Sample stacking revisited: A personal perspective

Chien, Ring‐Ling

doi:10.1002/elps.200390057

Cited by 131 publications

(100 citation statements)

References 102 publications

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“…Nevertheless, when the sample is injected electrokinetically (applying a voltage for a certain time), the analytes are introduced into the capillary depending on the EOF of the sample, and of the charge and mobility of the analytes. If the preconcentration of the analytes is made during the electrokinetic injection, then the preconcentration is performed by field-amplified sample stacking (FASS), also called field-enhanced sample injection (FESI), or field-amplified sample injection (FASI) [91][92][93].…”

Section: On-column Preconcentration Techniques In Sensitive Chiral Anmentioning

confidence: 99%

Sensitive chiral analysis by capillary electrophoresis

García-Ruiz

Marina

2006

Electrophoresis

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Sensitive chiral analysis by capillary electrophoresisIn this review, an updated view of the different strategies used up to now to enhance the sensitivity of detection in chiral analysis by CE will be provided to the readers. With this aim, it will include a brief description of the fundamentals and most of the recent applications performed in sensitive chiral analysis by CE using offline and online sample treatment techniques (SPE, liquid-liquid extraction, microdialysis, etc.), on-column preconcentration techniques based on electrophoretic principles (ITP, stacking, and sweeping), and alternative detection systems (spectroscopic, spectrometric, and electrochemical) to the widely used UV-Vis absorption detection.

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Section: On-column Preconcentration Techniques In Sensitive Chiral Anmentioning

confidence: 99%

Sensitive chiral analysis by capillary electrophoresis

García-Ruiz

Marina

2006

Electrophoresis

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“…The use of stacking techniques has made possible to improve, in some cases considerably, the sensitivity in CE [60,61]. These stacking techniques have also been used in the chiral separation of triadimenol [23] and phenoxy acid herbicides [34].…”

Section: Chiral Analysis Of Pesticidesmentioning

confidence: 99%

Chiral analysis of pollutants and their metabolites by capillary electromigration methods

et al. 2005

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Chiral analysis of pollutants and their metabolites by capillary electromigration methodsChiral separation of enantiomers is one of the most challenging tasks for any analytical technique including CE. Since the first report in 1985 showing the great possibilities of CE for the separation of chiral compounds, the amount of publications concerning this topic has quickly increased. Although chiral electromigration methods have mainly been used for enantioseparation of drugs and pharmaceuticals, they have also been applied to analyze chiral pollutants. This article intends to provide an updated overview, including works published till January 2005, on the principal applications of CE to the chiral analysis of pollutants and their metabolites, with especial emphasis on articles published in the last 10 years. The main advantages and drawbacks regarding the use of CE for chiral separation of pollutants are addressed including some discussion on the foreseen trends of electromigration procedures applied to chiral analysis of contaminants. IntroductionSeparation of chiral compounds is an interesting and challenging topic of research in many analytical chemistry areas, especially in the biomedical, pharmaceutical, and environmental fields where pure enantiomeric forms are widely required. It is already well-known that enantiomers, in spite of their very similar structure, when exposed to an identical biological environment can show very different biological activity. Furthermore, as a general rule, when the toxicological effect of an active substance is directly related to its desired biological activity, the most active isomer is usually the most toxic one Abbreviations: Allyl-TER, 1-allylterguride; ANDSA, 7-aminonaphthalene-1,3-disulfonic acid; ANSA, 5-aminonaphthalene-1-sulfonic acid; ANTS, 8-aminonaphthalene-1,3,6-trisulfonic acid; CA, chrysanthemic acid; CM-ª-CD, carboxymethylated-g-CD; CMBA, 2-(4-chlorophenyl)-3-methylbutanoic acid; 2,2-CPPA, 2-(2-chlorophenoxy)-propionic acid; 2,3-CPPA, 2-(3-chlorophenoxy)-propionic acid; 2,4-CPPA, 2-(4-chlorophenoxy)-propionic acid; â-CD-NH 2 , 6-monodeoxy-6-monoamino-b-CD; â-CD- (NH 3 )

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“…In dynamic pH junction, pH differences along the separation path are used to enhance the sensitivity. These methods have been well described in recent review articles [15][16][17][18]. When used individually, sensitivity enhancement factors (SEFs) ranging from few tens to few thousands can be achieved.…”

Section: Introductionmentioning

confidence: 99%

Integration of various stacking processes in carrier ampholyte-based capillary electrophoresis

Busnel

Saux

Descroix

et al. 2008

Journal of Chromatography A

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Field-enhanced sample stacking, field-enhanced sample injection as well as electrokinetic supercharging have been successfully integrated in carrier ampholyte-based capillary electrophoresis. Through the analysis of different test sample mixtures, it has been shown that the carrier ampholyte-based background electrolytes, in spite of their very low conductivity, allow efficient online preconcentration of analytes by fieldamplified techniques. Sensitivity enhancement factors of the same magnitude as those usually encountered with classical conductive background electrolytes have been obtained in such carrier ampholyte-based buffers. Depending on the online preconcentration method that has been integrated, sensitivity enhancement factors between 50 and several thousands have been reached.

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Sample stacking revisited: A personal perspective

Cited by 131 publications

References 102 publications

Sensitive chiral analysis by capillary electrophoresis

Sensitive chiral analysis by capillary electrophoresis

Chiral analysis of pollutants and their metabolites by capillary electromigration methods

Integration of various stacking processes in carrier ampholyte-based capillary electrophoresis

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