Sarcodia suae modulates the immunity and disease resistance of white shrimp Litopenaeus vannamei against Vibrio alginolyticus via the purine metabolism and phenylalanine metabolism

Kuo, Chiu-Hui; Ballantyne, Rolissa; Huang, Po-Lin; Ding, Shanwu; Hong, Ming‐Chang; Lin, Tzu-Yung; Wu, Fangming; Xu, Ziyan; Chiu, Kuo-Hsun; Chen, Bonien; Liu, Chun-Hung

doi:10.1016/j.fsi.2022.07.011

Cited by 18 publications

(7 citation statements)

References 53 publications

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“…In this study, DEGs identified in the spleen between CRS and FRS significantly enriched the phenylalanine metabolic pathway. Moreover, transcriptome studies in human [ 31 ], chicken [ 32 ], fish [ 33 ], and white shrimp Litopenaeus vannamei [ 34 ] have also shown that the important role of phenylalanine metabolic pathway in the control of immune ability. Moreover, our results showed that all the DEGs (PAH, TAT, and AOC3) enriched in the phenylalanine metabolism pathway were significantly upregulated, indicating that the phenylalanine metabolism pathway was promoted in CRS geese.…”

Section: Discussionmentioning

confidence: 99%

Effects and Mechanisms of Cage versus Floor Rearing System on Goose Growth Performance and Immune Status

Tang,

Hu,

Zhang

et al. 2023

Animals

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Currently, FRS and CRS are the two predominant dryland rearing systems in the goose industry. However, the effects of these two systems on goose growth performance and health, as well as the underlying mechanisms, have not been fully clarified. Thus, this study aimed to compare growth performance and immune status, as well as investigate the genome-wide transcriptomic profiles of spleen in geese, between CRS and FRS at 270 d of age. Phenotypically, the body weight and body size traits were higher in geese under FRS, while the weight and organ index of spleen were higher in geese under CRS (p < 0.05). Noticeably, the bursa of Fabricius of geese under FRS was degenerated, while that under CRS was retained. At the serum level, the immune globulin-G (IgG) and interleukin-6 (IL-6) levels were higher in geese under CRS (p < 0.05). At the transcriptomic level, we identified 251 differentially expressed genes (DEGs) in the spleen between CRS and FRS, which were mainly enriched in scavenger receptor activity, inflammatory response, immune response, neuroactive ligand–receptor interaction, phenylalanine metabolism, ECM receptor interaction, calcium signaling pathway, phenylalanine, tyrosine, and tryptophan biosynthesis, regulation of actin cytoskeleton, and MAPK signaling pathways. Furthermore, through protein–protein interaction (PPI) network analysis, ten candidate genes were identified, namely, VEGFA, FGF2, NGF, GPC1, NKX2-5, FGFR1, FGF1, MEIS1, CD36, and PAH. Further analysis demonstrated that geese in CRS could improve their immune ability through the “phenylalanine metabolism” pathway. Our results revealed that the FRS improved growth performance, whereas the CRS improved goose immune function by increasing levels of IL-6 and IgG in serum. Moreover, the phenylalanine metabolism pathway could exert positive effects on immune function of geese under CRS. These results can provide reliable references for understanding how floor and cage rearing systems affect goose growth performance and immune capacity.

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Section: Discussionmentioning

confidence: 99%

Effects and Mechanisms of Cage versus Floor Rearing System on Goose Growth Performance and Immune Status

Tang,

Hu,

Zhang

et al. 2023

Animals

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“…L-leucine and L-lysine have physiological functions of regulating fish growth, metabolism and immune response [ 45 , 46 ]. L-tyrosine is an essential substrate of melanin synthesis, and melanin plays an important role in wound healing and antimicrobial host defense responses to promote an immune response in aquatic animals [ 47 , 48 ]. Our findings clearly revealed the disorder of amino acid metabolism in the crucian carp kidney could be induced at a low concentration of NaHCO 3 (20 mmol/L), including phenylalanine, tyrosine and tryptophan biosynthesis, tyrosine metabolism and valine, leucine and isoleucine biosynthesis ( Table S2 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of Saline-Alkaline Stress on Metabolome, Biochemical Parameters, and Histopathology in the Kidney of Crucian Carp (Carassius auratus)

et al. 2023

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The salinization of the water environment caused by human activities and global warming has increased which has brought great survival challenges to aquatic animals. Crucian carp (Carassius auratus) is an essential freshwater economic fish with superior adaptability to saline-alkali water. However, the physiological regulation mechanism of crucian carp adapting to saline-alkali stress remains still unclear. In this study, crucian carp were exposed to freshwater or 20, 40, and 60 mmol/L NaHCO3 water environments for 30 days, the effects of saline-alkali stress on the kidney were evaluated by histopathology, biochemical assays and metabolomics analysis from renal function, antioxidant capacity and metabolites level. Our results showed different degrees of kidney damage at different exposure concentrations, which were characterized by glomerular atrophy and swelling, renal tubular degranulation, obstruction and degeneration, renal interstitial edema, renal cell proliferation and necrosis. Saline-alkali stress could change the levels of several physiological parameters with renal function and antioxidant capacity, including creatinine (CREA), urea nitrogen (BUN), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA). In addition, metabolomics analysis showed that differential metabolites (DMs) were involved in various metabolic pathways, including phenylalanine, tyrosine, and tryptophan biosynthesis, aminoacyl-tRNA biosynthesis, purine metabolism, glycerophospholipid metabolism, sphingolipid metabolism, glycolysis/gluconeogenesis and the TCA cycle. In general, our study revealed that saline-alkaline stress could cause significant changes in renal function and metabolic profiles, and induce severe damage in the crucian carp kidney through destroying the anti-oxidant system and energy homeostasis, inhibiting protein and amino acid catabolism, as well as disordering purine metabolism and lipid metabolism. This study could contribute to a deeper understanding the adverse effects of saline-alkali stress on crucian carp kidney and the regulatory mechanism in the crucian carp of saline-alkali adaptation at the metabolic level.

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“…By contrast, the function of purine metabolism was uniquely coded in health cohorts (Figure S8). An induced metabolism of purine increases shrimp immune response, thus strengthening shrimp resistance to Vibro alginolyticus (Kuo et al, 2022). Following this logic, shrimp disease resistance benefits from increased purine metabolism.…”

Section: Discussionmentioning

confidence: 99%

Consistent features of the gut microbiota in response to diverse shrimp Litopenaeus vannamei diseases: A meta‐analysis

Mao,

Lu,

Chen

et al. 2023

Fish and Fisheries

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Dysbiosis in the gut microbiota has been intimately implicated in shrimp (Litopenaeus vannamei, Penaeidae) diseases. However, considering the variety of shrimp diseases and the variability in experimental conditions, studies addressing common features of the gut microbiota−shrimp disease relationship are limited. Through an unbiased subject‐level meta‐analysis framework, 463 shrimp gut bacterial communities from 27 studies were re‐analysed, including six lifestages and eight diseases of shrimp, with the causal agents of viral, bacterial, eukaryotic, and unknown pathogens. Shrimp lifestages and diseases were the predominant factors governing the gut microbiota. After ruling out the top lifestage‐ and disease‐specific discriminatory amplicon sequence variants (ASVs) from the gut microbiota, the top 27 disease common‐discriminatory ASVs were identified, contributing to an overall accuracy of 95.9% in diagnosing shrimp health status. By using these optimisation procedures, the accuracy of our diagnosis model was unbiased by shrimp lifestage, specific disease, sampling size, hypervariable region and sequencing platform. The shrimp eight diseases consistently and significantly increased the relative importance of stochasticity, the relative abundance of pathogenic potentials and diversified core ASVs, whereas decreased the diversity and stability of gut microbiota. Collectively, these findings illustrate the effectiveness of meta‐analysis in determining the robust and common features of the shrimp gut microbiota in response to diverse diseases. In particular, disease common‐discriminatory ASVs could accurately diagnose shrimp health status, although the data are divergent in biotic and technical variances.

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Sarcodia suae modulates the immunity and disease resistance of white shrimp Litopenaeus vannamei against Vibrio alginolyticus via the purine metabolism and phenylalanine metabolism

Cited by 18 publications

References 53 publications

Effects and Mechanisms of Cage versus Floor Rearing System on Goose Growth Performance and Immune Status

Effects and Mechanisms of Cage versus Floor Rearing System on Goose Growth Performance and Immune Status

Effects of Saline-Alkaline Stress on Metabolome, Biochemical Parameters, and Histopathology in the Kidney of Crucian Carp (Carassius auratus)

Consistent features of the gut microbiota in response to diverse shrimp Litopenaeus vannamei diseases: A meta‐analysis

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