis of S. invicta in response to indoxacarb The aforementioned metabolic results indicate that indoxacarb treatment could induce significant cross-regulation of metabolites. Thus, we determined the metabolism pathways of S. invicta in response to indoxacarb, including pentose and glucuronate interconversions, starch and sucrose metabolism, alanine, aspartate, and glutamate metabolism, and pyrimidine metabolism (Fig.…”

Section: Spatial Distribution Of Differential Metabolites Of Indoxaca...mentioning

confidence: 99%

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Integrated mass spectrometry imaging and metabolomics reveals sublethal effects of indoxacarb on the red fire ant Solenopsis invicta

Yin

et al. 2023

Pest Management Science

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BACKGROUND Indoxacarb, representing an efficient insecticide, is normally made into a bait to spread the poison among red fire ants so that it can be widely applied in the prevention and control of Solenopsis invicta. However, the potential toxicity mechanism of S. invicta in response to indoxacarb remains to be explored. In this study, we integrated mass spectrometry imaging (MSI) and untargeted metabolomics methods to reveal disturbed metabolic expression levels and spatial distribution within the whole‐body tissue of S. invicta treated with indoxacarb. RESULTS Metabolomics results showed a significantly altered level of metabolites after indoxacarb treatment, such as carbohydrates, amino acids and pyrimidine and derivatives. Additionally, the spatial distribution and regulation of several crucial metabolites resulting from the metabolic pathway and lipids can be visualized using label‐free MSI methods. Specifically, xylitol, aspartate, and uracil were distributed throughout the whole body of S. invicta, while sucrose‐6′‐phosphate and glycerol were mainly distributed in the abdomen of S. invicta, and thymine was distributed in the head and chest of S. invicta. Taken together, the integrated MSI and metabolomics results indicated that the toxicity mechanism of indoxacarb in S. invicta is closely associated with the disturbance in several key metabolic pathways, such as pyrimidine metabolism, aspartate metabolism, pentose and glucuronate interconversions, and inhibited energy synthesis. CONCLUSION Collectively, these findings provide a new perspective for the understanding of toxicity assessment between targeted organisms S. invicta and pesticides. © 2023 Society of Chemical Industry.

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Section: Resultssupporting

confidence: 92%

Section: Methodsmentioning

confidence: 99%

Section: Sample Preparation Of S Invicta For Aunp-assisted Ldi-msi An...mentioning

confidence: 99%

Section: Spatial Distribution Of Differential Metabolites Of Indoxaca...mentioning

confidence: 99%

See 2 more Smart Citations

Integrated mass spectrometry imaging and metabolomics reveals sublethal effects of indoxacarb on the red fire ant Solenopsis invicta

Yin

et al. 2023

Pest Management Science

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“…The tissue sections are then mounted onto the glass slides, metal targets or indium-tin oxide (ITO) coated glass slides. Before sectioning fresh frozen tissues, various embedding media can be used to preserve the morphology of the tissues and assist with the tissue section, including optimal cutting temperature medium (OCT), carboxymethyl cellulose, gelatin, agarose or ice ( 121 , 187 – 191 ). However, OCT could suppress analyte signals and is not recommended in MSI ( 189 , 192 ).…”

Section: Mass Spectrometry Imaging In Proteomics Lipidomics and Metab...mentioning

confidence: 99%

Applications of spatially resolved omics in the field of endocrine tumors

et al. 2023

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Endocrine tumors derive from endocrine cells with high heterogeneity in function, structure and embryology, and are characteristic of a marked diversity and tissue heterogeneity. There are still challenges in analyzing the molecular alternations within the heterogeneous microenvironment for endocrine tumors. Recently, several proteomic, lipidomic and metabolomic platforms have been applied to the analysis of endocrine tumors to explore the cellular and molecular mechanisms of tumor genesis, progression and metastasis. In this review, we provide a comprehensive overview of spatially resolved proteomics, lipidomics and metabolomics guided by mass spectrometry imaging and spatially resolved microproteomics directed by microextraction and tandem mass spectrometry. In this regard, we will discuss different mass spectrometry imaging techniques, including secondary ion mass spectrometry, matrix-assisted laser desorption/ionization and desorption electrospray ionization. Additionally, we will highlight microextraction approaches such as laser capture microdissection and liquid microjunction extraction. With these methods, proteins can be extracted precisely from specific regions of the endocrine tumor. Finally, we compare applications of proteomic, lipidomic and metabolomic platforms in the field of endocrine tumors and outline their potentials in elucidating cellular and molecular processes involved in endocrine tumors.

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Metabolomics and mass spectrometry imaging reveal the chronic toxicity of indoxacarb to adult zebrafish (Danio rerio) livers

Yin

Xie

et al. 2023

Journal of Hazardous Materials

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Sample preparation optimization of insects and zebrafish for whole-body mass spectrometry imaging

Cited by 10 publications

References 63 publications

Integrated mass spectrometry imaging and metabolomics reveals sublethal effects of indoxacarb on the red fire ant Solenopsis invicta

Integrated mass spectrometry imaging and metabolomics reveals sublethal effects of indoxacarb on the red fire ant Solenopsis invicta

Applications of spatially resolved omics in the field of endocrine tumors

Metabolomics and mass spectrometry imaging reveal the chronic toxicity of indoxacarb to adult zebrafish (Danio rerio) livers

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