Sarco(endo)plasmic reticulum calcium pump isoforms in paralyzed rat slow muscle

Talmadge, Robert J.; Paalani, Michael

doi:10.1016/j.bbagen.2007.03.013

Cited by 9 publications

(17 citation statements)

References 28 publications

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“…In accordance with this, inactivation of Ras (Zádor and Wuytack 2003) or calcineurin (Zádor et al 2005) in the regenerating soleus induced a decline in the expression of MyHC1 but left the expression of SERCA2a unchanged. Although the nervous control of muscle gene expression may differ in the regenerating vs normal muscle (Moreno et al 2003), a number of studies have shown that the expression of the corresponding SERCA and MyHC isoforms are also dissociated in paralyzed cat and rat soleus Talmadge and Paalani 2007), in overloaded soleus (Awede et al 1999), and in denervated soleus muscles (Leberer et al 1986;Hämäläinen and Pette 2001;Roy et al 2002).…”

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confidence: 99%

The Effect of Passive Movement on Denervated Soleus Highlights a Differential Nerve Control on SERCA and MyHC Isoforms

Szabó

Wuytack

Zádor

2008

J Histochem Cytochem.

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S U M M A R Y The sarco-endoplasmic reticulum Ca 21 ATP-ase (SERCA) and myosin heavy chain (MyHC) levels were measured in hindlimb-denervated and selectively denervated rat soleus muscles. Selective denervation allowed passive movement of the soleus, whereas hindlimb denervation rendered it to passivity. To minimize chronic effects, we followed the changes only for 2 weeks. Selective denervation resulted in less muscle atrophy, a faster slow-to-fast transition of MyHC isoforms, and less coordinated expressions of the slow vs fast isoforms of MyHC and SERCA. Generally, expression of the slow-twitch type SERCA2a was found to be less dependent, whereas the slow-twitch type MyHC1 was the most dependent on innervation. Our study shows that passive movement is able to ameliorate denervationinduced atrophy of the soleus and that it also accentuates the dyscoordination in the expression of the corresponding slow and fast isoforms of MyHC and SERCA.

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confidence: 99%

The Effect of Passive Movement on Denervated Soleus Highlights a Differential Nerve Control on SERCA and MyHC Isoforms

Szabó

Wuytack

Zádor

2008

J Histochem Cytochem.

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“…SERCA density. We also examined the densities of both SERCA isoforms in the muscle following the disuse relative to the baseline because it has been reported that there is an extensive upregulation of SERCA1 in disused rat muscle (44,47) and in paralyzed human (46). It is possible that the increase in SERCA1 expression underlies the faster muscle relaxation in rat following hindlimb casting (13) or spinal cord transection (48).…”

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confidence: 99%

Effect of 23-day muscle disuse on sarcoplasmic reticulum Ca²⁺ properties and contractility in human type I and type II skeletal muscle fibers

Lamboley

Wyckelsma

Perry

et al. 2016

Journal of Applied Physiology

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Lamboley CR, Wyckelsma VL, Perry BD, McKenna MJ, Lamb GD. Effect of 23-day muscle disuse on sarcoplasmic reticulum Ca 2ϩ properties and contractility in human type I and type II skeletal muscle fibers. J Appl Physiol 121: 483-492, 2016. First published June 30, 2016 doi:10.1152/japplphysiol.00337.2016.-Inactivity negatively impacts on skeletal muscle function mainly through muscle atrophy. However, recent evidence suggests that the quality of individual muscle fibers is also altered. This study examined the effects of 23 days of unilateral lower limb suspension (ULLS) on specific force and sarcoplasmic reticulum (SR) Ca 2ϩ content in individual skinned muscle fibers. Muscle biopsies of the vastus lateralis were taken from six young healthy adults prior to and following ULLS. After disuse, the endogenous SR Ca 2ϩ content was ϳ8% lower in type I fibers and maximal SR Ca 2ϩ capacity was lower in both type I and type II fibers (Ϫ11 and Ϫ5%, respectively). The specific force, measured in single skinned fibers from three subjects, decreased significantly after ULLS in type II fibers (Ϫ23%) but not in type I fibers (Ϫ9%). Western blot analyses showed no significant change in the amounts of myosin heavy chain (MHC) I and MHC IIa following the disuse, whereas the amounts of sarco(endo)plasmic reticulum Ca 2ϩ -ATPase 1 (SERCA1) and calsequestrin increased by ϳ120 and ϳ20%, respectively, and the amount of troponin I decreased by ϳ21%. These findings suggest that the decline in force and power occurring with muscle disuse is likely to be exacerbated in part by reductions in maximum specific force in type II fibers, and in the amount of releasable SR Ca 2ϩ in both fiber types, the latter not being attributable to a reduced calsequestrin level. Furthermore, the ϳ3-wk disuse in human elicits change in SR properties, in particular a more than twofold upregulation in SERCA1 density, before any fiber-type shift. IT IS WELL KNOWN that chronic muscle disuse in young healthy adults, as induced by spaceflight, bedrest, or immobilization, adversely affects muscle function. Although the loss of skeletal muscle mass induced by the disuse is widely acknowledged to contribute to this impairment, it has become increasingly evident that contractile dysfunction is disproportionately greater than decrements in cross-sectional area (CSA), as indicated by a decrease of specific force, suggesting a significant role played by decreased muscle "quality" in the dysfunction (4,9,15,33,51). It has been recently reported that the reduction of muscle quality can be attributed to a complex interaction of many factors that can affect neuromuscular transmission (4,24,42), muscle architecture (8, 9), muscle fiber phenotype (14,38), and contractile apparatus properties (20,21).Studies examining human muscle biopsies following a longterm period of muscle disuse have reported an overall shift in myosin heavy chain (MHC) isoforms from I to IIa to IIx (51), though this is not seen with shorter term disuse (6, 30). Interestingly, measures of contractile functio...

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“…The lack of decline in SERCA2a expression (mRNA and protein) that we observed upon denervation is in agreement with the steady SERCA2a levels in spinal cord isolation , in spinal cord transsection (Talmadge and Paalani, 2007), in regeneratingdenervated muscles (Zádor and Wuytack, 2003;Zádor et al, 2005) and in overloaded soleus muscles (Awede et al, 1999).…”

Section: Denervation Modelssupporting

confidence: 67%

Uncoordinated expression of the main contractile and relaxation proteins in pathological skeletal muscles

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Sarco(endo)plasmic reticulum calcium pump isoforms in paralyzed rat slow muscle

Cited by 9 publications

References 28 publications

The Effect of Passive Movement on Denervated Soleus Highlights a Differential Nerve Control on SERCA and MyHC Isoforms

The Effect of Passive Movement on Denervated Soleus Highlights a Differential Nerve Control on SERCA and MyHC Isoforms

Effect of 23-day muscle disuse on sarcoplasmic reticulum Ca²⁺ properties and contractility in human type I and type II skeletal muscle fibers

Uncoordinated expression of the main contractile and relaxation proteins in pathological skeletal muscles

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Sarco(endo)plasmic reticulum calcium pump isoforms in paralyzed rat slow muscle

Cited by 9 publications

References 28 publications

The Effect of Passive Movement on Denervated Soleus Highlights a Differential Nerve Control on SERCA and MyHC Isoforms

The Effect of Passive Movement on Denervated Soleus Highlights a Differential Nerve Control on SERCA and MyHC Isoforms

Effect of 23-day muscle disuse on sarcoplasmic reticulum Ca2+ properties and contractility in human type I and type II skeletal muscle fibers

Uncoordinated expression of the main contractile and relaxation proteins in pathological skeletal muscles

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Effect of 23-day muscle disuse on sarcoplasmic reticulum Ca²⁺ properties and contractility in human type I and type II skeletal muscle fibers