Samsoeum water extract attenuates allergic airway inflammation via modulation of Th1/Th2 cytokines and decrease of iNOS expression in asthmatic mice

Abstract

BackgroundSamsoeum has long been used in Korea and other Asian countries as a traditional medicine to treat various diseases. In the present study, we investigated the antiasthma effect of the herbal medicine Samsoeum water extract (SSEW) using an in vivo ovalbumin (OVA)-induced asthmatic model.MethodsFemale BALB/c mice were sensitized by an intraperitoneal injection of OVA and subsequently challenged with nebulized OVA. We investigated the number of inflammatory cells, the production of Th1/Th2 cytokines and chemokine in bronchoalveolar lavage fluid (BALF), histological changes in lung tissue, the infiltration of inflammatory cells and hyperplasia of goblet cells in lung tissue, the levels of immunoglobulin E (IgE) in BALF and plasma, and the expression of inducible nitric oxide synthase (iNOS) in lung tissue.ResultsOur findings indicated that SSEW decreased the accumulation of inflammatory cells (particularly, eosinophil and neutrophil) and regulated the balance in the production of Th1/Th2 cytokines and chemokine in BALF. Moreover, SSEW suppressed the level of IgE in BALF and plasma, and inhibited the infiltration of inflammatory cells, hyperplasia of goblet cells, and the expression of iNOS in lung tissue.ConclusionsCollectively, these results suggest that, because of its anti-inflammatory and antiasthma properties, SSEW may be useful in reducing airway inflammation in the treatment of allergic asthma.

Attenuation of antigen-specific T helper 1 immunity by Neolitsea hiiranensis and its derived terpenoids

“…Attenuation of IFN- γ production may provide a therapeutic strategy to management these immune disorders. Several natural compounds from medicinal plants have been shown to possess therapeutic potentials for Th1-associated diseases (Jeon et al, 2015; Wu et al, 2014). The extracts of mushroom Phellinus igniarius were demonstrated to relieve the symptoms of EAE through inhibition of IFN- γ production and lymphocyte proliferation (Li et al, 2014b).…”

“…To discover new botanicals with differential immunomodulatory effects on T-cell function may provide more therapeutics for different T-cell-mediated immune disorders. Many natural compounds isolated from medicinal plants have been shown to possess therapeutic potentials for Th1-associated diseases (Jeon et al, 2015; Wu et al, 2014). …”

Soshiho-tang water extract inhibits ovalbumin-induced airway inflammation via the regulation of heme oxygenase-1

“…To induce asthma, OVA sensitization and airway challenge were performed as described previously [ 14 , 15 ]. The mice were sensitized on days 0 and 14 by intraperitoneal injection of 20 μg OVA emulsified in 2 mg aluminum hydroxide in 200 μL phosphate buffered saline (PBS) buffer (pH 7.4).…”

LC–MS/MS analysis of puerarin and 18β‐glycyrrhetinic acid in human plasma after oral administration of Samso‐eum and its application to pharmacokinetic study

“…Several studies applying HPLC‐UV and LC–MS methods have reported simultaneous quantification of SSE (Seo, Kim, Huang, & Shin, ; Weon, Yang, Lee, Ma, & Ma, ). Previous pharmacological studies have shown that SSE possesses immune‐regulating, adipogenesis‐attenuating, anticancer, and anti‐inflammation properties (Jeon, Shin, Shin, & Lee, ; Jeong, Yoo, Seo, & Shin, ; Kim et al, ; Kim, Yim, & Ma, ; Ryu et al, ). To date, SSE studies have focused on the development of analytical methods to assess its pharmacological activity.…”